Macro‐ et microdosage de traces de cobalt. VI. Dosage du cobalt dans le sang

Abstract

A quantitative method for the analysis of cobalt in 50 to 100 g of normal whole blood, without anticoagulants, by spectrophotometry using nitroso‐R salt, is discussed. By the use of radiocobalt 60 as a tracer, the loss can be estimated. With a final volume of 0.8 ml of solution, the spectrophotometric measurement is carried out on 0.4 ml contained in a microcell placed on a suitable support, as already described in a previous paper. The quantity of cobalt contained in a sample of normal blood is found to be 0.33 ppb with a relative error of ± 10%. The time necessary for one such assay is about 22 hours, of which 12 are without any special control; that for a series of 10 analyses is not sensibly different.

The concentrations of cobalt, copper, iron and zinc in some normal human tissues as determined by neutron-activation analysis

“…Of the several methods that have been described for the determination of cobalt in biological materials by activation analysis (Koch, Smith, Shimp & Connor, 1956;Haerdi, Vogel, Monnier & Wenger, 1960;Kaiser & Meinke, 1960;Pijck, Gills & Hoste, 1961), none possesses the sensitivity and specificity necessary for its estimation at the concentrations existing naturally in most tissues, particularly when only small samples are available for analysis.…”