1978
DOI: 10.1073/pnas.75.10.4992
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Macronuclear DNA of the hypotrichous ciliate Oxytricha fallax

Abstract: DNA in the macronuclei of Oxytricha fallax, as in other hypotrichous ciliate protozoa, exists as small, achromosomal molecules rather than in chromosomes. We report studies on 0. fallax DNA using physicochemical procedures and nucleic acid hybridization. Macronuclear DNA molecules range in size from 22 kilobase pairs (kb) to about 0.5 kb. The DNA has a buoyant density in CsCl of 1.694 gcm-3 and a melting temperature in 15 mM NaCl/1.5 mM sodium citrate, pH 7, at 65.40. These values correspond to 34.7% Gua + Cyt… Show more

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Cited by 73 publications
(32 citation statements)
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“…have both methylcytosine and methyladenine (28,46), while only methylcytosine has been found in the DNA of another green alga, Euglena gracilis (40). In the ciliates Tetrahymena (23), Paramecium (16), Oxytricha (39), and Stylonychia (5) N6MeA is the sole modified base. The function of N6MeA in eucaryotic DNA is unknown.…”
mentioning
confidence: 99%
“…have both methylcytosine and methyladenine (28,46), while only methylcytosine has been found in the DNA of another green alga, Euglena gracilis (40). In the ciliates Tetrahymena (23), Paramecium (16), Oxytricha (39), and Stylonychia (5) N6MeA is the sole modified base. The function of N6MeA in eucaryotic DNA is unknown.…”
mentioning
confidence: 99%
“…A critical point for the detection of m6A in mammalian DNA is the sensitivity of the methodologies used. In this regard, it is noteworthy that current data available, which do not detect m6A in mammalian DNA, have been obtained with techniques Enterobacteriales E.coli 1.4-2.0 (2) Mycobacteria smegmatis 3.0 (90) Mycobacteria tuberculosis 0.45-0.5 (90) Mycoplasma arginini 2.0 (91) Mycoplasma capricolum 0.2 (91) Mycoplasma hyorhinis 2.0 (91) Mycoplasma orale 0.9 (91) Acholeplasma laidlawii 0.5 (91) Eucarya Oxytricha fallax 0.6-0.7 (43) Paramecia aurelia 2.5 (14) Chlamydomonas reinhardi 0.50 (41) Tetrahymena pyriformis 0.65-0.80 (41,48) Tetrahymena thermophila 0.80 (44) Viridiplantae, Wheat 0.09-0.10 (mt) (61) 0.46-0.55 (n) (61) Arthropoda, Aedes albopictus 0.1 (63) mt: mitochondrial, n: nuclear. %m6A were calculated on the basis of total Adenine residues.…”
Section: M6a In Protist Dnamentioning
confidence: 99%
“…The deletion of 6mA methyltransferase in pathogenic Escherichia coli results in global transcription changes, indicating the likelihood of 6mA not only serving as a host genetic marker but also possessing regulatory functions (Fang et al, 2012). Besides bacteria, certain unicellular eukaryotes also have relatively abundant 6mA in their genomes, for instance, the protozoan Tetrahymena (Harrison, Findly, & Karrer, 1986;Hattman, Kenny, Berger, & Pratt, 1978), Oxytricha fallax (Rae & Spear, 1978), Paramecium aurelia (Cummings, Tait, & Goddard, 1974), and green algae Chlamydomonas reinhardtii (Hattman et al, 1978). However, the lack of methylation-sensitive restriction endonucleases suggests that 6mA possesses specific regulatory functions in these unicellular eukaryotes (Ehrlich & Zhang, 1990).…”
Section: Introductionmentioning
confidence: 97%