Macrophage-derived chemokine production by activated human T cells in vitro and in vivo: preferential association with the production of type 2 cytokines

Galli, Grazia; Chantry, David; Annunziato, Francesco; Romagnani, Paola; Cosmi, Lorenzo; Lazzeri, Elena; Manetti, Roberto; Maggi, Enrico; Gray, Patrick W.; Romagnani, Sergio

doi:10.1002/1521-4141(200001)30:1<204::aid-immu204>3.3.co;2-7

Cited by 55 publications

(67 citation statements)

References 3 publications

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“…This may not be surprising because JRA does have a strong type 1 component, and increases in MDC serum levels relative to healthy adults have been reported for type 2-dominated disorders such as atopic dermatitis and mycosis fungoides/Sézary syndrome but not for type 1-dominated disorders such as multiple sclerosis or Crohn's disease (31). Although only a relatively small number of samples were studied, two interesting observations are apparent.…”

Section: Discussionmentioning

confidence: 99%

Chemokine Receptor CCR4 on CD4+ T Cells in Juvenile Rheumatoid Arthritis Synovial Fluid Defines a Subset of Cells with Increased IL-4:IFN-γ mRNA Ratios

Thompson

Luyrink

Graham

et al. 2001

The Journal of Immunology

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To understand the mechanisms that promote recruitment and survival of T cells within the pediatric inflamed joint, we have studied the expression of CCR4 and CCR5 on synovial fluid T cells and matched peripheral blood samples from juvenile rheumatoid arthritis (JRA) patients using three-color flow cytometric analysis. Thymus- and activation-regulated chemokine and macrophage-derived chemokine, ligands for CCR4, were measured by ELISA in JRA synovial fluid, JRA plasma, adult rheumatoid arthritis synovial fluid, and normal plasma. IL-4 and IFN-γ mRNA production was assessed in CD4+/CCR4+ and CD4+/CCR4− cell subsets. We found accumulations of both CCR4+ and CCR5+ T cells in JRA synovial fluids and a correlation for increased numbers of CCR4+ T cells in samples collected early in the disease process. Thymus- and activation-regulated chemokine was detected in JRA synovial fluid and plasma samples, but not in adult rheumatoid arthritis synovial fluid or control plasma. Macrophage-derived chemokine was present in all samples. CD4+/CCR4+ synovial lymphocytes produced more IL-4 and less IFN-γ than CD4+/CCR4− cells. These findings suggest that CCR4+ T cells in the JRA joint may function early in disease in an anti-inflammatory capacity through the production of type 2 cytokines and may play a role in determining disease phenotype.

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Section: Discussionmentioning

confidence: 99%

Chemokine Receptor CCR4 on CD4+ T Cells in Juvenile Rheumatoid Arthritis Synovial Fluid Defines a Subset of Cells with Increased IL-4:IFN-γ mRNA Ratios

Thompson

Luyrink

Graham

et al. 2001

The Journal of Immunology

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“…Serum CCL22/MDC levels are also known to be closely related to the disease activity of atopic dermatitis [15] and mycosis fungoides/Sézary Syndrome [14], which are associated with Th2 polarization.…”

Section: Discussionmentioning

confidence: 99%

“…CCR4, the receptor of CCL22/MDC [17] and CCL17/TARC [18], is primarily Activated Th2 cells produce CCL22/MDC [14], IL-4, and IL-13. IL-4 and IL-13 are able to amplify CCL22/MDC production from Th2 cells [13] and monocytes [19].…”

Section: Discussionmentioning

confidence: 99%

The role of CCL22/macrophage-derived chemokine in allergic rhinitis

Yanai

Sato

Aoki

et al. 2007

Clinical Immunology

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Dendritic Cells (DCs) are considered to be the most powerful antigen-presenting cells (APCs). DCs are thought to be associated with Th1 or Th2 polarization and with polarization-induced disease such as atopic dermatitis, asthma and allergic rhinitis, but its mechanism is not well known. In this study, we analyzed the mRNA expression of DCs between birch pollen allergic rhinitis and healthy controls by using cDNA array.We found that the expressions of CCL22/macrophage derived chemokine (MDC) differed significantly. We also revealed that CCL22/MDC production was higher in patients than in healthy donors. By chemotaxis assay, CCL22/MDC can enhance the migration of patient's T cells rather than those of healthy controls. Surface marker analysis of migrated cells revealed that the most of migrated cells expressed CCR4, which were considered to be Th2 cells. Furthermore, CD1a + CD83 + cells located in the nasal mucosa expressed CCL22/MDC in vivo. To the best of our knowledge, this is the first report clearly indicating the role of CCL22/MDC in allergic rhinitis.

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“…It is probable that, as for basophils, TH2-type mRNA ϩ cells may be recruited in vivo through additional chemokine receptors, such as CCR4 and CCR8, expressed on the latter cells (Bonecchi et al, 1998). In this context, it has been reported that in human allergic cutaneous reactions, there is an overexpression of thymus activation regulated chemokine (CCL17) (Kakinuma et al, 2001;Vestergaard et al, 2000) and macrophage-derived chemokine (CCL22) (Galli et al, 2000), which bind CCR4 (Imai et al, 1997(Imai et al, , 1998. The in vivo relevance of the CCR4 pathway on TH2 cell recruitment in allergic inflammation has been assessed indirectly using antibody against macrophage-derived chemokine in a murine model, where it was mainly effective on the chronic recruitment of TH2 cells (Lloyd et al, 2000).…”

Section: Sénéchal Et Almentioning

confidence: 99%

CCR3-Blocking Antibody Inhibits Allergen-Induced Eosinophil Recruitment in Human Skin Xenografts from Allergic Patients

Sénéchal

Fahy

Gentina

et al. 2002

Laboratory Investigation

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SUMMARY:Eosinophil, basophil, and T helper 2 (TH2) cell recruitment into tissues is a characteristic feature of allergic diseases.These cells have in common the expression of the chemokine receptor CCR3, which may represent a specific pathway for their accumulation in vivo. Although animal models of allergic reactions are available, findings cannot always be extrapolated to man. To overcome these limitations, we have developed a humanized mouse model of allergic cutaneous reaction using severe combined immunodeficiency mice engrafted with skin and autologous peripheral blood mononuclear cells from allergic donors. Intradermal injection of the relevant allergen into human skin xenografts from allergic individuals induced a significant recruitment of human CD4 ϩ T cells, basophils, and TH2-type cytokine mRNA-expressing cells, as well as murine eosinophils. Human skin xenografts, atopic status, and autologous peripheral blood mononuclear cell reconstitution were all mandatory to induce the allergic reaction. Next, we addressed the role of CCR3 in the endogenous mechanisms involved in the inflammatory cell recruitment in this experimental model of allergic cutaneous reaction. In vivo administration of an anti-human CCR3-blocking antibody selectively reduced accumulation of eosinophils but not that of CD4 ϩ cells, basophils, or cells expressing mRNA for TH2-type cytokines. These findings establish a new in vivo model of humanized allergic reaction and suggest that eosinophil migration is mediated mainly through CCR3. Finally, these results suggest that this model might be useful to test human-specific antiallergic modulators. (Lab Invest 2002, 82:929 -939).A llergic diseases are characterized by a tissue infiltration of eosinophils, basophils and T helper 2 (TH2) cells, which is thought to be related to the severity and chronicity of the allergic reaction. This preferential cell accumulation in tissue suggests that there are specific pathways used for their accumulation in vivo. Understanding these mechanisms could aid in developing pharmacologic therapies that would block their recruitment. This recruitment is orchestrated in part by chemokines that act through specialized surface receptors. The chemokine receptor CCR3 is of particular interest in the context of allergic reactions, because it is expressed by TH2 lymphocytes (Sallusto et al, 1997), eosinophils (Ponath et al, 1996), basophils (Uguccioni et al, 1997), and mast cells (Ochi et al, 1999;Romagnani et al, 1999), all of which are found at sites of allergen-induced latephase response (LPR) (Frew and Kay, 1988;Gaga et al, 1991;Irani et al, 1998). The CCR3 receptor is a Gprotein-coupled seven-transmembrane receptor that mediates the action of several chemokines, including eotaxin (CCL11), RANTES (CCL5), MCP-3 (CCL7), and MCP-4 (CCL13) (Ponath et al, 1996;Stellato et al, 1997). All these ligands have been shown to be overexpressed in human allergen-induced cutaneous LPR (Ying et al, 1999), in atopic dermatitis (Yawalkar et al, 1999) and in asthma (Lamkhioued et ...

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Macrophage-derived chemokine production by activated human T cells in vitro and in vivo: preferential association with the production of type 2 cytokines

Cited by 55 publications

References 3 publications

Chemokine Receptor CCR4 on CD4+ T Cells in Juvenile Rheumatoid Arthritis Synovial Fluid Defines a Subset of Cells with Increased IL-4:IFN-γ mRNA Ratios

Chemokine Receptor CCR4 on CD4+ T Cells in Juvenile Rheumatoid Arthritis Synovial Fluid Defines a Subset of Cells with Increased IL-4:IFN-γ mRNA Ratios

The role of CCL22/macrophage-derived chemokine in allergic rhinitis

CCR3-Blocking Antibody Inhibits Allergen-Induced Eosinophil Recruitment in Human Skin Xenografts from Allergic Patients

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