Macrophage Cell Membrane Camouflaged Au Nanoshells for in Vivo Prolonged Circulation Life and Enhanced Cancer Photothermal Therapy

Abstract

Macrophage cell membrane (MPCM)-camouflaged gold nanoshells (AuNS) that can serve as a new generation of photothermal conversion agents for in vivo photothermal cancer therapy are presented. They are constructed by the fusion of biocompatible AuNSs and MPCM vesicles. The resulting MPCM-coated AuNSs exhibited good colloidal stability and kept the original near-infrared (NIR) adsorption of AuNSs. Because AuNS carried high-density coverage of MPCMs, the totally functional portions of macrophage cells membrane were grafted onto the surface of AuNSs. This surface functionalization provided active targeting ability by recognizing tumor endothelium and thus improved tumoritropic accumulation compared to the red blood cell membrane-coating approach. These biomimetic nanoparticles significantly enhance in vivo blood circulation time and local accumulation at the tumor when administered systematically. Upon NIR laser irradiation, local heat generated by the MPCM-coated AuNS achieves high efficiency to suppress tumor growth and selectively ablate cancerous cells within the illuminated zone. Therefore, MPCM-coated AuNSs remained the natural properties of their source cells, which may improve the efficacy of photothermal therapy modulated by AuNSs and other noble-metal nanoparticles.

Human cytotoxic T-lymphocyte membrane-camouflaged nanoparticles combined with low-dose irradiation: a new approach to enhance drug targeting in gastric cancer

“…38 In recent years, it has been shown that leukocytes are effective in carrying drugs and nanoparticles for cancer therapy, 39,40 while leukocyte cell membrane-camouflaged nanoparticles preserve the surface properties of parent leukocytes and provide a platform for biomimetic delivery. 22,[41][42][43] ICAM-1 is a ligand for LFA-1, which is found on vascular endothelium, squamous cell …”

Nanomedicines for dysfunctional macrophage-associated diseases

“…The MPCM-coated AuNS were applied for photothermal cancer therapy. Upon laser irradiation, local heat generated by the MPCM-coated AuNS achieved sufficiently high efficiency to suppress tumor growth and selectively ablated cancerous cells [140]. …”

Synthesis, Functionalization, and Design of Magnetic Nanoparticles for Theranostic Applications

“…[438] In fact, derived and purified cellular membrane from a cell source is integrated with the surface of nanoparticles to somehow "camouflage" them. Several reports have proved significant MPS evasion and tumor accumulation of nanoparticles through coating them with the membranes of erythrocytes, [439] leukocytes, [440] platelets, [441] and macrophage cells [442] as well as "self" peptides such CD47. [443] A simple sonication method can be applied to fully coat nanoparticles by exploiting the asymmetric charge of the cell membranes.…”