Macromolecular osteolytic factor synthesised by squamous carcinoma cell lines from the head and neck in vitro is interleukin 1

Meghji, S; Sandy, Jonathan R; Scutt, Andrew; Harvey, W. Proctor; Carter, R. L.; Harris, Malcolm

doi:10.1038/bjc.1988.153

Cited by 9 publications

(1 citation statement)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Invasion of ossified laryngeal cartilages might be due their rich vascularity [2,10,16], the occurrence of a tumor angiogenic factor [7] and/or osteoclast resorption of bone surfaces facing infiltrating tumor [5]. This can be activated by releasing stimulatory substances, such as prostaglandins and interleukin1 [21].…”

Section: Introductionmentioning

confidence: 99%

Expression of c-erb B-2 oncoprotein in cancer of the larynx in relation to invasion of the cartilagenous framework and prognosis

Tantawy

Youins

Hamza

1999

European Archives of Oto-Rhino-Laryngology

View full text Add to dashboard Cite

Oncogenes are important cellular genes that in general promote in the normal growth regulatory pathways. The human c-erb B-2 proto-oncogene (HER-2 or neu) encodes a 185 kDa transmembrane putative growth factor receptor of the tyrosine kinase family. This oncogene has been shown to be over expressed and/or amplified in primary carcinoma of the breast, ovary, pancreas and salivary glands. This study was conducted to evaluate a possible link between amplification of c-erb B-2 oncoprotein and cartilage invasion in laryngeal carcinoma. In addition, data concerning overexpression were compared to other clinicopathological parameters as well as clinical outcomes. In all, 34 patients with squamous cell carcinoma of the larynx were studied prospectively. Total laryngectomy specimens were sliced in horizontal sections at 4- to 5-mm intervals. Specimens were preserved in 10% formalin, and histopathological examinations were carried out after embedding tissues in paraffin sections and then staining them with hematoxylin and eosin. Detection of c-erb B-2 oncoprotein overexpression was carried out with a polyclonal antibody and an avidin-biotin kit. The level of c-erb B-2 overexpression was determined using the Quantimet 520 Leica image analyzing system. However, no significant correlation was found between cartilage invasion and clinicopathological parameters and prognosis. Overexpression of c-erb B-2 attained no significant correlation with clinicopathological parameters. In contrast, the correlation of c-erb B-2 overexpression and cartilage invasion was statistically significant (P = 0.034). In general, overexpression of c-erb B-2 oncoprotein was related to the more aggressive tumors with high capability of invading laryngeal cartilages. Patients with +ve c-erb B-2 oncogene had a poor prognosis but this was not statistically significant when compared to the clinical outcomes of patients with the -ve c-erb B-2 oncogene.

show abstract

Section: Introductionmentioning

confidence: 99%

Expression of c-erb B-2 oncoprotein in cancer of the larynx in relation to invasion of the cartilagenous framework and prognosis

Tantawy

Youins

Hamza

1999

European Archives of Oto-Rhino-Laryngology

View full text Add to dashboard Cite

show abstract

Constitutive production of lymphocyte activating factors by normal tissues in the adult rat

Granholm

Söder

1991

J of Cellular Biochemistry

View full text Add to dashboard Cite

Lymphocyte activating factors (LAFs), e.g., interleukin-1 (IL-1) and IL-1-like factors, have previously been demonstrated outside the immune system in the skin, thymus epithelium, and the human and rat testis. We have studied the presence of LAFs in normal tissues of the adult rat, utilizing a highly IL-1 sensitive murine thymocyte proliferation assay. We have demonstrated high amounts of LAF activity in the tongue, esophagus, proventricular part of the stomach, and the liver. Some activity was also demonstrated in the duodenum, placenta, spleen, Peyer's patches, glandular stomach, and jejunum, but no bioactivity was present in other gastrointestinal, endocrine, lymphoid, or haematopoeitic tissues. We were also unable to detect any LAF activity in the reproductive organs (except for the testis), urinary tract, skeletal and muscular tissues, brain, eyes, salivary glands, or lung. In the esophagus the activity was mainly localized to the mucosa. The LAF activity in the skin was partly inhibited by treatment with a mixture of antibodies against human IL-1 alpha and IL-1 beta. Dose response curves and gel filtration on a Sephacryl S-200 column suggested the presence of a high molecular weight (90,000-100,000 Da) LAF inhibitory factor in the liver. In all positive tissues, the demonstrated LAFs had a molecular weight of 15,000-25,000 Da, as determined by Sephacryl S-200 gel filtration. Of the positive tissues, the skin, tongue, esophagus, and the proventricular part of the stomach all contain stratified squamous epithelium. It is tempting to suggest that the detected LAFs have a similar function in these barrier tissues, e.g., to serve as host defence factors, or, alternatively or additionally, as tissue growth factors.

show abstract

Cell, tissue and organ culture as in vitro models to study the biology of squamous cell carcinomas of the head and neck

Sacks

1996

Cancer Metast Rev

105

View full text Add to dashboard Cite

In vitro models are currently being used to study head and neck squamous cell carcinoma (HNSCC). Several hundred HNSCC cell lines have been established by various investigators and used to study a broad spectrum of questions related to head and neck cancer. The head and neck model with respect to multistage carcinogenesis is now complete. Several techniques exist for the culture of normal epithelial cells from the upper aerodigestive tract (UADT). The biology of these UADT cells (oral cavity, oropharynx, hypopharynx and larynx) is being studied. Successful culture of premalignant lesions (dysplastic mucosa, leukoplakia, erythroplakia) has resulted in establishment of a limited number of premalignant cell lines and cell cultures. HPV infection of normal oral epithelial cells for immortalization (approximately premalignant cells) coupled with transformation with carcinogens (malignant cells) has established an experimental model for progression. Two in vivo models for oral carcinogenesis, the 7,12 dimethylbenz(a)anthracene-induced hamster cheek pouch model and the 4-nitroquinoline-N-oxide rat oral model, have been established in culture. Thus, multistage carcinogenesis models have been established from both human tissues and animal models and include cultures of normal, premalignant and malignant cells. Culture techniques for growing dissociated primary tumor cells for short term experimental analysis are being used. The culture of normal or tumor tissue as organ/explant cultures allows for the maintenance of normal cell-cell and cell-matrix interaction, but limits experimentation since these cultures cannot be propagated. Several three dimensional model systems are being used to obtain this histological complexity but allow for experimentation. The ability to culture normal, premalignant and malignant cells coupled with the use of a variety of culture techniques, should allow for the continued growth and experimentation in head and neck cancer research.

show abstract

Macromolecular osteolytic factor synthesised by squamous carcinoma cell lines from the head and neck in vitro is interleukin 1

Cited by 9 publications

References 35 publications

Expression of c-erb B-2 oncoprotein in cancer of the larynx in relation to invasion of the cartilagenous framework and prognosis

Expression of c-erb B-2 oncoprotein in cancer of the larynx in relation to invasion of the cartilagenous framework and prognosis

Constitutive production of lymphocyte activating factors by normal tissues in the adult rat

Cell, tissue and organ culture as in vitro models to study the biology of squamous cell carcinomas of the head and neck

Contact Info

Product

Resources

About