Macrophage polarization in kidney transplant patients

Devraj, Vijaya Madhuri; Kalidindi, Karthik; Swarnalatha, G; Uppin, Megha S; Taduri, Gangadhar

doi:10.1016/j.trim.2022.101717

Cited by 3 publications

(3 citation statements)

References 29 publications

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“…It was shown that the levels of M1 macrophages, T cells, CD8 T cells and gamma delta T cells were signi cantly increased in rejected kidneys compared to nonrejected kidneys [28] . Consistent with our ndings, it has been shown that an increase in M1-type macrophages predominates in the rejection of transplanted kidneys [32] . After kidney transplantation, CD8 T cells play an important role in the process of allograft rejection by invading allograft tissues and activating other types of immune cells, and the phenotypic and molecular characteristics of CD8 T cells correlate signi cantly with the development of the T-cellmediated immune rejection response (TCMR) [33] .…”

Section: Discussionsupporting

confidence: 93%

Landscape of the Immune Infiltration and Identification of Molecular Diagnostic Markers Associated With Immune Cells in Patients With Kidney Transplantation

Xu,

Sun,

et al. 2024

Preprint

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Rejection seriously affects the success of kidney transplantation. However, the molecular mechanism of the occurrence of rejection remains unclear. Firstly, GSE21374 and GSE36059 dataset were downloaded from the Gene Expression Omnibus (GEO) database. Next, Cell-type Identification by Estimating Relative Subsets of RNA Transcripts (CIBERSORT) algorithm was selected to infer the proportions of 22 immune cells. Moreover, infiltrating immune cells-related genes were identified by weighted gene co-expression network analysis (WGCNA), and enrichment analysis was conducted to observe their biological function. Furthermore, Extreme Gradient Boosting (XGBoost) and Least Absolute Shrinkage and Selection Operator (LASSO) logistic regression algorithm was selected to screen hub genes. Ultimately, quantitative real-time-PCR were conducted to verify the numbers of immune cells and the expressions of hub genes. Down-regulated B cells memory, Plasma cells, and Mast cell and up-regulated T cells follicular helper, T CD8 cells, Macrophages M1, T Cells CD4 memory activated, and T cells gamma delta were up-regulated were observed in rejections. Subsequently, ARS, CD8A, CRTAM, GBP2 and VAMP5 were screened as hub genes by XGBoost and LASSO algorithm, and might be used to the diagnostic biomarkers. Finally, differential analysis and quantitative real-time-PCR suggested that ARS, CD8A, CRTAM, GBP2 and VAMP5 were up-regulated in rejection samples compared to non-rejection samples. The present study identified 5 key infiltrating immune cells-related genes (ARS, CD8A, CRTAM, GBP2 and VAMP5) in rejection of kidney transplantation, which may contribute to explain the molecular mechanism of rejection in kidney transplantation development.

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Section: Discussionsupporting

confidence: 93%

Landscape of the Immune Infiltration and Identification of Molecular Diagnostic Markers Associated With Immune Cells in Patients With Kidney Transplantation

Xu,

Sun,

et al. 2024

Preprint

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“…Previous studies indicated that resveratrol can inhibit the secretion of pro-inflammatory cytokines, such as IL-1, IL-6, and TNF-α, and increase the opposite effect of IL-10 produced by mouse macrophages, thereby regulating the activity of T cells and B cells ( 40 , 41 ). M1 macrophages can secrete iNOS, IL-6, IL-1β, and TNF-α, which are pro-inflammatory cytokines associated with allograft rejection ( 39 , 42 ). MCP-1, another important pro-inflammatory cytokine, can be secreted by macrophages during inflammation, significantly impacting the migration and infiltration of macrophages ( 43–45 ).…”

Section: Discussionmentioning

confidence: 99%

Resveratrol regulates macrophage recruitment and M1 macrophage polarization and prevents corneal allograft rejection in rats

Xu,

Guo,

Hou

et al. 2023

Front. Med.

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IntroductionResveratrol is an immune modulator that can reduce M1 macrophage polarization in vitro. Reducing macrophage recruitment and M1 polarization can prevent corneal allograft rejection (CGR). In this study, rat corneal allograft rejection models were established to explore the effects of resveratrol on CGR and macrophages and the underlying mechanisms after corneal transplantation.MethodsCorneal allograft models were established, and 100 mg/kg resveratrol was injected intraperitoneally. The corneal allografts were assessed clinically using the Holland rejection scoring system, anterior segment photography, and anterior segment optical coherence tomography. Corneal macrophages, pro-inflammatory cytokines, and corneal lymphatic vessels were detected using hematoxylin and eosin staining, immunofluorescence staining, and real-time quantitative polymerase chain reaction (qRT-PCR). Dendritic cells (DCs) in cervical lymph nodes were explored using flow cytometry. RNA sequencing experiments were conducted to identify the mechanisms through which resveratrol affected CGR. The results were verified using Simple Western analysis. Pro-inflammatory cytokines by macrophages in vitro were measured using qRT-PCR and enzyme-linked immunosorbent assays.ResultsResveratrol significantly prolonged the survival of corneal grafts and reduced graft edema and central corneal thickness. Corneal macrophage recruitment and M1 macrophage polarization decreased significantly after corneal transplantation in the resveratrol group. Resveratrol also reduced pro-inflammatory cytokines in corneal grafts and suppressed the early generation of cornea lymphatic vessels and the recruitment of cornea inflammatory cells 14 days after surgery. Resveratrol decreased the proportion of DCs in ipsilateral cervical lymph nodes. The effect of resveratrol on CGR was related to the phosphatidylinositol 3-kinase/protein kinase-B (PI3K/Akt) pathway. Resveratrol reduced the secretion of pro-inflammatory cytokines by M1 macrophages in vitro.ConclusionOur findings suggest that resveratrol can reduce corneal macrophage recruitment and M1 macrophage polarization after corneal transplantation in rats and prevent CGR. The PI3K/Akt pathway may be an important mechanism that warrants further research.

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“…The mechanisms of macrophage participation in the development of CAN are complex, but the proinflammatory effect mediated by cytokines (TNF-α, IL-1) and cell damage by products of oxidative stress reactions are mentioned as probable mechanisms [31,32]. Activated macrophages synthesize a large number of proinflammatory cytokines including IL-1, IL-18, and TNF-α [32,33]. Interleukin 1 activates endothelial cells and induces the production of other cytokines, chemokines, and leukocyte adhesion molecules [34].…”

Section: Discussionmentioning

confidence: 99%

Cytokine Profile in Renal Transplant Patients in a Long-Term Period After Surgery

et al. 2023

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In the long-term period after kidney transplantation, a certain level of tissue inflammation and therefore the production of proinflammatory cytokines, including TNF-α, IL-1β, IL-18 and IL-2 can be found. The aim of our study was to determine the concentrations of TNF-α, IL-1β, IL-18, IL-2 and its soluble receptor (IL-2R) in renal transplant patients, regarding the length of the postoperative period. The study involved 65 patients, transplanted at least 12 months prior to our investigation, divided into three groups, regarding the time passed since the transplantation (12-24, 24-48, and >48 months consecutively). Concentrations of the cytokines in the plasma of the subjects were measured using ELISA method. Group I showed significantly higher concentrations of IL-1b compared to the III (p<0.05), IL-18 compared to the II and III (p<0.05) and TNF-a compared to the II (p<0.05). Cytokine concentrations correlated with the time passed since the transplantation (p<0.05), except for TNF-a. Interleukin-2 correlated negatively with IL-18 and immunosuppressant dosage (p<0.05). Interleukin-1b, IL-18 and TNF-α measurements should be considered for monitoring and detecting potentially subclinical allograft damage in the second year after surgery. However, the dynamics of the change of cytokine concentration may also have been altered by the components of the immunosuppressive protocols used, such as tacrolimus, which is a link that is yet to be examined.

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Macrophage polarization in kidney transplant patients

Cited by 3 publications

References 29 publications

Landscape of the Immune Infiltration and Identification of Molecular Diagnostic Markers Associated With Immune Cells in Patients With Kidney Transplantation

Landscape of the Immune Infiltration and Identification of Molecular Diagnostic Markers Associated With Immune Cells in Patients With Kidney Transplantation

Resveratrol regulates macrophage recruitment and M1 macrophage polarization and prevents corneal allograft rejection in rats

Cytokine Profile in Renal Transplant Patients in a Long-Term Period After Surgery

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