Macrophage functions in wound healing

Kloc, Małgorzata; Ghobrial, Rafik M.; Wosik, J.; Lewicka, Aneta; Lewicki, Sławomir; Kubiak, Jacek Z.

doi:10.1002/term.2772

Cited by 55 publications

(58 citation statements)

References 102 publications

(130 reference statements)

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“…The polarization of T helper cells into sub-categories in response to different cytokine environments is well established. This concept has recently been extended to innate immune cells in mammals, notably to macrophages with their M1 pro-inflammatory and M2 pro repair subtypes [134]. It is tempting to speculate that Drosophila plasmatocytes can be polarized toward different functions such as enhanced production of antibacterial peptides or phagocytosis according to different inflammation and metabolic states.…”

Section: Plos Onementioning

confidence: 99%

Comparative RNA-Seq analyses of Drosophila plasmatocytes reveal gene specific signatures in response to clean injury and septic injury

2020

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Drosophila melanogaster's blood cells (hemocytes) play essential roles in wound healing and are involved in clearing microbial infections. Here, we report the transcriptional changes of larval plasmatocytes after clean injury or infection with the Gram-negative bacterium Escherichia coli or the Gram-positive bacterium Staphylococcus aureus compared to hemocytes recovered from unchallenged larvae via RNA-Sequencing. This study reveals 676 differentially expressed genes (DEGs) in hemocytes from clean injury samples compared to unchallenged samples, and 235 and 184 DEGs in E. coli and S. aureus samples respectively compared to clean injury samples. The clean injury samples showed enriched DEGs for immunity, clotting, cytoskeleton, cell migration, hemocyte differentiation, and indicated a metabolic reprogramming to aerobic glycolysis, a well-defined metabolic adaptation observed in mammalian macrophages. Microbial infections trigger significant transcription of immune genes, with significant differences between the E. coli and S. aureus samples suggesting that hemocytes have the ability to engage various programs upon infection. Collectively, our data bring new insights on Drosophila hemocyte function and open the route to post-genomic functional analysis of the cellular immune response.

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Section: Plos Onementioning

confidence: 99%

Comparative RNA-Seq analyses of Drosophila plasmatocytes reveal gene specific signatures in response to clean injury and septic injury

2020

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“…Exerting macrophage depletion during skin healing, except the phase of tissue maturation, contributed to immaturities and postponed the development of vascularized granulation and epithelium or hemorrhage when macrophage was absent initially or intermediately, emphasizing the restore function of it (Lucas et al, ). Macrophages' production of vascular endothelial growth factor (VEGF), transforming growth factor beta (TGF‐β) and platelet‐derived growth factor‐BB (PDGF‐BB), as well as the phenotypes of macrophage, cells being attracted, interaction with other immune cells determine the outcome of angiogenesis (Kloc et al, ). Other strategies that could enhance the development of vessels, such as biologic delivering, pharmaceutical and cell‐based therapies, are depicted by Austin et al (Veith, Henderson, Spencer, Sligar, & Baker, ).…”

Section: Fbr Of Implanted Materialsmentioning

confidence: 99%

Modulation of foreign body reaction and macrophage phenotypes concerning microenvironment

Chu

Liu

Rung

et al. 2019

J Biomedical Materials Res

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The foreign body reaction (FBR) is described as a local chronic inflammation after implantation of biomaterials in which macrophages involved intimately. At the stage of acute inflammation, mast cells release histamine, Interleukin-4 (IL-4) andInterleukin-13 (IL-13), enhancing recruitment, and fusion of macrophages in the following phase. As for chronic intensive inflammation, degradation of biomaterials would be promoted by macrophage-derived foreign body giant cells releasing degradative enzymes, acid and reactive oxygen intermediates. Nevertheless, it could be seen as a breakthrough point for regulating FBR, considering the dominant role of the macrophage in the immune response as exemplified by the decrease of IL-4 and IL-13, stabilizing an appropriate balance between two macrophage phenotypes, selectively suppressing some function of macrophages, and so on. Moreover, the relationship between macrophages polarization and the development of a fibrous capsule, which increase the possibility of implantation failure, will be illustrated later.This review aims at providing readers a comprehensive understanding of FBR and its correlative treatment strategy. K E Y W O R D Sbiomaterials, foreign body reaction, macrophages

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“…In contrast, the VEGF level in the MICH group exhibited abundant expression, along with up to 2.5‐fold higher than that of control groups (Figure 5d), suggesting an enhancement in vascularized granulation tissue formation by the MICH. [ 45 ] In brief, the MICH matrix suppressed inflammatory response as well as enhanced re‐epithelialization and angiogenesis in the burn‐induced progressions, which could be attributed to the collusive effects of MICH. On the one hand, in situ forming MICH promptly closed the damaged tissue, preventing wound infection from bacteria and other pathogens. [ 46 ] Most importantly, the MICH matrix could inhibit the transformation from monocytes to proinflammatory M1 macrophages because of ROS scavenging effects of Fe‐SOD mimics, HA, and VE in the MICH matrix on wound tissue. [ 47 ] As a result, the wound bed treated with MICH experienced a mild inflammatory response in the early phase of wound healing, facilitating transit into the tissue formation stage quickly.…”

Section: Resultsmentioning

confidence: 99%

Injectable Enzyme‐Based Hydrogel Matrix with Precisely Oxidative Stress Defense for Promoting Dermal Repair of Burn Wound

Zhang

Wang

Sun

et al. 2020

Macromolecular Bioscience

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Burn wound healing remains a challenging health problem worldwide due to the lack of efficient and precise therapy. Inherent oxidative stress following burn injury is importantly responsible for prolonged inflammation, fibrotic scar, and multiple organ failure. Herein, a bioinspired antioxidative defense system coupling with in situ forming hydrogel, namely, multiresponsive injectable catechol‐Fe3+ coordination hydrogel (MICH) matrix, is engineered to promote burn‐wound dermal repair by inhibiting tissue oxidative stress. This MICH matrix serves as the special traits of “Fe‐superoxide dismutases,” small molecular antioxidant (vitamin E), and extracellular matrix (ECM) in alleviating cellular oxidative damage, which demonstrates precise scavenging on reactive oxygen species (ROS) of different cellular locations, blocking lipid peroxidation and cell apoptosis. In in vivo burn‐wound treatment, this MICH promptly integrates with injured surrounding tissue to provide hydration microenvironment and physicochemical ECM for burn wounds. Importantly, the MICH matrix suppresses tissue ROS production, reducing the inflammatory response, prompting re‐epithelization and neoangiogenesis during wound healing. Meanwhile, the remodeling skin treated with MICH matrix demonstrates low collagen deposition and normal dermal collagen architecture. Overall, the MICH prevents burn wound progression and enhances skin regeneration, which might be a promising biomaterial for burn‐wound care and other disease therapy induced by oxidative stress.

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Macrophage functions in wound healing

Cited by 55 publications

References 102 publications

Comparative RNA-Seq analyses of Drosophila plasmatocytes reveal gene specific signatures in response to clean injury and septic injury

Comparative RNA-Seq analyses of Drosophila plasmatocytes reveal gene specific signatures in response to clean injury and septic injury

Modulation of foreign body reaction and macrophage phenotypes concerning microenvironment

Injectable Enzyme‐Based Hydrogel Matrix with Precisely Oxidative Stress Defense for Promoting Dermal Repair of Burn Wound

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