Macromol. Biosci. 5/2009

Mi, Kun; Wang, Guixia; Liu, Zijia; Feng, Zhi-Hua; Huang, Benjamin; Zhao, Xiaobo

doi:10.1002/mabi.200990007

Cited by 6 publications

(7 citation statements)

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“…From the horizontal view (Figure 5a,b, middle), it was apparent that the majority of cells were located within a 60-100 µm layer. This is a significant depth, as mast cells size are ≈8-10 µm in diameter [23] (also shown in Figure 5e,f), meaning that adherent cells are likely found within the matrix, as opposed to that in a 2D plane on the matrix surface. The 2D images show that IgEsensitized BMMCs with a matrix generally keep the similar round-shape-like unsensitized BMMCs (Figure 5e), but the IgE sensitization leads to enhancement of the cortical F-actin ring (Figure 5f).…”

Section: The Morphology Of Adherent Mast Cell In Nanoscaffold Matrixmentioning

confidence: 90%

“…[17][18][19][20][21][22] Transmission electron microscopy (TEM) and microrheological analysis showed that 0.5% wt (RADA) 4 formed a more-rigid hydrogel compared to the same concentration of collagen I and Matrigel due to the self-assembling longer nanofiber and more crosslinks. [17,23] These types of self-assembling peptides have been employed in various in vitro and in vivo studies, including 3D cell culture, [16] wound healing, [24] and tissue repair. [25] Due to the fact that these self-assembling peptides can be programmed at the molecular level with biochemical information (i.e., bioactive peptides and lipids), they may be particularly suited for developing an ECM mimic for fundamental cell-biology studies.…”

Section: Introductionmentioning

confidence: 99%

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The Spontaneous Adhesion of BMMC onto Self‐Assembled Peptide Nanoscaffold without Activation Inhibits Its IgE‐Mediated Degranulation

Lü

Arizmendi

Kulka

et al. 2017

Adv Healthcare Materials

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Mast cells play a distinct role in the innate immune response. Engineered microenvironments for the express purpose of influencing mast cell activity will provide a novel means of designing biomaterials, as well as a means to systematically investigate mast cell biology in a 3D setting. Here, the effect of nanoscaffolds composed of self-assembling peptides, namely (RADA) , on bone-marrow-derived murine mast cell (BMMC) activity is reported. Unlike most studies that stimulate mast cells to induce adhesion, this results show that BMMCs spontaneously adhere to the artificial nanoscaffold without initiating their activation. It is observed that the classical immunoglobulin (IgE) antigen-mediated degranulation of adhered BMMC is inhibited by the nanoscaffold, while non-IgE (A23187)-induced degranulation is unaffected. The inhibition of IgE-antigen-mediated degranulation is likely a result of inhibited molecular diffusion within the matrix; antigen diffusion, IgE-FcεRI complex shuttling, and/or formation of multiple IgE-FcεRI clusters may be physically hindered in the presence of the polyvalent nanofiber network. Moreover, the IgE/antigen-induced inflammatory cytokine tumor necrosis factor α release from adherent BMMCs is significantly reduced likely due to interaction with the nanofiber matrix. This work is considered the first step in quantifying mast cell activity in artificial matrices composed of self-assembling peptides.

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Section: The Morphology Of Adherent Mast Cell In Nanoscaffold Matrixmentioning

confidence: 90%

Section: Introductionmentioning

confidence: 99%

The Spontaneous Adhesion of BMMC onto Self‐Assembled Peptide Nanoscaffold without Activation Inhibits Its IgE‐Mediated Degranulation

Lü

Arizmendi

Kulka

et al. 2017

Adv Healthcare Materials

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“…In fact, the application of cell-culturing hydrogels using peptide materials has been already in practical use. 19,20 Many works have reported the safety and affinity between the peptide hydrogel and cell, [21][22][23] additionally, the in vivo experiment using a mouse, in which the hydrogel for the drug delivery vehicle is injected into mouse's cavum abdominis, indicated the potential of the peptide hydrogel in practical applications. 24 Therefore, the peptide hydrogel is one of the powerful candidate for novel bone-filling materials from the viewpoint of above specific properties.…”

Section: Introductionmentioning

confidence: 99%

Calcium phosphate biomineralization in peptide hydrogels for injectable bone-filling materials

et al. 2012

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We performed amorphous calcium phosphate (ACP) and hydroxyapatite (HAp) mineralization in peptide hydrogels for the formation of a novel bone-filling material. We prepared two kinds of b-sheet peptides, (LE) 8 and (VEVSVKVS) 2 , respectively, using hydrophilic glutamic acid (E), serine (S) and lysine (K), and hydrophobic leucine (L) and valine (V). Both peptides hierarchically self-assembled as nanofibers and formed hydrogels in the presence of calcium ions. The formation of the hydrogel was due to the ionic cross-linkage between carboxyl groups in the glutamic acid side chains of the peptide nanofibers and the calcium ion. (LE) 8 formed a clear hydrogel above a calcium ion concentration of 4.0 Â 10 À3 M and the hydrogel collapsed at 1.0 Â 10 À2 M, owing to excess ionic cross-linkage. On the other hand, (VEVSVKVS) 2 containing two glutamic acid residues per molecule retained the hydrogel structure at a higher concentration of calcium ions in the hydrogel where the (LE) 8 hydrogel collapsed. The viscoelastic property of both peptide hydrogels was increased by increasing the calcium ion concentration, showing adequate strength as a bone-filling material. When phosphate ion was added into the (LE) 8 hydrogel containing calcium ion, ACP was mineralized along the peptide nanofiber in the hydrogel under neutral pH. The (VEVSVKVS) 2 hydrogel, on the other hand, produced HAp under basic pH. In addition, the peptide hydrogel smoothly recovered its original moduli just after the shear deformation of the hydrogel. It was also clarified that calcium ions are not only a source of mineralization but also induce an increase in the mechanical strength of the hydrogels as a reinforcing agent. It was shown, moreover, that the crystallinity of the HAp was slightly dependent on the modulus of the peptide hydrogels. Therefore, it may be said that peptide hydrogels are applicable for tailor-made injectable bone-filling materials.

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“…Self‐assembling d‐EAK16 nanofibers also showed a rapid hemostasis of approximately 20 s in a rabbit liver wound healing model 194. Furthermore, self‐assembling peptides effectively reduced the malignant phenotype of the tumor cells in vivo in comparison with collagen type I and Matrigel 195. An optimal paclitaxel pharmacokinetics was obtained when this drug was incorporated into PLGA nanofibers to treat malignant glioblastoma in mice 196.…”

Section: Medicinementioning

confidence: 99%

Biological, Chemical, and Electronic Applications of Nanofibers

Nguyen

Chen

Elumalai

et al. 2012

Macro Materials & Eng

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With their high‐surface‐to‐volume ratio, nanofibers have been postulated to increase interactions between nanofibrous materials and targeted substrates, which are helpful to overcome many obstacles and enhance the efficiency in a diverse number of applications. Over the past decade, many studies have been published on the fabrication of nanofibers and their applications in various fields. In this review, novel biological, chemical, and electrical characteristics of nanofibers as well as their recent status and achievements in medicine, chemistry, and electronics are analyzed. It is found that nanofibers can induce fast regeneration of many tissues/organs in medical applications and improve the efficiency of many chemical and electronics applications.

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Macromol. Biosci. 5/2009

Cited by 6 publications

References 0 publications

The Spontaneous Adhesion of BMMC onto Self‐Assembled Peptide Nanoscaffold without Activation Inhibits Its IgE‐Mediated Degranulation

The Spontaneous Adhesion of BMMC onto Self‐Assembled Peptide Nanoscaffold without Activation Inhibits Its IgE‐Mediated Degranulation

Calcium phosphate biomineralization in peptide hydrogels for injectable bone-filling materials

Biological, Chemical, and Electronic Applications of Nanofibers

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