Macrophage CD74 contributes to MIF-induced pulmonary inflammation

Takahashi, Koichiro; Koga, Kayoko; Linge, Helena M.; Zhang, Yinzhong; Lin, Xinchun; Metz, Christine N.; Al‐Abed, Yousef; Ojamaa, Kaie; Miller, Edmund J.

doi:10.1186/1465-9921-10-33

Cited by 112 publications

(97 citation statements)

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“…Myosin light-chain kinase, a central cytoskeletal regulator encoded by Mylk, has a key pathophysiological role in ALI (26); and v) Mif, macrophage migration inhibitory factor, is involved in the pathogenesis of several inflammatory diseases. Mif-induced neutrophils accumulate in the alveolar space, indicating that Mif may be a useful target in the reduction of neutrophil lung inflammation and ALI (27). These methylated genes are involved in important mechanisms that underlie ALI/ARDS.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide analysis of DNA methylation in rat lungs with lipopolysaccharide-induced acute lung injury

Zhang

Liu

et al. 2013

Molecular Medicine Reports

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Abstract. Acute lung injury and acute respiratory distress syndrome (ALI/ARDS) are associated with high morbidity and mortality in patients, however, the precise pathogenesis of ALI/ARDS remains unknown. Lipopolysaccharide (LPS) exhibits a number of critical functions and may be associated with the DNA methylation of genes in the lungs. In the present study a genome-wide analysis of DNA methylation was performed in rat lungs with LPS-induced ALI/ARDS. Normal and LPS-induced lung tissues with ALI were analyzed using methylated DNA immunoprecipitation and a rat DNA methylation promoter plus CpG island microarray and the candidate genes were validated by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). Aberrant DNA methylation of the promoter regions of 1,721 genes and the CpG islands of 990 genes was identified when normal lung tissues and lung tissues with LPS-induced ALI/ARDS were compared. These genes were commonly located on chromosomes 1, 3, 5, 7 and 10 (P<0.01). Methylation level and CpG density were compared and it was found that genes associated with high CpG density promoters had a high ratio of methylation. Furthermore, we performed gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. In addition, three genes (Mapk3, Pak1 and Rac2) were validated in the control and lung tissues with ALI by RT-PCR. The results indicate that aberrant DNA methylation of lung tissues may be involved in the pathophysiology of LPS-induced ALI/ARDS. Future studies are required to evaluate the therapeutic and prognostic value of the current novel observations in ALI/ARDS.

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Section: Discussionmentioning

confidence: 99%

Genome-wide analysis of DNA methylation in rat lungs with lipopolysaccharide-induced acute lung injury

Zhang

Liu

et al. 2013

Molecular Medicine Reports

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“…ICAM-1 is involved in neutrophil chemotaxis by binding with the surface ligand CD11/CD18 on neutrophils and lymphocytes (Dominguez et al, 2015;Liu et al, 2015;Sumagin and Parkos, 2015). MIP-2 belongs to the chemokine C-X-C subfamily and induces marked chemotaxis in neutrophils and lymphocytes (Takahashi et al, 2009;Tateno et al, 2013;Vagima et al, 2015). ALI occurrence was closely associated with neutrophil and lymphocyte accumulation in lung tissue.…”

Section: Discussionmentioning

confidence: 99%

Protective effect of quercetin on acute lung injury in rats with sepsis and its influence on ICAM-1 and MIP-2 expression

Meng

et al. 2016

Genet. Mol. Res.

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ABSTRACT. This study aimed to explore the protective effect of quercetin on acute lung injury (ALI) in rats with sepsis and the related mechanism. Rats were administered different doses of quercetin intraperitoneally, and blood samples and lung tissue were collected at 24 h after treatment. Arterial blood gases, lung water content, protein content, and cell counts in bronchoalveolar lavage fluid (BALF) were measured. Morphological changes in lung tissue pathology were observed under a light microscope. Serum intercellular adhesion molecule (ICAM)-1 and macrophage inflammatory protein 2 (MIP-2) levels were detected and ICAM-1 and MIP-2 mRNA expression in lung tissue was determined. Compared with that in the control model group, arterial blood gases, lung water content, protein content, and cell counts in BALF improved in the high-and low-dose quercetin groups (P < 0.05), with maximal improvement observed for the highdose quercetin (P < 0.05). Lesions on the lungs improved in the high- and low-dose quercetin groups than those in the control model group, and the high-dose quercetin group showed better improvement than the low-dose group (P < 0.05). Compared with that in the sham-operated group, both serum and lung tissue ICAM-1 and MIP-2 expression increased significantly in the model group (P < 0.05). The quercetin groups presented lower ICAM-1 and MIP-2 expression than the control model group, with the lowest expression observed in the high-dose group (P < 0.05). Quercetin may protect against ALI in rats with sepsis by inhibiting ICAM-1 and MIP-2 expression.

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“…6,7 Currently only a few numbers of MWSMs with limited wavelengths are known and commercially available to date.…”

Section: 3mentioning

confidence: 99%

“…4,5 However, increasing use of fluorescent molecules from a wide range of emission (em) wavelengths (400 to 700 nm) needs MWSM with a broader fluorescence spectrum. 6,7 Currently only a few numbers of MWSMs with limited wavelengths are known and commercially available to date. 8 For example, GE Healthcare's latest MWSM of dual indication with visible and fluorescent dyes is useful for proteomics analysis, but the use of Cy3 and Cy5 dyes limits it detection range.…”

Section: 3mentioning

confidence: 99%

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Wide-Ranged Fluorescent Molecular Weight Size Markers for Electrophoresis

Park

Kim

Lee

et al. 2013

Bulletin of the Korean Chemical Society

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A molecular weight size marker (MWSM) is commonly used in gel electrophoresis for protein and nucleic acid analyses.1 In electrophoresis, biomolecules are detected by a wide variety of labels such as direct tagging, antibody, and silver stain, often with a set of protein or DNA standards for molecular weight based visual identification of analytes. 2,3Development of enhanced MWSMs has been made to ease the size indications, and visible and fluorescent rainbow MWSMs have been well-acknowledged for the convenience of color-based size indications to overcome limitations and difficulties in use of identically stained markers (GE healthcare and Invitrogen, US).4,5 However, increasing use of fluorescent molecules from a wide range of emission (em) wavelengths (400 to 700 nm) needs MWSM with a broader fluorescence spectrum. 6,7 Currently only a few numbers of MWSMs with limited wavelengths are known and commercially available to date.8 For example, GE Healthcare's latest MWSM of dual indication with visible and fluorescent dyes is useful for proteomics analysis, but the use of Cy3 and Cy5 dyes limits it detection range. 4,5,9Here in, we report our effort to develop a wide ranged fluorescent MWSM, which covers upto near-infrared wavelengths, consisted of sequentially arranged cyanine dyes enabling proteomic analysis without post-stain for size indication. The development of new MWSM system that can cover all range of the fluorescent wavelength is an imperative task. Previously reported cyanine dyes equipped with vinyl sulfone linker were utilized in this work due to their optimal stability and robustness for protein conjugation.10 The absorption (abs) wavelength of FPG-456 is 495 nm and its em wavelength is 522 nm, and those of FPR-553 and FPR-648 are 551 nm/570 nm and 648 nm/672 nm (abs/em), respectively. Combination of three dyes can create a range of emission spectra from 450 to 700 nm. Commonly used six standard proteins, 11 phosphorylase b (97.6 kDa), albumin (66 kDa), glyceraldehyde-3-phophate dehydrogenase (35 kDa), carbonic anhydrase (29 kDa), trypsin inhibitor (20 kDa), and lysozyme (14 kDa) were used as size indicating markers (Aldrich, US). The conjugation between a protein and a dye was precisely followed previously reported protocol.10 In brief, 1.0 eq of given protein and 1.0 eq of vinyl sulfone dye were incubated in aqueous pH 8.0 phosphate or carbonate buffer (0.1 M) at 36 ºC for 4 h to afford corresponding protein-dye complex which was stored and used in the analyses without purification. From the product solution, the dye-conjugated protein solution (1 µL) was diluted to 200 folds in PBS.Optical properties of products were measured with a fluorescence plate reader with a monochromator (EnSpire, Perkin-Elmer, Germany).

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Macrophage CD74 contributes to MIF-induced pulmonary inflammation

Cited by 112 publications

References 50 publications

Genome-wide analysis of DNA methylation in rat lungs with lipopolysaccharide-induced acute lung injury

Genome-wide analysis of DNA methylation in rat lungs with lipopolysaccharide-induced acute lung injury

Protective effect of quercetin on acute lung injury in rats with sepsis and its influence on ICAM-1 and MIP-2 expression

Wide-Ranged Fluorescent Molecular Weight Size Markers for Electrophoresis

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