Antimicrobial poly(vinyl alcohol) nanofibres are produced using AquaQure biocide as additive to the polymer solution. AquaQure is an aqueous antimicrobial agent containing mainly Cu2+ and Zn2+. Antimicrobial tests show that the fibres achieve up to a 5 log reductions in populations of E. coli, S. aureus, S. typhimurium, P. aeruginosa and K. pneumoniae bacteria. Reusability of the nanofibre membranes is investigated to establish if the nanofibres retain their morphology and antimicrobial effectiveness over six cycles of water filtration. Leaching of AquaQure constituents from the nanofibres into filtered water is assessed and found to be at acceptable levels.