Macrophage Biocompatibility of CoCr Wear Particles Produced under Polarization in Physiological Hyaluronic Acid Solution

Pérez-Maceda, B. T.; López-Fernández, María Encarnación; Díaz, Iván; Kavanaugh, Aaron; Billi, Fabrizio; Escudero, M.L.; García‐Alonso, M. C.; Lozano, Rosa M.

doi:10.20944/preprints201803.0126.v1

2018

DOI: 10.20944/preprints201803.0126.v1

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Macrophage Biocompatibility of CoCr Wear Particles Produced under Polarization in Physiological Hyaluronic Acid Solution

B. T. Pérez-Maceda¹,

María Encarnación López-Fernández²,

Iván Díaz³

et al.

Abstract: ABSTRACT

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Cited by 4 publications

References 31 publications

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Improvement of CoCr Alloy Characteristics by Ti-Based Carbonitride Coatings Used in Orthopedic Applications

et al. 2020

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The response of the human body to implanted biomaterials involves several complex reactions. The potential success of implantation depends on the knowledge of the interaction between the biomaterials and the corrosive environment prior to the implantation. Thus, in the present study, the in vitro corrosion behavior of biocompatible carbonitride-based coatings are discussed, based on microstructure, mechanical properties, roughness and morphology. TiCN and TiSiCN coatings were prepared by the cathodic arc deposition method and were analyzed as a possible solution for load bearing implants. It was found that both coatings have an almost stoichiometric structure, being solid solutions, which consist of a mixture of TiC and TiN, with a face-centered cubic (FCC) structure. The crystallite size decreased with the addition of Si into the TiCN matrix: the crystallite size of TiCN was 16.4 nm, while TiSiCN was 14.6 nm. The addition of Si into TiCN resulted in smaller Ra roughness values, indicating a beneficial effect of Si. All investigated surfaces have positive skewness, being adequate for the load bearing implants, which work in a corrosive environment. The hardness of the TiCN coating was 36.6 ± 2.9 GPa and was significantly increased to 47.4 ± 1 GPa when small amounts of Si were added into the TiCN layer structure. A sharp increase in resistance to plastic deformation (H3/E2 ratio) from 0.63 to 1.1 was found after the addition of Si into the TiCN matrix. The most electropositive value of corrosion potential was found for the TiSiCN coating (−14 mV), as well as the smallest value of corrosion current density (49.6 nA cm2), indicating good corrosion resistance in 90% DMEM + 10% FBS, at 37 ± 0.5 °C.

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Improvement of CoCr Alloy Characteristics by Ti-Based Carbonitride Coatings Used in Orthopedic Applications

et al. 2020

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Wettability, Corrosion Resistance, and Osteoblast Response to Reduced Graphene Oxide on CoCr Functionalized with Hyaluronic Acid

et al. 2022

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The durability of metal–metal prostheses depends on achieving a higher degree of lubrication. The beneficial effect of hyaluronic acid (HA) on the friction and wear of both natural and artificial joints has been reported. For this purpose, graphene oxide layers have been electrochemically reduced on CoCr surfaces (CoCrErGO) and subsequently functionalized with HA (CoCrErGOHA). These layers have been evaluated from the point of view of wettability and corrosion resistance in a physiological medium containing HA. The wettability was analyzed by contact angle measurements in phosphate buffer saline-hyaluronic acid (PBS-HA) solution. The corrosion behavior of functionalized CoCr surfaces was studied with electrochemical measurements. Biocompatibility, cytotoxicity, and expression of proteins related to wound healing and repair were studied in osteoblast-like MC3T3-E1 cell cultures. All of the reported results suggest that HA-functionalized CoCr surfaces, through ErGO layers in HA-containing media, exhibit higher hydrophilicity and better corrosion resistance. Related to this increase in wettability was the increase in the expressions of vimentin and ICAM-1, which favored the growth and adhesion of osteoblasts. Therefore, it is a promising material for consideration in trauma applications, with improved properties in terms of wettability for promoting the adhesion and growth of osteoblasts, which is desirable in implanted materials used for bone repair.

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Corrosion Behaviour and J774A.1 Macrophage Response to Hyaluronic Acid Functionalization of Electrochemically Reduced Graphene Oxide on Biomedical Grade CoCr

Chico

Pérez-Maceda

José

et al. 2021

Metals

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Improvements in the lubrication of metal–metal joint prostheses are of great clinical interest in order to minimize the particles released during wear–corrosion processes. In this work, electrochemically reduced graphene oxide (ErGO) on CoCr was functionalized with hyaluronic acid (ErGOHA). Functionalization was carried out by soaking for 24 h in phosphate buffer saline (PBS) solution containing 3 g/L hyaluronic acid (HA). The corrosion performance of CoCrErGO and CoCrErGOHA surfaces was studied by electrochemical impedance spectroscopy (EIS) for 7 days in PBS. Biocompatibility and cytotoxicity were studied in mouse macrophages J774A.1 cell line by the measurement of mitochondrial activity (WST-1 assay) and plasma membrane damage (LDH assay). The inflammatory response was examined through TNF-α and IL-10 cytokines in macrophages culture supernatants, used as indicators of pro-inflammatory and anti-inflammatory responses, respectively. EIS diagrams of CoCrErGOHA revealed two time constants: the first one, attributed to the hydration and diffusion processes of the HA layer adsorbed on ErGO, and the second one, the corrosion resistance of ErGOHA/CoCr interface. Macrophage assays showed better behavior on CoCrErGOHA than CoCr and CoCrErGO surfaces based on their biocompatible, cytotoxic, and inflammatory responses. Comparative analysis of IL-10 showed that functionalization with HA induces higher values of anti-inflammatory cytokine, suggesting an improvement in inflammatory behavior.

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Macrophage Biocompatibility of CoCr Wear Particles Produced under Polarization in Physiological Hyaluronic Acid Solution

Abstract: ABSTRACT

Cited by 4 publications

References 31 publications

Improvement of CoCr Alloy Characteristics by Ti-Based Carbonitride Coatings Used in Orthopedic Applications

Improvement of CoCr Alloy Characteristics by Ti-Based Carbonitride Coatings Used in Orthopedic Applications

Wettability, Corrosion Resistance, and Osteoblast Response to Reduced Graphene Oxide on CoCr Functionalized with Hyaluronic Acid

Corrosion Behaviour and J774A.1 Macrophage Response to Hyaluronic Acid Functionalization of Electrochemically Reduced Graphene Oxide on Biomedical Grade CoCr

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