Macrophage-Dependent Trafficking and Remodeling of the Basement Membrane-Interstitial Matrix Interface

Bahr, Julian; Weiss, Stephen J.

doi:10.1101/364422

Cited by 6 publications

(6 citation statements)

References 94 publications

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“…Collagen IV is a constituent of basement membranes and is indispensable for the structural integrity and functions of these specialized, self-assembled extracellular matrices [39]. It was shown that macrophages directly interact with the developing vasculature during angiogenesis [40] and might remodel native basement membrane barriers in a MMP14-dependent fashion [41]. Our results indicate that macrophages are also a source of collagen IV and might contribute to the integrity of vascular and skin basement membranes.…”

Section: Discussionmentioning

confidence: 58%

Gene Expression Profiling of the Extracellular Matrix Signature in Macrophages of Different Activation Status: Relevance for Skin Wound Healing

Etich

Koch

Wagener

et al. 2019

IJMS

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The extracellular matrix (ECM) provides structural support for tissue architecture and is a major effector of cell behavior during skin repair and inflammation. Macrophages are involved in all stages of skin repair but only limited knowledge exists about macrophage-specific expression and regulation of ECM components. In this study, we used transcriptome profiling and bioinformatic analysis to define the unique expression of ECM-associated genes in cultured macrophages. Characterization of the matrisome revealed that most genes were constitutively expressed and that several genes were uniquely regulated upon interferon gamma (IFNγ) and dexamethasone stimulation. Among those core matrisome and matrisome-associated components transforming growth factor beta (TGFβ)-induced, matrix metalloproteinase 9 (MMP9), elastin microfibril interfacer (EMILIN)-1, netrin-1 and gliomedin were also present within the wound bed at time points that are characterized by profound macrophage infiltration. Hence, macrophages are a source of ECM components in vitro as well as during skin wound healing, and identification of these matrisome components is a first step to understand the role and therapeutic value of ECM components in macrophages and during wound healing.

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Section: Discussionmentioning

confidence: 58%

Gene Expression Profiling of the Extracellular Matrix Signature in Macrophages of Different Activation Status: Relevance for Skin Wound Healing

Etich

Koch

Wagener

et al. 2019

IJMS

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“…Related to this, a report revealed that MMP-1 in the melanoma is engaged in a proteolytic cleavage that generates active TGF-β from its latent form [175]. Finally, TAMs-derived MMP-14, a collagenase also known as membrane type-1 (MT1)-MMP, is actively involved in breaking the surrounding basement membrane [176]. These mechanisms are all integrated into one context to enhance the individual or collective forms of invasive cancer cells invasions.…”

Section: Tumor-associated Ecm: Another Side Of the Micro-invasive Nichementioning

confidence: 96%

“…First, the basement membrane of the endothelial layer, supported by pericytes and second the intact ECs [193]. This basement membrane has the same composition as the epithelial-basement membrane and is degraded with the proteolytic function of cancer cells invadopodia and macrophage-derived MMPs, notably MT1-MMP [176,181]. Indeed, more effort is required to remove the second barrier since in normalized blood vessels ECs are in close contact at the VE-cadherin and ZO-1 junctions [194].…”

Section: Intravasation and Struggle To Survivementioning

confidence: 99%

Pro-tumorigenic functions of macrophages at the primary, invasive and metastatic tumor site

Nasrollahzadeh

Razi

Keshavarz-Fathi

et al. 2020

Cancer Immunol Immunother

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The tumor microenvironment (TME) not only facilitates cancer progression from the early formation to distant metastasis, but also it differs itself from time to time alongside the tumor evolution. Tumor-associated macrophages (TAMs), whether as pre-existing tissue-resident macrophages or recruited monocytes, are an inseparable part of this microenvironment. As their parents are broadly classified into a dichotomic, simplistic M1 and M2 subtypes, TAMs also exert paradoxical and diverse phenotypes as they are settled in different regions of TME and receive different microenvironmental signals. Briefly, M1 macrophages induce an inflammatory precancerous niche and flame the early oncogenic mutations, whereas their M2 counterparts are reprogrammed to release various growth factors and providing an immunosuppressive state in TME as long as abetting hypoxic cancer cells to set up a new vasculature. Further, they mediate stromal micro-invasion and co-migrate with invasive cancer cells to invade the vascular wall and neural sheath, while another subtype of TAMs prepares suitable niches much earlier than metastatic cells arrive at the target tissues. Accordingly, at the neoplastic transformation, during the benign-to-malignant transition and through the metastatic cascade, macrophages are involved in shaping the primary, micro-invasive and pre-metastatic TMEs. Whether their behavioral plasticity is derived from distinct genotypes or is fueled by microenvironmental cues, it could define these cells as remarkably interesting therapeutic targets.

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“…These results demonstrate once again the influence of the state and composition of the ECM on cell adhesion. On the other hand, collagen IV is an indispensable constituent for the structural integrity of basement membranes [ 49 ] and it was shown that macrophages directly might remodel these native barriers [ 50 ]. Moreover, results published by Etich et al indicate that macrophages are also a source of collagen IV and might contribute to the integrity of vascular and skin basement membranes [ 51 ].…”

Section: Resultsmentioning

confidence: 99%

Candida albicans/Macrophage Biointerface on Human and Porcine Decellularized Adipose Matrices

Cicuéndez

Casarrubios

Feito

et al. 2021

JoF

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Macrophages, cells effective in sensing, internalizing and killing Candida albicans, are intertwined with the extracellular matrix (ECM) through different signals, which include the release of specific cytokines. Due to the importance of these interactions, the employment of in vitro models mimicking a fungal infection scenario is essential to evaluate the ECM effects on the macrophage response. In this work, we have analyzed the effects of human and porcine decellularized adipose matrices (DAMs), obtained by either enzymatic or organic solvent treatment, on the macrophage/Candida albicans interface. The present study has allowed us to detect differences on the activation of macrophages cultured on either human- or porcine-derived DAMs, evidencing changes in the macrophage actin cytoskeleton, such as distinct F-actin-rich membrane structures to surround the pathogen. The macrophage morphological changes observed on these four DAMs are key to understand the defense capability of these cells against this fungal pathogen. This work has contributed to the knowledge of the influence that the extracellular matrix and its components can exert on macrophage metabolism, immunocompetence and capacity to respond to the microenvironment in a possible infection scenario.

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Macrophage-Dependent Trafficking and Remodeling of the Basement Membrane-Interstitial Matrix Interface

Cited by 6 publications

References 94 publications

Gene Expression Profiling of the Extracellular Matrix Signature in Macrophages of Different Activation Status: Relevance for Skin Wound Healing

Gene Expression Profiling of the Extracellular Matrix Signature in Macrophages of Different Activation Status: Relevance for Skin Wound Healing

Pro-tumorigenic functions of macrophages at the primary, invasive and metastatic tumor site

Candida albicans/Macrophage Biointerface on Human and Porcine Decellularized Adipose Matrices

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