Macrophage migration inhibitory factor promoter polymorphisms (−794 CATT5–8): Relationship with soluble MIF levels in coronary atherosclerotic disease subjects

Abstract

BackgroundWe analyzed the relationship of −794 CATT5–8 MIF polymorphisms with soluble MIF in Coronary Atherosclerotic Disease (CAD) patients.MethodsA total of 256 patients selected, on which 186 normal-coronary and 70 Coronary artery disease subjects, were recruited in the study (Retrospectively registered). Genotyping of −794 CATT5–8 polymorphisms were performed by PCR and DNA sequencing. Serum MIF levels were measured using an ELISA kit. Patients were classified by coronary angiogram, and CAD based on Gensini’s integral degree (angiographic scoring system).ResultsThe allele frequency and genotype frequency of −794 CATT5–8 did not show any differences in normal-coronary subjects and CAD subjects. In CAD patients, serum MIF levels was lower in CATT (5) subjects than in CATT (7) subjects, while the genotype of −794 CATT5–8 did not show differences in serum MIF levels. In addition, we found a decrease in serum MIF levels in carriers of the (5/5) genotypes the −794 CATT5–8 MIF polymorphisms, although it was not significant. There was no relationship of CAD class and the allele frequency of −794 CATT5–8.ConclusionsThis study found no association between CAD class and −794 CATT5–8 MIF polymorphisms with soluble MIF levels in CAD Subjects.Trial registrationNCT01750502 (November 2012, Retrospectively registered).

MIF gene rs755622 polymorphism positively associated with acute coronary syndrome in Chinese Han population: case–control study

“…A study conducted in Czech and Russian population failed to detect a significant difference in the distribution of MIF -173G/C genotypes, alleles or carriage rates between patients with myocardial infarction and control groups 38 . The lack of association between CAD class and MIF -794 (CATT) 5-8 polymorphisms with soluble MIF levels in a small number of CAD subjects (n = 70) was reported 39 . Although the reason for these discrepant results is not defined, it may be due to the difference in the stage of CAD and phenotypic variance, therefore a large scare of clinical study is warranted to confirm the universal implication of MIF gene polymorphism in predicting the risk of CAD.…”

Pirfenidone suppresses polarization to M2 phenotype macrophages and the fibrogenic activity of rat lung fibroblasts

“…Macrophages can influence a variety of pathologies and are able acquire several phenotypes. ( 27 – 29 ) Among the various macrophage phenotypes, the M2 phenotype has been shown to be associated with the secretion of pro-fibrotic factors and the promotion the fibroblast proliferation. ( 19 ) M2 macrophage numbers have been reported to be elevated in lung tissue in a mouse model of pulmonary fibrosis.…”

The Macrophage Migration Inhibitory Factor (MIF) Promoter Polymorphisms (rs3063368, rs755622) Predict Acute Kidney Injury and Death after Cardiac Surgery

“…The MIF promoter microsatellite was suggested to influence MIF mRNA expression, as assessed by luciferase reporter assays in normal and patient cells [ 11 , 14 ]. The longer CATT 7 repeat further has been associated with higher serum MIF levels in various patient cohorts, including those with coronary artery disease [ 10 , 29 , 30 ]. In this study, we found that patients carrying the CATT 7 allele had higher serum MIF preoperatively and one hour after surgery, but serum MIF did not differ at the other intra- and postoperative timepoints.…”