Macrophage migration inhibitory factor (MIF) and pregnancy may impact the balance of intestinal cytokines and the development of intestinal pathology caused by Toxoplasma gondii infection

Marcon, Camila Ferreira; Ferreira, Paula Tatiana Mutão; Franco, Priscila Silva; Ribeiro, Mayara; Silva, Rafaela José; Sousa, Roberto Augusto Pereira; Oliveira, Carlo José Freire; Rodrigues, Virmondés; Gomes, Marcos de Lucca Moreira; Chica, Javier Emílio Lazo; Mineo, Tiago Wilson Patriarca; Barbosa, Bellisa Freitas; Ferro, Eloísa Amália Vieira; Gomes, A. da S.

doi:10.1016/j.cyto.2020.155283

Cited by 7 publications

(2 citation statements)

References 52 publications

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“…Moreover, there is an inability to undergo normal expansion of eosinophils and macrophages [47]. Marcon et al discovered that the absence of MIF and pregnancy increase the severity of Toxoplasma gondii infection, leading to a decrease in intestinal villi and an increase in mucosal cell numbers, while also inducing a strong Th1 inflammatory response, which is further exacerbated by the lack of MIF [48]. Therefore, MIF may play a crucial role in the immune response of goats infected with H. contortus.…”

Section: Discussionmentioning

confidence: 99%

Single-Cell Transcriptomic Profiling Unveils Dynamic Immune Cell Responses during Haemonchus contortus Infection

Wang,

Jin,

Kong

et al. 2024

Cells

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Background: Haemonchus contortus is a parasite widely distributed in tropical, subtropical, and warm temperate regions, causing significant economic losses in the livestock industry worldwide. However, little is known about the genetics of H. contortus resistance in livestock. In this study, we monitor the dynamic immune cell responses in diverse peripheral blood mononuclear cells (PBMCs) during H. contortus infection in goats through single-cell RNA sequencing (scRNA-Seq) analysis. Methods and Results: A total of four Boer goats, two goats with oral infection with the L3 larvae of H. contortus and two healthy goats as controls, were used in the animal test. The infection model in goats was established and validated by the fecal egg count (FEC) test and qPCR analysis of the gene expression of IL-5 and IL-6. Using scRNA-Seq, we identified seven cell types, including T cells, monocytes, natural killer cells, B cells, and dendritic cells with distinct gene expression signatures. After identifying cell subpopulations of differentially expressed genes (DEGs) in the case and control groups, we observed the upregulation of multiple inflammation-associated genes, including NFKBIA and NFKBID. Kyoto Encyclopedia of the Genome (KEGG) enrichment analysis revealed significant enrichment of NOD-like receptor pathways and Th1/Th2 cell differentiation signaling pathways in CD4 T cells DEGs. Furthermore, the analysis of ligand–receptor interaction networks showed a more active state of cellular communication in the PBMCs from the case group, and the inflammatory response associated MIF–(CD74 + CXCR4) ligand receptor complex was significantly more activated in the case group, suggesting a potential inflammatory response. Conclusions: Our study preliminarily revealed transcriptomic profiling characterizing the cell type specific mechanisms in host PBMCs at the single-cell level during H. contortus infection.

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Section: Discussionmentioning

confidence: 99%

Single-Cell Transcriptomic Profiling Unveils Dynamic Immune Cell Responses during Haemonchus contortus Infection

Wang,

Jin,

Kong

et al. 2024

Cells

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“…MIF, a multifunctional cytokine, is involved in the development of gut‐related inflammation, 72 and it is an important regulator of macrophage responses to gram‐negative bacteria 73 . Moreover, the majority of intestinal bacteria are gram‐negative bacteria 74 .…”

Section: Discussionmentioning

confidence: 99%

The impact of MIF deficiency on alterations of fecal microbiota in C57BL/6 mice induced by Trichinella spiralis infection

Xie,

Xu,

Yan

et al. 2023

The FASEB Journal

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Trichinellosis caused by Trichinella spiralis (T. spiralis) is a major food‐borne parasitic zoonosis worldwide. Prevention of trichinellosis is an effective strategy to improve patient quality of life. Macrophage migration inhibitory factor (MIF) is closely related to the occurrence and development of several parasitic diseases. Studying the impact of MIF deficiency (Mif−/−) on the alterations in host fecal microbiota due to T. spiralis infection may contribute to proposing a novel dual therapeutic approach for trichinellosis. To reveal the diversity and differences in fecal microbial composition, feces were collected from T. spiralis‐uninfected and T. spiralis‐infected wild‐type (WT) and MIF knockout (KO) C57BL/6 mice at 0, 7, 14, and 35 days post‐infection (dpi), and the samples were sent for 16S rRNA amplicon sequencing on the Illumina NovaSeq platform. Flow cytometry was used to determine the expression levels of IFN‐γ and IL‐4 in the CD4+/CD8+ T‐cell sets of mouse spleens. The results showed that operational taxonomic unit (OTU) clustering, relative abundance of microbial composition, alpha diversity, and beta diversity exhibited significant changes among the eight groups. The LEfSe analysis selected several potential biomarkers at the genus or species level, including Akkermansia muciniphila, Lactobacillus murinus, Coprococcus catus, Firmicutes bacterium M10_2, Parabacteroides sp. CT06, and Bacteroides between the KTs and WTs groups. The predicted bacterial functions of the fecal microbiota were mainly involved in metabolism, such as the metabolism of carbohydrates, amino acids, energy, cofactors, vitamins, nucleotides, glycans, and lipids. Flow cytometry revealed an increased CD3+CD8−/CD3+CD8+ T‐cell ratio and increased IFN‐γ and IL‐4 levels in CD3+CD8− T‐cell sets from WT and MIF KO mice at 7 dpi. The results indicated that both MIF KO and infection time have a significant influence on the CD3+CD8−IFN‐γ+ and CD3+CD8−IL‐4+ response in mice after T. spiralis. In conclusion, this research showed alterations of the fecal microbiota and immune response in both WT and MIF KO mice before and after T. spiralis infection. These results revealed a potential role of MIF in regulating the pathogenesis of trichinellosis related to the intestinal microbiota. Importantly, the selected potential biomarkers combined with MIF will also offer a novel therapeutic approach to treat trichinellosis in the future.

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Influence of interleukin 17 A and 17 F polymorphisms in keratoconus

et al. 2021

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Macrophage migration inhibitory factor (MIF) and pregnancy may impact the balance of intestinal cytokines and the development of intestinal pathology caused by Toxoplasma gondii infection

Cited by 7 publications

References 52 publications

Single-Cell Transcriptomic Profiling Unveils Dynamic Immune Cell Responses during Haemonchus contortus Infection

Single-Cell Transcriptomic Profiling Unveils Dynamic Immune Cell Responses during Haemonchus contortus Infection

The impact of MIF deficiency on alterations of fecal microbiota in C57BL/6 mice induced by Trichinella spiralis infection

Influence of interleukin 17 A and 17 F polymorphisms in keratoconus

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