Macrophage-derived exosome-mimetic hybrid vesicles for tumor targeted drug delivery

Rayamajhi, Sagar; Nguyen, Tuyen Duong Thanh; Marasini, Ramesh; Aryal, Santosh

doi:10.1016/j.actbio.2019.05.054

Cited by 272 publications

(230 citation statements)

References 66 publications

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“…once every 3 days - Improved loading efficiency of DOX when pH close to PI, of PTX when dissolved in ethanol - Higher affinity towards tumor sites, more robust inhibitory potency on tumor growth and prolonged lifespan of both 133 - RAW 264.7 cell line - Ultracentrifugation Exos (~97.3 nm) EVs loaded with DOX - Tumor volumes reached 60 mm 3 - At DOX dose of 5 mg/kg by i.v. every 3 days for total 18 days - Prolonged circulation time of DOX and better accumulation of DOX at tumor tissue - Highest tumor inhibition efficacy - Decreased other tissue lesions 134 - J774A.1 cell line - Membrane filter extrusion EVs (~139 nm) Hybrid EVs with liposomes and loaded with DOX Unclear - Higher drug release in acidic microenvironment - Higher biocompatibility as a safe delivery system with lower cytotoxicity - Decreased K7M2, 4T1, and NIH/3T3 cell viability 135 Vaccine adjuvant Melanoma - RAW264.7 cell line with γ-IFN - Ultracentrifugation Exos (~50 nm) cytokines such as IL-6, IL-12, and γ-IFN - Tumor volume reached ~50 mm 3 - 10 μg EVs by single subcutaneously at 24 h after injection of vaccine - Elevated apoptosis of melanoma cancer cells - Decreased celluar scar tissues as well as many infiltrating immune cells - Significant inhibitory effects on tumor growth 131 …”

Section: Therapeutic Roles Of Mφ-evs In Diseasesmentioning

confidence: 99%

“…These engineered EVs prolonged the release profile of Dox in vitro, and displayed high tumor targeting ability and excellent tumor inhibitory efficiency in mice with TNBC 133 . Another example is hybridizing EVs from M1-like macrophages with liposomes: the resulting hybrid Mφ-EVs exhibited higher cytotoxicity to multiple types of cancer cells, such as osteosarcoma and breast cancer cells, when loaded with Dox 134 . These studies indicate that Mφ-EVs constitute a promising natural carrier for target drug delivery.…”

Section: Therapeutic Roles Of Mφ-evs In Diseasesmentioning

confidence: 99%

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Macrophage-derived extracellular vesicles: diverse mediators of pathology and therapeutics in multiple diseases

et al. 2020

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Macrophages (Mφ) are primary innate immune cells that exhibit diverse functions in response to different pathogens or stimuli, and they are extensively involved in the pathology of various diseases. Extracellular vesicles (EVs) are small vesicles released by live cells. As vital messengers, macrophage-derived EVs (Mφ-EVs) can transfer multiple types of bioactive molecules from macrophages to recipient cells, modulating the biological function of recipient cells. In recent years, Mφ-EVs have emerged as vital mediators not only in the pathology of multiple diseases such as inflammatory diseases, fibrosis and cancers, but also as mediators of beneficial effects in immunoregulation, cancer therapy, infectious defense, and tissue repair. Although many investigations have been performed to explore the diverse functions of Mφ-EVs in disease pathology and intervention, few studies have comprehensively summarized their detailed biological roles as currently understood. In this review, we briefly introduced an overview of macrophage and EV biology, and primarily focusing on current findings and future perspectives with respect to the pathological and therapeutic effects of Mφ-EVs in various diseases.

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Section: Therapeutic Roles Of Mφ-evs In Diseasesmentioning

confidence: 99%

Section: Therapeutic Roles Of Mφ-evs In Diseasesmentioning

confidence: 99%

Macrophage-derived extracellular vesicles: diverse mediators of pathology and therapeutics in multiple diseases

et al. 2020

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“…Liposomes are self-assembling spherical entities composed of lipid bilayers with an aqueous core. Based on the composition of lipids used and other components such as stabilizers, encapsulated molecules, and ligands on the surface; the size of the liposomal nanoparticles vary from nanometers to micrometers (Gizzatov et al, 2014;Mulder et al, 2004;Rayamajhi, Thanh Nguyen, Marasini, & Aryal, 2019). Depending on the location of Gd-complex in liposomal structure, liposomes-based nanoscale MRI CAs can be classified into three categories (Figure 5a-c).…”

Section: Liposomesmentioning

confidence: 99%

Integration of gadolinium in nanostructure for contrast enhanced‐magnetic resonance imaging

Marasini

Nguyen

Aryal

2019

WIREs Nanomed Nanobiotechnol

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Magnetic resonance imaging (MRI) is a routinely used imaging technique in medical diagnostics, which is further enhanced with the use of contrast agents (CAs). The most commonly used CAs are gadolinium‐based contrast agents (GBCAs), in which gadolinium (Gd) is chelated with organic chelating agents (linear or cyclic). However, the use of GBCA is related to toxic side effect due to the release of free Gd3+ ions from the chelating agents. The repeated use of GBCAs has led to Gd deposition in various major organs including bone, brain, and kidneys. As a result, the use of GBCA has been linked to the development of nephrogenic systemic fibrosis (NSF). Due to the GBCA associated toxicities, some clinically approved GBCAs have been limited or revoked recently. Therefore, there is an urgent need for the development of new strategies to chelate and stabilize Gd3+ ions for contrast enhancement, safety profile, and selective imaging of a pathological site. Toward this endeavor, GBCAs have been engineered using different nanoparticulate systems to improve their stability, biocompatibility, and pharmacokinetics. Throughout this review, some of the important strategies for engineering small molecular Gd3+ chelates into a nanoconstruct is discussed. We focus on the development of GBCAs as liposomes, mesoporous silica nanoparticles (MSNs), polymeric nanocarriers, and plasmonic nanoparticles‐based design strategies to improve safety and contrast enhancement for contrast enhanced‐magnetic resonance imaging (Ce‐MRI). We also discuss the in‐vitro/in‐vivo properties of strategically designed nanoscale MRI CAs, its potentials, and limitations. This article is categorized under: Diagnostic Tools > in vivo Nanodiagnostics and Imaging Diagnostic Tools > Diagnostic Nanodevices Toxicology and Regulatory Issues in Nanomedicine > Toxicology of Nanomaterials

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“…To the best of our knowledge, no groups have examined the applications of hybrid exosome-liposome particles embedded in natural or synthetic hydrogels in vitro or in vivo yet. The only studies that have been done up until now using these hybrid particles, were only using free-standing nanovesicles [ 7 , 8 , 9 , 10 ]. Embedding theses hybrid particles in hydrogels is a very pertinent topic to investigate, since, as mentioned before, it can maximize the advantages of the targeting ability of exosomes and the versatility of liposomes while increasing the presence of these smart particles at the desired site, thus increasing their efficiency and the controlled release of bioactive compounds.…”

Section: Nanovesicles-hydrogels Interactionsmentioning

confidence: 99%

“…However, exosomes have limitations in terms of efficient and reproducible loading with drugs or bioactive agents. To address this issue, while equipping liposomes with smart tissue and cell targeting behavior, many research groups have created hybrid liposome-exosome delivery systems [ 7 , 8 , 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Engineering Smart Targeting Nanovesicles and Their Combination with Hydrogels for Controlled Drug Delivery

et al. 2020

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Smart engineered and naturally derived nanovesicles, capable of targeting specific tissues and cells and delivering bioactive molecules and drugs into them, are becoming important drug delivery systems. Liposomes stand out among different types of self-assembled nanovesicles, because of their amphiphilicity and non-toxic nature. By modifying their surfaces, liposomes can become stimulus-responsive, releasing their cargo on demand. Recently, the recognized role of exosomes in cell-cell communication and their ability to diffuse through tissues to find target cells have led to an increase in their usage as smart delivery systems. Moreover, engineering “smarter” delivery systems can be done by creating hybrid exosome-liposome nanocarriers via membrane fusion. These systems can be loaded in naturally derived hydrogels to achieve sustained and controlled drug delivery. Here, the focus is on evaluating the smart behavior of liposomes and exosomes, the fabrication of hybrid exosome-liposome nanovesicles, and the controlled delivery and routes of administration of a hydrogel matrix for drug delivery systems.

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Macrophage-derived exosome-mimetic hybrid vesicles for tumor targeted drug delivery

Cited by 272 publications

References 66 publications

Macrophage-derived extracellular vesicles: diverse mediators of pathology and therapeutics in multiple diseases

Macrophage-derived extracellular vesicles: diverse mediators of pathology and therapeutics in multiple diseases

Integration of gadolinium in nanostructure for contrast enhanced‐magnetic resonance imaging

Engineering Smart Targeting Nanovesicles and Their Combination with Hydrogels for Controlled Drug Delivery

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