Macromol. Biosci. 7/2011

Rungta, Parul; Bandera, Yuriy; Roeder, Ryan D.; Li, Yangchun; Baldwin, William S.; Sharma, Deepti; Sehorn, Michael G.; Luzinov, Igor; Foulger, Stephen H.

doi:10.1002/mabi.201190018

Cited by 4 publications

(14 citation statements)

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“…37 The fluorophore immobilization on nanoparticle surfaces can yield significant photophysical benefits and is being increasingly investigated for roles in nanomedicine, nanobiotechnology and fluorescence imaging. [38][39][40] The surface modification can also modulate inflammatory cell recruitment of carbon nanoparticles. 41 In the present system a near-infrared boron azadipyrromethene (NIR-AZA) dye 1 was chosen as the fluorophore label (Scheme 1).…”

Section: Introductionmentioning

confidence: 99%

Multimodal Microscopy Distinguishes Extracellular Aggregation and Cellular Uptake of Single‐Walled Carbon Nanohorns

Devereux

Cheung²,

Daly³

et al. 2018

Chemistry A European J

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The low toxicity, high surface area, and ease of functionalisation of carbon nanohorns (CNH) makes them attractive systems for cellular imaging, diagnostics and therapeutics. However, challenges remain for the biomedical translation of these and other nanomaterials. A significant task is tuning the surface chemistry to achieve optimal cellular interactions. Herein, we combine real-time fluorescent imaging of nanoparticle cellular uptake and real-time differential interference contrast (DIC) imaging of extracellular media to monitor a) nanoparticle/nanoparticle and b) nanoparticle/cell interactions for CNHs covalently modified with an OFF/ON near-IR dye, the fluorescence of which is switched OFF in extracellular environments and triggered upon cellular internalisation. CHN samples modified with different loadings of the hydrophobic dye are taken as a simple model of drug-loaded nanoparticle systems. The punctate fluorescence suggests the CNHs are delivered to lysosomes and other vesicles of the endocytic pathway. DIC imaging highlights the competition that exists for many particle types, between extracellular aggregation and cellular internalization, the efficiency of which would be dependent upon the amount of fluorophore loading. The results of this study illustrate how complementary real-time imaging methods together with physicochemical characterisation can be used to address the challenges involved in optimising nanoparticle/cell interactions for biomedical applications.

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Section: Introductionmentioning

confidence: 99%

Multimodal Microscopy Distinguishes Extracellular Aggregation and Cellular Uptake of Single‐Walled Carbon Nanohorns

Devereux

Cheung²,

Daly³

et al. 2018

Chemistry A European J

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“…1 H and 13 C NMR spectra were recorded on JEOL ECX-300 spectrometers (300 MHz for proton and 76 MHz for carbon). Chemical shis for protons are reported in parts per million downeld from tetramethylsilane and are referenced to residual protium in the NMR solvent (CDCl 3 : d 7.26 ppm, DMSO-d6: d 2.50 ppm).…”

Section: Chemical Characterization Methodsmentioning

confidence: 99%

“…The azPEG was prepared according to the method published. 13 Mono-methoxy-PEG5000-methansulfonate. Methylsulfonyl chloride (0.92 g, 8 mmol) in dichloromethane (5 mL) was added dropwise at room temperature to the stirring solution of triethylamine (0.89 g, 8.8 mmol) and mono-methoxy-PEG5000 (20 g, 4 mmol) in dichloromethane (70 mL).…”

Section: Preparation Of Azide-modied Polyethylene Glycol (Azpeg)mentioning

confidence: 99%

“…6,8 The underlying mechanism for activation is a conversion of an aggregated quenched uorophore back to its emissive monomeric state. To that end, the signicant variations in the emission spectra of photoluminescent dyes which are routinely observed when they form supramolecular host/guest assemblies or complex with biomacromolecules [9][10][11][12] and have been exploited in designing activatable contrast agents by this group 13 as well as others. 14 As indicated earlier, in addition to activating the emission only in the tissue of interest, to achieve maximum TBR, the contrast agent must accumulate in the targeted tissue while clearing rapidly from non-target organs and tissues.…”

Section: Introductionmentioning

confidence: 99%

“…[27][28][29][30][31] Within the nanodevice conceptualization, the design of a sub-100 nm particle that exhibits NIR uorescence that can be modulated on or off by specic proteins within a cancer cell is of particular interest. 13,14 In the current effort, poly(propargyl acrylate) (PA) colloids were surface modied with an azide terminated squarainebased dye, a NIR emitting uorophore, and polyethylene glycol through a copper-catalyzed azide/alkyne cycloaddition performed in water. 32 The placement of the uorophore onto the surface of the particles allows for the dye to form a guest/host complex with available proteins and this feature was exploited in achieving a uorescence activation of the particles when in a quenching aqueous medium.…”

Section: Introductionmentioning

confidence: 99%

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Protein triggered fluorescence switching of near-infrared emitting nanoparticles for contrast-enhanced imaging

et al. 2013

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Sub-100 nm colloidal particles which are surface-functionalized with multiple environmentally-sensitive moieties have the potential to combine imaging, early detection, and the treatment of cancer with a single type of long-circulating "nanodevice". Deep tissue imaging is achievable through the development of particles which are surface-modified with fluorophores that operate in the nearinfrared (NIR) spectrum and where the fluorophore's signal can be maximized by "turning-on" the fluorescence only in the targeted tissue. We present a general approach for the synthesis of NIR emitting nanoparticles that exhibit a protein triggered activation/deactivation of the emission.Dispersing the particles into an aqueous solution, such as phosphate buffered saline (PBS), resulted in an aggregation of the hydrophobic fluorophores and a cessation of emission. The emission can be reinstated, or activated, by the conversion of the surface-attached fluorophores from an aggregate to a monomeric species with the addition of an albumin. This activated probe can be deactivated and returned to a quenched state by a simple tryptic digestion of the albumin. The methodology for emission switching offers a path to maximize the signal from the typically weak quantum yield inherent in NIR fluorophores.

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Organic Near‐Infrared‐Emitting Nanoparticles for X‐Ray Bioimaging: A Nontoxic Alternative Harnessing Förster Resonance Energy Transfer‐Based Sequential Energy Transfer

Jones,

Bandera,

Foulger

et al. 2024

Advanced Photonics Research

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In the efforts to generate a less toxic X‐Ray bioimaging contrast agent, a fully organic, radioluminescent nanoparticle system that emits in the near‐infrared (NIR) region when excited with an X‐Ray source is synthesized using a two‐step process. First, red‐emitting nanoparticles are fabricated by the emulsion copolymerization of styrene and propargyl acrylate with anthracene, naphthalimide, and rhodamine B methyl methacrylate derivatives. Subsequently, the nanoparticles are modified with silicon phthalocyanine and indocyanine green derivatives via a copper(I)‐catalyzed azide/alkyne cycloaddition “click” reaction. By coupling an organic scintillator with four Förster resonance energy transfer‐pairing dyes, X‐Ray‐induced, multiple, sequential energy transfer is exploited to convert ionizing radiation from an X‐Ray source into NIR light, which is optimal for biomedical imaging. Proof‐of‐concept imaging studies show that the X‐Ray‐induced indocyanine green fluorescence from the particulate system can be visualized through porcine tissue. Additionally, toxicity studies in human embryonic kidney cells indicate that the particles are nontoxic and applicable in vivo.

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Macromol. Biosci. 7/2011

Cited by 4 publications

References 0 publications

Multimodal Microscopy Distinguishes Extracellular Aggregation and Cellular Uptake of Single‐Walled Carbon Nanohorns

Multimodal Microscopy Distinguishes Extracellular Aggregation and Cellular Uptake of Single‐Walled Carbon Nanohorns

Protein triggered fluorescence switching of near-infrared emitting nanoparticles for contrast-enhanced imaging

Organic Near‐Infrared‐Emitting Nanoparticles for X‐Ray Bioimaging: A Nontoxic Alternative Harnessing Förster Resonance Energy Transfer‐Based Sequential Energy Transfer

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