Macrophage responses to silk

Panilaitis, Bruce; Altman, Gregory H.; Chen, Jingsong; Jin, Hyoung Joon; Karageorgiou, Vassilis; Kaplan, David L.

doi:10.1016/s0142-9612(03)00158-3

Cited by 515 publications

(402 citation statements)

References 23 publications

Supporting

Mentioning

384

Contrasting

Unclassified

Order By: Relevance

“…Silk fibroin is a biologically-derived protein polymer purified from domesticated silkworm (Bombyx mori) cocoons that has demonstrated excellent properties for biomedical applications, including biocompatibility (11)(12)(13)(14), robust mechanical strength (15), and slow, controlled degradation to nontoxic products in vivo (16). Silk can be prepared in a range of material formats, including films, hydrogels and microspheres (17,18).…”

mentioning

confidence: 99%

Stabilization of vaccines and antibiotics in silk and eliminating the cold chain

Zhang

Pritchard

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

151

147

View full text Add to dashboard Cite

Sensitive biological compounds, such as vaccines and antibiotics, traditionally require a time-dependent “cold chain” to maximize therapeutic activity. This flawed process results in billions of dollars worth of viable drug loss during shipping and storage, and severely limits distribution to developing nations with limited infrastructure. To address these major limitations, we demonstrate self-standing silk protein biomaterial matrices capable of stabilizing labile vaccines and antibiotics, even at temperatures up to 60 °C over more than 6 months. Initial insight into the mechanistic basis for these findings is provided. Importantly, these findings suggest a transformative approach to the cold chain to revolutionize the way many labile therapeutic drugs are stored and utilized throughout the world.

show abstract

mentioning

confidence: 99%

Stabilization of vaccines and antibiotics in silk and eliminating the cold chain

Zhang

Pritchard

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

151

147

View full text Add to dashboard Cite

show abstract

“…Silk fibroin, the structural, hydrophobic, and approximately 370 kDa protein isolated from the cocoon of B. mori [43][44][45][46] that has emerged as a novel substrate for engineering biomaterials, has proven to be biocompatible [22,33], offers distinguishing mechanical properties when compared to other naturally derived or degradable polymeric biomaterials [47], and exhibits low inflammatory response [21,22].…”

Section: Discussionmentioning

confidence: 99%

“…These scaffolds have low immunogenicity when properly separated from immunogenic and glycosylated proteins [19][20][21][22]. Furthermore, they can be produced in a variety of formats including electrospun nets, three-dimensional scaffolds, or tendon-like structures [23][24][25][26][27][28][29].…”

Section: Introductionmentioning

confidence: 99%

Silk based biomaterials to heal critical sized femur defects

Meinel

Betz

Fajardo

et al. 2006

Bone

218

179

View full text Add to dashboard Cite

“…Silk fibroin, another fibrous protein that closely mimics collagen type I of bone has gained popularity in tissue engineering due to interesting intrinsic properties such as resorbability [5], toughness [6], minimal immunogenicity [7,8], cytocompatibility with abundant polar, hydrophilic groups [9], strong chemical bonding with HA [10][11][12][13] and versatility in processing depending upon the target application.…”

Section: Introductionmentioning

confidence: 99%

Elucidation of differential mineralisation on native and regenerated silk matrices

Midha¹,

Tripathi²,

Geng

et al. 2016

Materials Science and Engineering: C

View full text Add to dashboard Cite

Bone mineralisation is a well-orchestrated procedure triggered by a protein-based template inducing the nucleation of hydroxyapatite (HA) nanocrystals on the matrix. In an attempt to fabricate superior nanocomposites from silk fibroin, textile braided structures made of natively spun fibres of Bombyx mori silkworm were compared against regenerated fibroin (lyophilised and films) underpinning the influence of intrinsic properties of fibroin matrices on HA nucleation. We found that native braids could bind Ca 2+ ions through electrostatic attraction, which initiated the nucleation and deposition of HA, as evidenced by discrete shift in amide peaks via ATR-FTIR. This phenomenon also suggests the involvement of amide linkages in promoting HA nucleation on fibroin. Moreover, CaCl 2 -SBF immersion of native braids resulted in preferential growth of HA along the c-axis, forming needle-like nanocrystals and possessing Ca/P ratio comparable to commercial HA. Though regenerated lyophilised matrix also witnessed prominent peak shift in amide linkages, HA growth was restricted to (211) plane only, albeit at a significantly lower intensity than braids. Regenerated films, on the other hand, provided no crystallographic evidence of HA deposition within 7 days of SBF immersion. The present work sheds light on the primary fibroin structure of B.mori which probably plays a crucial role in regulating template-induced biomineralisation on the matrix. We also found that intrinsic material properties such as surface roughness, geometry, specific surface area, tortuosity and secondary conformation exert influence in modulating the extent of mineralisation. Thus our work generates useful insights and warrants future studies to further investigate the potential of bone mimetic, silk/mineral nanocomposite matrices for orthopaedic applications.

show abstract

Macrophage responses to silk

Cited by 515 publications

References 23 publications

Stabilization of vaccines and antibiotics in silk and eliminating the cold chain

Stabilization of vaccines and antibiotics in silk and eliminating the cold chain

Silk based biomaterials to heal critical sized femur defects

Elucidation of differential mineralisation on native and regenerated silk matrices

Contact Info

Product

Resources

About