Macrophage migration inhibitory factor as a potential biomarker of endometriosis

Mahdian, Soodeh; Aflatoonian, Reza; Yazdi, Reza Salman; Yaghmaei, Parichehreh; Ramazanali, Fariba; Afsharian, Parvaneh; Shahhoseini, Maryam

doi:10.1016/j.fertnstert.2014.09.031

Cited by 24 publications

(16 citation statements)

References 28 publications

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“…Following activation, these cells secrete proinflammatory cytokines (21,22), chemotactic and angiogenic proteins, and the macrophage migration inhibition factor (MIF) (23). Among them, TNF-a, CD74, and MIF are able to initiate signaling cascades that lead to the proliferation and survival of endometriotic implants (24). Since the cytotoxicity of the natural killer (NK) cells (25,26) and the T-cell-mediated adaptive immune response is also defective in endometriosis, it further aids the survival of ectopic implants (15,19,27).…”

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confidence: 99%

Serum galectin-9 as a noninvasive biomarker for the detection of endometriosis and pelvic pain or infertility-related gynecologic disorders

Brubel

Bokor

Pohl

et al. 2017

Fertility and Sterility

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confidence: 99%

Serum galectin-9 as a noninvasive biomarker for the detection of endometriosis and pelvic pain or infertility-related gynecologic disorders

Brubel

Bokor

Pohl

et al. 2017

Fertility and Sterility

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“…The assessment of the certain gene alteration patterns in women with fertility disorders such as patients affected by endometriosis and understanding how this disease disrupts all aspects of the reproductive process could improve the molecular insights into the reasons for their infertility [47]. Some studies showed that the intrinsic ovarian problems is accompanied with reduced endometrial receptivity, and this is the cause of low rate of pregnancy in endometriosis patients [48,49].…”

Section: Discussionmentioning

confidence: 99%

Epigenetic alterations of CYP19A1 gene in Cumulus cells and its relevance to infertility in endometriosis

Hosseini

Mehraein

Shahhoseini

et al. 2016

J Assist Reprod Genet

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Purpose The purpose of the present study was to investigate the epigenetic mechanisms responsible for the aberrant aromatase expression (CYP19A1) in Cumulus Cells (CCs) of infertile endometriosis patients. Method Cumulus cells were obtained from 24 infertile patients with and without endometriosis who underwent ovarian stimulation for intracytoplasmic sperm injection. Expression of CYP19A1 gene was quantified using Reverse Transcription Q-PCR. DNA methylation, histone modifications, and binding of Estrogen Receptor, ERβ to regulatory DNA sequences of CYP19A1 gene were evaluated by Chromatin ImmunoPrecipitation (ChIP) assay.Results CYP19A1 gene expression in CCs of endometriosis patients was significantly lower than the control group (P = 0.04). Higher incorporation of MeCP2 (as a marker of DNA methylation) on PII and PI.4 promoters, and hypoacetylation at H3K9 in PII and hypermethylation at H3K9 in PI.4 were observed in CYP19A1 gene in endometriosis patients (P < 0.05). Moreover, a decreased level of ERβ binding to PII and an increased level of its binding to PI.3 and PI.4 promoters of CYP19A1 were observed in endometriosis patients when compared to control. Conclusion Significant reduction of CYP19A1 gene expression in CCs of endometriosis patients may be the result of epigenetic alterations in its regulatory regions, either by DNA methylation or histone modifications. These epigenetic changes along with differential binding of ERβ (as a transcription factor) in CYP19A1 promoters may impair follicular steroidogenesis, leading to poor Oocyte and embryo condition in endometriosis patients.

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“…Endometriosis is known as the presence of endometrial tissues inside an abnormal anatomical location other than those of the uterus. It is a common, estrogen-dependent gynecological disorder that can lead to multiple manifestations, including dysmenorrhea, pelvic pain, pelvic mass, infertility and malignant behaviors [1][2][3][4], with prevalence rates of 10-15% in women of reproductive age [5,6].…”

Section: Introductionmentioning

confidence: 99%

Network-based bioinformatics analysis of HOX genes and their related genes in endometriosis

Chitsazian

Favaedi

Jahromi

et al. 2020

Preprint

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Background: Dysregulation of some HOX genes are important for the development of endometriosis. Endometriosis is a complex gynecologic disorder affecting as many as 10-15% of premenopausal women. The pathogenesis of endometriosis, as the presence of endometrium-like tissue outside the uterine cavity, is largely unknown. HOX genes, encoding homeodomain transcription factors, are dynamically expressed in endometrium, in which they are necessary for endometrial growth, differentiation, and implantation. Few investigations have been studied in this vast range of HOX genes with a network-based view. Network theory allows for a holistic understanding of the role of genes in diseases. The purpose of this study is to identify genes that cause this disease by focusing on the HOX family genes and their regulatory genes. Materials & Methods: Fifteen samples of eutopic and ectopic endometrium collected from patients in proliferation phase. PCR array was performed on sample groups for 84 HOX genes and their related genes. The statistical significance was determined by using t-test. PCA and Hierarchical clustering were carried out between different groups. The genes co-expression networks were constructed based on correlation between normalized gene expression data. Gene function was annotated based on Gene Ontology, the intervention in other diseases and expression in other tissues using the DAVID to clarify the function of important genes and the mechanism of endometriosis. Results: Among the analyzed genes, 30, 40 and 35 significantly differentially expressed genes were in eutopic, ectopic compared with normal and ectopic with eutopic samples, respectively. Twenty-eight genes had no significant alteration in none of the sample groups. Conclusion: The most significant genes and the genes in co-expression networks are effective genes in development, metabolic and neural processes. Another important point is that the some non-differently expressed genes in networks have many interactions with the significant genes, indicating that these non-significant genes also are likely contributed in endometriosis. Keywords: endometriosis, HOX, PCR-array, Co-expression network

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Macrophage migration inhibitory factor as a potential biomarker of endometriosis

Cited by 24 publications

References 28 publications

Serum galectin-9 as a noninvasive biomarker for the detection of endometriosis and pelvic pain or infertility-related gynecologic disorders

Serum galectin-9 as a noninvasive biomarker for the detection of endometriosis and pelvic pain or infertility-related gynecologic disorders

Epigenetic alterations of CYP19A1 gene in Cumulus cells and its relevance to infertility in endometriosis

Network-based bioinformatics analysis of HOX genes and their related genes in endometriosis

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