Macromolecular Complexation between Bovine Serum Albumin and the Self-Assembled Hydrogel Nanoparticle of Hydrophobized Polysaccharides

Abstract

Macromolecular complexation between bovine serum albumin (BSA) and self-assembled hydrogel nanoparticle formed by the self-aggregation of cholesterol-bearing pullulan (CHP) was studied by high performance size exclusion column chromatography (HPSEC) and circular dichroism (CD). The CHP self-aggregates complexed with one BSA molecule to give colloidally stable nanoparticles (R G = 17 nm) at pH 7.0 and 25 °C. This was almost irrespective of the substitution degree of the cholesterol group of CHP. The helical content of BSA decreased upon complexation. Unfolding of BSA by either heating or a denaturant such as urea was largely suppressed upon complexation. BSA would be incorporated inside into the hydrogel matrix of the CHP nanoparticle. Kinetic analysis of the complexation suggested a two-step process: namely, the fast pre-equilibrium of looser binding of BSA to the CHP self-aggregate followed by the slower process of tighter inclusion into the hydrogel network. The substitution degree of the cholesterol group in CHP significantly affected the complexation kinetics.

Controlled release and interaction of protein using self‐assembling peptide RATEA16 nanofiber hydrogels

“…Especially, the hydrophobic interaction between the hydrophobic domain and the hydrophobic surface of insulin exposed by part denaturation is very important, and is the main driving force for the denaturation of the insulin-FITC native structure. 19,24,[29][30][31][32] Possibly due to part denaturation, it was observed that the hydrophilic insulin-FITC molecules immediately became precipitate, when adding peptide RATEA16 into its aqueous solution (see Supporting Information). At the same time, the extent to b-sheet secondary structure of peptide increased, indicating that the existence of insulin-FITC molecules redounded to the b-sheet formation of peptide RATEA16.…”

Fast responsive and morphologically robust thermo-responsive hydrogel nanofibres from poly(N-isopropylacrylamide) and POSS crosslinker

“…Indeed, thermoresponsive hydrogel nanoparticles and mesoporous membranes have been reported. 8 However, it is difficult to construct mechanically robust macrostructures by using nanoparticles alone. Mesoporous membranes, on the other hand, show slow response due to the small pore size, but good retention of morphology.…”

Nanoparticles Based on Modified Polysaccharides

“…Various cholesterol-bearing pullulans with different MWs of the parent pullulan and DS of the cholesteryl moiety was synthesized (Nishikawa et al, 1996;Akiyoshi et al, 1997). Irrespective of the MW of the parent pullulan and the DS, all of cholesterol-pullulans provided unimodal and mono-disperse self-aggregates in water.…”