Macrophage Migration Inhibitory Factor Inhibits the Migration of Cartilage End Plate-Derived Stem Cells by Reacting CD74

Xiong, Chengjie; Huang, Bo; Zhou, Yue; Cun, Yanping; Liu, L.; Wang, J.; Li, C.; Pan, Yong; Wang, H.

doi:10.1055/s-0034-1376633

Cited by 4 publications

(6 citation statements)

References 39 publications

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“…S2B–S2D). The cells expressing MT‐MMP1, Stro‐1, and CD105 were regarded as human CESCs which possess the migratory capacity . This suggests that all the cell types we have found in rabbit CEPs also exist in mature adult CEPs.…”

Section: Discussionmentioning

confidence: 77%

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Collagen-DerivedN-Acetylated Proline-Glycine-Proline in Intervertebral Discs Modulates CXCR1/2 Expression and Activation in Cartilage Endplate Stem Cells to Induce Migration and Differentiation Toward a Pro-Inflammatory Phenotype

et al. 2015

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The factors that regulate the migration and differentiation of cartilage endplate stem cells (CESCs) remain unknown. N-Acetylated proline-glycine-proline (N-Ac-PGP) is a chemokine that is involved in inflammatory diseases. The purpose of this study was to detect N-Ac-PGP in degenerative intervertebral discs (IVDs) and to determine its roles in the migration and differentiation of CESCs. Enzyme-linked immunosorbent assay (ELISA) and liquid chromatography-mass spectrometry results indicated that the levels of the proteases that generate N-Ac-PGP as well as NAc-PGP levels themselves increase with the progression of IVD degeneration. Immunohistochemistry and an N-Ac-PGP generation assay demonstrated that nucleus pulposus (NP) cells generate N-Ac-PGP from collagen. The effects of N-Ac-PGP on the migration and differentiation of CESCs were determined using migration assays, RT-PCR, immunoblot analysis, and ELISA. The results showed that the expression of N-Ac-PGP receptors (CXCR1 and CXCR2) in CESCs was upregulated by N-Ac-PGP. Additionally, N-Ac-PGP induced F-actin cytoskeletal rearrangement in CESCs and increased CESC chemotaxis. Furthermore, N-Ac-PGP recruited chondrocytes and spindle-shaped cells from the cartilage endplate (CEP) into the NP in vivo. These spindle-shaped cells expressed CD105 and Stro-1 (mesenchymal stem cell markers). N-Ac-PGP induced the differentiation of CESCs toward a pro-inflammatory phenotype with increased production of inflammatory cytokines rather than toward an NP-like phenotype. Our study indicated that, in the complex microenvironment of a degenerative disc, N-Ac-PGP is generated by NP cells and induces the migration of CESCs from the CEP into the NP. N-Ac-PGP induces a pro-inflammatory phenotype in CESCs, and these cells promote the inflammatory response in degenerative discs.

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Section: Discussionmentioning

confidence: 77%

“…The conditioned media were also harvested for enzyme‐linked immunosorbent assay (ELISA). CESCs were identified by flow cytometry analysis as previously described (data not shown) . The cells were used in experiments at the second passage.…”

Section: Methodsmentioning

confidence: 99%

Collagen-DerivedN-Acetylated Proline-Glycine-Proline in Intervertebral Discs Modulates CXCR1/2 Expression and Activation in Cartilage Endplate Stem Cells to Induce Migration and Differentiation Toward a Pro-Inflammatory Phenotype

et al. 2015

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“…Intracellular chloride regulates cation transport and may be involved in cellular signaling; CLIC4 expression has been reportedly associated with Ca 2+ induced differentiation of keratinocytes [71, 72]. Macrophage migration inhibitory factor (MIF) is elevated during tissue injury and inhibits MSC migration [73, 74]. Our data showed downregulation of MIF expression [Figure 6.2].…”

Section: Resultsmentioning

confidence: 69%

SalmonellaEnhances Osteogenic Differentiation in Adipose-Derived Mesenchymal Stem Cells

Foutouhi¹,

Mohamad‐Fauzi²,

Storey³

et al. 2019

Preprint

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22 and interpretation; DB: Study design, Provision of study material; JDM: Experiment conception and design, 23Financial support, Administrative support, Provision of study material, Data analysis and interpretation, Manuscript24 writing, Final approval of manuscript.; BCW: Experiment conception and design, Financial support, 25 Administrative support, Provision of study material, Data analysis and interpretation, Manuscript writing, Final 26 approval of manuscript. 28Abstract 29

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“…Most cytokines in CM, such as CCL2, CXCL1, CXCL10, and IL-8, have been reported to promote the migration of MSCs 2,5,28,31 ; MIF, on the contrary, has been proven to have a negative effect on the migration of MSCs. 40 Therefore, the combination of chemoattractive and antichemoattractive cytokines, the source of MSCs, culture medium, and treatment time might explain the different results. Nonetheless, our results suggested that the migration ability of injected IPFP MSCs toward the defect region might be impaired in vivo by secreted factors from OA cartilage.…”

Section: Discussionmentioning

confidence: 99%

Effects of Conditioned Medium From Osteoarthritic Cartilage Fragments on Donor-Matched Infrapatellar Fat Pad–Derived Mesenchymal Stromal Cells

Song

Guo

et al. 2019

Am J Sports Med

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Background: Mesenchymal stromal cell (MSC)–based therapies have emerged as a promising strategy for osteoarthritis (OA) treatment. In particular, infrapatellar fat pad (IPFP)–derived MSCs have become a good option to treat knee OA. Purpose: To investigate the influence of the local microenvironment of the knee joint, especially OA cartilage, on the bioactivities of injected/implanted IPFP MSCs. Study Design: Controlled laboratory study. Methods: Conditioned medium (CM) derived from OA cartilage fragments was collected and characterized. Donor-matched IPFP MSCs were treated with control medium (Dulbecco’s modified Eagle medium (DMEM)/F-12 or chondrogenic medium), control medium + CM, or CM alone; and a series of behaviors including the viability, migration, chondrogenic and hypertrophic differentiation, and catabolic activity of IPFP MSCs were evaluated among groups. Results: There were 14 cytokines detected in CM. CM treatment improved the viability of IPFP MSCs. CM hindered the migration of IPFP MSCs. In chondrogenic differentiation, the presence of CM increased the expression of chondrogenic markers but also enhanced the state of hypertrophy and catabolism. Conclusion: OA cartilage–secreted factors could induce chondrogenic differentiation but also resulted in negative effects including the weakened migration, increased hypertrophy, and catabolism of IPFP MSCs in vitro. Clinical Relevance: These findings provide an insight on the fate of IPFP MSCs after intra-articular injections.

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Macrophage Migration Inhibitory Factor Inhibits the Migration of Cartilage End Plate-Derived Stem Cells by Reacting CD74

Cited by 4 publications

References 39 publications

Collagen-DerivedN-Acetylated Proline-Glycine-Proline in Intervertebral Discs Modulates CXCR1/2 Expression and Activation in Cartilage Endplate Stem Cells to Induce Migration and Differentiation Toward a Pro-Inflammatory Phenotype

Collagen-DerivedN-Acetylated Proline-Glycine-Proline in Intervertebral Discs Modulates CXCR1/2 Expression and Activation in Cartilage Endplate Stem Cells to Induce Migration and Differentiation Toward a Pro-Inflammatory Phenotype

SalmonellaEnhances Osteogenic Differentiation in Adipose-Derived Mesenchymal Stem Cells

Effects of Conditioned Medium From Osteoarthritic Cartilage Fragments on Donor-Matched Infrapatellar Fat Pad–Derived Mesenchymal Stromal Cells

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