Macrophage responses to 316L stainless steel and cobalt chromium alloys with different surface topographies

Anderson, Jordan A.; Lamichhane, Sujan; Mani, Gopinath

doi:10.1002/jbm.a.35808

Cited by 23 publications

(24 citation statements)

References 49 publications

(151 reference statements)

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“…It happens as a result of the biological response of macrophages over the biomaterial. Macrophages are body cells which induce bone growth, and their response is scientifically proved as better in biomaterials with pores and consequent rough surface, such as those produced by the powder metallurgy route (Silva et al, 2014;Silva, 2001;Anderson et al, 2016).…”

Section: Resultsmentioning

confidence: 99%

Study of the microstructure and mechanical properties of beta tricalcium phosphate-based composites with alumina addition produced by powder metallurgy

et al. 2017

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The use of alumina as a reinforcement in metallic and ceramic matrix is wellknown. The purpose of this study was to investigate the effect of alumina addition according to its amount on the microstructural and mechanical behavior of tricalcium phosphate ceramic (TCP), more specifically the β-TCP. Although β-TCP has excellent bioactive and biocompatible properties, it presents low fracture resistance and load bearing capacity, which limits its use in the monolithic form. The route used for the β-TCP/alumina composite production was powder metallurgy. The powders were milled in a high energy ball mill with the following parameters: 5, 10, 15 and 20 hours, mass/sphere relation of 1:20 and speed of 150 rpm. After the milling process, the powders of both compositions were uniaxially pressed and sintered. Two of the compositions were processed containing 90% and 50% of β-TCP, respectively. The results indicated that composition 1 with a smaller percentage of alumina in its microstructure presented 62.4 % smaller particle size after the high energy ball milling process, which provided higher densification after pressing and sintering. These results implied in increased mechanical resistance, with 4.6 GPa of elastic modulus and 140 MPa of compressive strength, against 3.6 GPa of elastic modulus and 117 MPa of compressive strength obtained in composition 2.

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Section: Resultsmentioning

confidence: 99%

Study of the microstructure and mechanical properties of beta tricalcium phosphate-based composites with alumina addition produced by powder metallurgy

et al. 2017

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“…The toxicity of cobalt has been partly attributed to generated cobalt ions, which are likely to stimulate the generation of reactive oxygen species (ROS) in different cells . Moreover, Co‐containing nanoscale particles can be incorporated by immune cells, for example, macrophages, thereby inducing the release of pro‐inflammatory mediators (e.g., interleukins and TNFα) . Furthermore, it is suggested that the formation of free radicals plays a role in the process of periprosthetic fibrosis and loosening of hip arthroplasty .…”

Section: Introductionmentioning

confidence: 99%

Ionic cobalt but not metal particles induces ROS generation in immune cells in vitro

et al. 2018

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Total joint replacement is one of the most successful procedures in orthopedic surgery today. However, metal implant materials undergo wear and corrosion processes. Generated particles and ions can cause a variety of cellular reactions. Cobaltcontaining alloys are used frequently in implant materials. Some studies suggest that cobalt exhibits potential cytotoxic effects, for example, via generation of reactive oxygen species (ROS). To further elucidate the effects of cobalt on human cells, we determined cell viability and cytosolic and mitochondrial superoxide formation after incubation of either ions or particles with different cells. MM-6 and Jurkat cell lines were treated for 24, 48 and 72 h with either CoCrMo particles or cobalt ions (supplied as CoCl 2 ). A total of 24 h exposure of both forms of cobalt did not induce cell death using terminal deoxynucleotidyl transferase (TUNEL) and trypan blue assay. Interestingly, the formation of superoxide (O 2 . − ) is evoked mainly by ionic CoCl 2 but not cobalt particles. Cobalt alloy particles are likely to even suppress O 2 .− formation in mitochondria in both used cell lines. Furthermore, we did not observe any effect of cobalt particles on O 2 .− formation in peripheral blood mononuclear cells (PBMCs) from healthy donors. We also found that the O 2 − formation by CoCl 2 within mitochondria is a generalized effect for all cell types used, while the formation of superoxide in cytosolic compartment is cell-type dependent. In summary, our data suggest that cobalt ions specifically induce the formation of O 2 .−, whereas the cobalt particles were better tolerated.

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“…Cobalt–chromium–molybdenum (CoCrMo) alloy has been in use as a biomaterial for nearly 90 years due to its advanced ability to resist wear and corrosion when compared to other alloys, however, CoCrMo is susceptible to many types of corrosion typically associated with mechanically assisted crevice corrosion (MACC) where the environment may be chemically and/or mechanically aggressive . During MACC, electrode potentials at the CoCrMo surface can shift as far negative as −900 mV (vs. Ag/AgCl) from an unabraded open circuit potential (OCP) of about −250 mV (vs. Ag/AgCl) .…”

Section: Introductionmentioning

confidence: 99%

“…Surface roughness and morphology has also been documented to impact inflammatory cell activation responses. Anti‐inflammatory cytokine (IL‐10) production is favored on microrough CoCrMo surfaces whereas pro‐inflammatory cytokine (TNF‐α, IL‐6, and IP‐10) production is favored on smooth CoCrMo surfaces …”

Section: Introductionmentioning

confidence: 99%

“…Anti-inflammatory cytokine (IL-10) production is favored on microrough CoCrMo surfaces whereas pro-inflammatory cytokine (TNF-α, IL-6, and IP-10) production is favored on smooth CoCrMo surfaces. 3 Reactive oxygen species (ROS) are another set of chemically active molecules that can contribute to oxidative stress on the cell as well as structural damage to the alloy surface. ROS-like superoxide radicals (O 2…”

Section: Introductionmentioning

confidence: 99%

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Electrochemical potential zone of viability on CoCrMo surfaces is affected by cell type: Macrophages under cathodic bias are more resistant to killing

Wiegand

Kubacki

Gilbert

2018

J Biomedical Materials Res

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Electrochemical interactions at the cell–metal interface determine cell viability and influence behavior in response to different electrode potential conditions, specifically cathodic biases. Mechanically assisted crevice corrosion, for example, induces cathodic potentials and the associated electrochemical consequences of increased reduction reactions at the implant surface may affect cell viability in a manner that is different for various cell phenotypes. Monocyte macrophage‐like U937 cells were cultured on cobalt–chromium–molybdenum (CoCrMo) metal surfaces in vitro for 24 h to assess cell behavior in response to sustained applied voltages. The electrochemical zone of viability for U937 cells polarized for 24 h in vitro was −1000 ≤ mV < +500, compared to −400 < mV < +500 for MC3T3‐E1 preosteoblast‐like cells cultured under the same conditions, likely as a result of intrinsic apoptosis. Voltages above +250 mV had a lethal effect on U937 cells that was similar to that seen previously for MC3T3‐E1 cells on biased CoCrMo surfaces. It appears that cell phenotype directly influences behavior in response to cathodic electrochemical stimuli and that the monocyte macrophage‐like cells are more resistant to cathodic potential stimuli than preosteoblasts. This may be due to a glutathione‐based increased ability to quench reactive oxygen species and inflammatory‐associated radicals hypothesized to be generated during reduction of oxygen. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 526–534, 2019.

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Macrophage responses to 316L stainless steel and cobalt chromium alloys with different surface topographies

Cited by 23 publications

References 49 publications

Study of the microstructure and mechanical properties of beta tricalcium phosphate-based composites with alumina addition produced by powder metallurgy

Study of the microstructure and mechanical properties of beta tricalcium phosphate-based composites with alumina addition produced by powder metallurgy

Ionic cobalt but not metal particles induces ROS generation in immune cells in vitro

Electrochemical potential zone of viability on CoCrMo surfaces is affected by cell type: Macrophages under cathodic bias are more resistant to killing

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