Macrophage-Silicone Interactions in Women with Breast Prostheses

Hardt, Nancy S.; Emery, Jacqueline A.; LaTorre, G.P.; Batich, Chris; Winter, William E.

doi:10.1007/978-3-642-85226-8_24

“…The use of collagen is important in this model because it reproduces the in vivo environment of the silicone breast prosthesis where abundant amounts of collagen are found in the breast implant capsule 16–18. Furthermore, it has been shown that collagen promotes MDM differentiation and phagocytic capacity in vitro 24–26.…”

Section: Discussionmentioning

confidence: 99%

“…Unfortunately, clinical studies to date have been fraught with problems of bias and disease identification and have failed to find a positive connection between breast prostheses and systemic diseases of any kind 10–15. In the laboratory, histomorphologic studies of breast implants and surrounding tissues have demonstrated cellular aggregates containing silicone droplets and an inflammatory capsule containing varying amount of collagen, fibroblasts, macrophages, giant cells, and lymphocytes 16–18. In addition, many silicone products have been shown to cause activation of at least one element of the inflammatory response.…”

Section: Introductionmentioning

confidence: 99%

In vitro interaction between silicone gel and human monocyte‐macrophages

Tavazzani

¹

,

Xing

²

,

Waddell

³

et al. 2004

J Biomedical Materials Res

View full text Add to dashboard Cite

Controversy remains regarding the ability of silicone materials to induce a specific immune reaction versus a nonspecific inflammatory response. Histopathological analysis of the tissue around failed breast implants reveals chronic inflammation with silicone gel droplets either surrounded by giant cells or engulfed by macrophages, areas of fibrosis, and necrosis. Macrophages are the key cells engulfing or forming foreign body giant cells. To address the mechanisms of silicone-induced inflammation a model of human monocyte-derived macrophages (MDMs) was developed. After sonication of silicone gel, the silicone droplets were embedded in Type I collagen and used to coat glass coverslips; human MDMs were subsequently seeded on the coverslips and maintained in culture for up to 7 days. The advantage of the model was that human macrophages could be studied histologically, and cytochemically as they interacted with well-characterized silicone materials. Initial analysis of the human macrophages shows phagocytosis of the silicone gel within hours of exposure to the material. Analysis for pro-inflammatory cytokines reveals significant transient secretion of IL-1 (p < 0.01) over controls by human macrophages upon exposure to silicone gel at 24 h.

show abstract

“…These pathologies include acute inflammation, granuloma formation, immune adjuvancy, and particle dissemination. 5,7,9,10,13,[17][18][19][20]29 Because fibrinogen has been shown to act synergistically with the surface of other hydrophobic microparticulates to elicit these pathologies, 19,20 it seems reasonable to assume the same synergy operates in the case of yet another hydrophobic material, that is, of PDMS.…”

Section: Discussionmentioning

confidence: 99%

Fibrinogen adsorbs from aqueous media to microscopic droplets of poly(dimethylsiloxane) and remains coagulable

Whitlock

¹

,

Clarson

²

,

Retzinger

³

1999

J. Biomed. Mater. Res.

View full text Add to dashboard Cite

Fibrinogen binds from aqueous media containing it to droplets of linear trimethylsilyl-terminated poly(dimethylsiloxane) (PDMS) dispersed in those same media. Once bound, fibrinogen elutes from emulsified droplets of PDMS only very slowly, even when incubated in buffer that contains a physiologic concentration of the protein. The bound fibrinogen is coagulable, as indicated by the thrombin-dependent agglutination of droplets. Thus fibrinogen bound to droplets of PDMS renders an adhesive potential to the surface of the droplets, a potential that may have relevance to the biologic processing of the polymer in vivo.

show abstract

“…These pathologies include acute inflammation, granuloma formation, immune adjuvancy, and particle dissemination. 5,7,9,10,13,[17][18][19][20]29 Because fibrinogen has been shown to act synergistically with the surface of other hydrophobic microparticulates to elicit these pathologies, 19,20 it seems reasonable to assume the same synergy operates in the case of yet another hydrophobic material, that is, of PDMS.…”

Section: Discussionmentioning

confidence: 99%

Fibrinogen adsorbs from aqueous media to microscopic droplets of poly(dimethylsiloxane) and remains coagulable

Whitlock¹,

Clarson²,

Retzinger³

1999

J. Biomed. Mater. Res.

View full text Add to dashboard Cite

Fibrinogen binds from aqueous media containing it to droplets of linear trimethylsilyl-terminated poly(dimethylsiloxane) (PDMS) dispersed in those same media. Once bound, fibrinogen elutes from emulsified droplets of PDMS only very slowly, even when incubated in buffer that contains a physiologic concentration of the protein. The bound fibrinogen is coagulable, as indicated by the thrombin-dependent agglutination of droplets. Thus fibrinogen bound to droplets of PDMS renders an adhesive potential to the surface of the droplets, a potential that may have relevance to the biologic processing of the polymer in vivo.

show abstract

Macrophage-Silicone Interactions in Women with Breast Prostheses

Cited by 6 publications

References 5 publications

In vitro interaction between silicone gel and human monocyte‐macrophages

In vitro interaction between silicone gel and human monocyte‐macrophages

Fibrinogen adsorbs from aqueous media to microscopic droplets of poly(dimethylsiloxane) and remains coagulable

Fibrinogen adsorbs from aqueous media to microscopic droplets of poly(dimethylsiloxane) and remains coagulable

Contact Info

Product

Resources

About