Macrophage Migration as an Index of Immune Status

Abstract

Peritoneal macrophages from mice and guinea pigs pretreated with Freund's complete adjuvant (FCA) or any other immunostimulant when packed in a glass capillary and placed in a migration chamber migrated to a larger area than macrophages from normal untreated animals. The extent of migration could be correlated with the dose of FCA and the period of treatment. Under optimum conditions the ratio between the areas of migration of macrophages from FCA-treated animals and of macrophages from untreated animals was above 3.0. A close correlation was observed between macrophage migration and delayed type hypersensitivity (DTH) response in animals sensitized with ovalbumin or sheep red blood cells. The macrophages of immunostimulant-treated animals had relatively higher phagocytic activity. The macrophage migration index (MMI) appears to be a close correlate of macrophage activation and possibly also of the status of cell mediated immune response.

Antigiardial and immunostimulatory effect of Piper longum on giardiasis due to Giardia lamblia

“…Macrophage migration index. The test was carried out according to the method of Saxena et al (1991). Briefly, the peritoneal exudate cells (PEC) were collected in RPMI-medium, washed with the same medium, and suspended in complete RPMI-1640 containing 10% fetal calf serum.…”

Immunostimulant Activity of Peptides Related to Human β-Casein Fragment Gly-Leu-Phe

“…On day 0, four animals from each group were sacrificed and peritoneal exudate cells (PEC) from them were collected for the determination of MI (ref. 19 ). The remaining four animals from each group were injected 1 .…”

“…The tripeptides were examined in vivo for their capacity to alter macrophage migration and humoral response to sheep red blood cells (SRBC) in a mouse model. For this macrophage migration index (MI) and hemagglutinating antibody (HA) titre were examined by the methods described elsewhere 18,19 . In brief, male Swiss mice obtained from the CDRI animal house were divided into groups of 8 each.…”

Apyrogenic synthetic desmuramyldipeptide, LK-409, with immunomodulatory properties

“…The tested substances (LK-409 or MDP) dissolved in RPMI 1640 medium (GIBCO, Scotland) were administered i.p. at a single dose (0.25, 2.5 and 25 lag) per day at 2 p.m. for three consecutive days [25], except in haemolytic plaque assay. On the fourth day at 8 a.m. mice were sacrificed with cervical dislocation, peritoneal cavity washed for macrophage collection and spleens removed for perparation of spleen lymphocytes.…”