Macrophage colony-stimulating factor (CSF1) controls monocyte production and maturation and the steady-state size of the liver in pigs

Sauter, Kristin A.; Waddell, Lindsey; Lisowski, Zofia M.; Young, Rachel; Lefèvre, Lucas; Davis, Gemma M.; Clohisey, Sara; McCulloch, Mary B.; Magowan, Elizabeth; Mabbott, Neil A.; Summers, Kim M.; Hume, David

doi:10.1152/ajpgi.00116.2016

Cited by 50 publications

(64 citation statements)

References 51 publications

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“…In principle, if there was significant heterogeneity in metabolic state or development among the liver samples, a gene-to-gene clustering might reveal sets of genes associated with portal versus centrilobular regions of liver lobules. Centrilobular genes were found to be downregulated in liver of pigs treated with macrophage colony-stimulating factor (CSF1) (Sauter et al, 2016) as also seen in regenerating liver in other species. Table 2 shows the GO term enrichment for the largest clusters identified using the liver samples, generated at Pearson correlation threshold of 0.75 ( Figure S2B) and Table S3 contains lists of genes within these clusters.…”

Section: The Transcriptome Of the Pig Livermentioning

confidence: 93%

Functional Annotation of the Transcriptome of the Pig, Sus scrofa, Based Upon Network Analysis of an RNAseq Transcriptional Atlas

Summers

Bush

et al. 2020

Front. Genet.

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The domestic pig (Sus scrofa) is both an economically important livestock species and a model for biomedical research. Two highly contiguous pig reference genomes have recently been released. To support functional annotation of the pig genomes and comparative analysis with large human transcriptomic data sets, we aimed to create a pig gene expression atlas. To achieve this objective, we extended a previous approach developed for the chicken. We downloaded RNAseq data sets from public repositories, down-sampled to a common depth, and quantified expression against a reference transcriptome using the mRNA quantitation tool, Kallisto. We then used the network analysis tool Graphia to identify clusters of transcripts that were coexpressed across the merged data set. Consistent with the principle of guilt-by-association, we identified coexpression clusters that were highly tissue or cell-type restricted and contained transcription factors that have previously been implicated in lineage determination. Other clusters were enriched for transcripts associated with biological processes, such as the cell cycle and oxidative phosphorylation. The same approach was used to identify coexpression clusters within RNAseq data from multiple individual liver and brain samples, highlighting cell type, process, and region-specific gene expression. Evidence of conserved expression can add confidence to assignment of orthology between pig and human genes. Many transcripts currently identified as novel genes with ENSSSCG or LOC IDs were found to be coexpressed with annotated neighbouring transcripts in the same orientation, indicating they may be products of the same transcriptional unit. The metaanalytic approach to utilising public RNAseq data is extendable to include new data sets and new species and provides a framework to support the Functional Annotation of Animals Genomes (FAANG) initiative.

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Section: The Transcriptome Of the Pig Livermentioning

confidence: 93%

Functional Annotation of the Transcriptome of the Pig, Sus scrofa, Based Upon Network Analysis of an RNAseq Transcriptional Atlas

Summers

Bush

et al. 2020

Front. Genet.

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“…As possible hepatic signals that recruit macrophages into the liver during systemic inflammation, we examined whether the interleukin-34 (IL-34) and colony stimulating factor-1 (CSF-1) that share a common receptor CSF1R had a role. IL-34 and CSF-1 are known to mediate various functions of macrophages including inflammatory processes and promoting production of pro-inflammatory chemokines [50,54,55]. They have recently been shown to be required for macrophage migration and colonization of the brain to form microglia in zebrafish [56][57][58].…”

Section: Macrophage Recruitment To the Liver Is A Myd88-dependent Infmentioning

confidence: 99%

Drainage of inflammatory macromolecules from brain to periphery targets the liver for macrophage infiltration

Jiménez

Earley

Hamlin

et al. 2020

Preprint

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Many brain pathologies are associated with liver damage, but a direct link has long remained elusive. Here, we establish a new paradigm for interrogating brain-periphery interactions by leveraging zebrafish for its unparalleled access to the intact whole animal for in vivo analysis in real time after triggering focal brain inflammation. We reveal that drainage of inflammatory macromolecules from the brain led to a strikingly robust peripheral infiltration of macrophages into the liver independent of Kupffer cells. We further demonstrate that this macrophage recruitment requires signaling from the cytokine IL-34, Toll-like receptor adaptor protein MyD88, and neutrophils. These results highlight the ability for circulation of brain-derived substances to serve as a rapid mode of communication from brain to the liver. Understanding how the brain engages the periphery at times of danger may offer new perspectives for detecting and treating brain pathologies.

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“…With extensive proliferation of hepatocytes higher infiltration of immune surveillance cells such as macrophages [58] takes place to attack damaged cells. Macrophage infiltration plays important role in liver regeneration [59] ( Table 2). BM-MSCs decreased liver fibrosis and contributed to an increase in oval cells, generation of new hepatocytes and/or to the improvement of resident hepatocytes [43] (Figure 1).…”

Section: Mechanisms Of Liver Regenerationmentioning

confidence: 99%

“…showing use of bone marrow derived SEPCs in proliferation of hepatocytes after engraftment in morbid liver. Macrophages, natural killer (NK) cells, natural killer T (NKT) cells, dendritic cells (DC), eosinophils, gamma delta T (γδT) cells, and conventional T cells, as well as other subsets of the immune cells residing in the liver control liver regeneration [59] (Table 2). Few signaling pathways are important for liver regeneration.…”

Section: Hepatocytesmentioning

confidence: 99%

Stem Cell Therapeutics of Acute Liver Diseases, Transplantation, and Regeneration

Upadhyay¹

2017

J Cell Sci Ther

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Present review article explains various causes of acute liver diseases and their therapeutics. This article describes major reasons of hepatic pathophysiological conditions and diseases including hepatitis, cholestasis, alcoholic and non-alcoholic steatohepatitis, jaundice, liver cirrhosis, carcinogenesis and many others. This article emphasizes use of proliferating hepatocytes, hepatic oval cells, adult human liver mesenchymal stem/progenitor cells, induced pluripotent stem cells (iPSCs) and hematopoietic stem cells in cell transplantation for restoration of liver structure and function. It also justified the therapeutic role of cell secreted growth factors and dietary factors required during natural healing and regeneration liver after surgery or liver transplantation. It sketches out regulatory roles of signaling pathways, expression of cell cycle regulators, growth factors, cytokines, and role of different mitogens in induction of liver stem/progenitor cells (LSPCs) after organ/stem cell transplantation. This article suggests a need for development of new advanced biomaterials, methods, technologies and stem cells for development of targeted therapies to combat and cure liver related diseases and disorders. This article also advice people for avoiding excessive use of alcohol, drugs, fats, salt, high energy diets, and iron as all are responsible of liver cirrhosis, damage and failure.

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Macrophage colony-stimulating factor (CSF1) controls monocyte production and maturation and the steady-state size of the liver in pigs

Cited by 50 publications

References 51 publications

Functional Annotation of the Transcriptome of the Pig, Sus scrofa, Based Upon Network Analysis of an RNAseq Transcriptional Atlas

Functional Annotation of the Transcriptome of the Pig, Sus scrofa, Based Upon Network Analysis of an RNAseq Transcriptional Atlas

Drainage of inflammatory macromolecules from brain to periphery targets the liver for macrophage infiltration

Stem Cell Therapeutics of Acute Liver Diseases, Transplantation, and Regeneration

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