Macro and microscopic analysis of Alfredia nivea KAR. & KIR herb

Abstract

Kazakhstan flora is rich in promising little-studied plants that are used in folk medicine, but additional deep research is required for their introduction into medical practice with the help of modern scientific techniques. An interesting object for introduction into the official medical and pharmaceutical practice is the Alfredia Nivea Kar. & Kir, family Asteraceae, whose extracts have a neurotropic effect.
 The aim. Regarding mentioned above, in order to create new medicines based on the grass of the Alfredia nivea, it is necessary to develop methods of its macro and microscopic identification.
 Materials and methods. Macro- and microscopic studies of the grass of Alfredia nivea were carried out in accordance with the methodology of SPhU 2.8.23 "Microscopic examination of medicinal vegetable raw materials". Macroscopic studies were performed using magnifying glass and binocular microscope MBS-9, microscopic-using MS Microscopes 10 (eyepiece X5, X10, X15, lenses x10, x40), micromed XS-4130 (WF15X, Opera, lens X40/0, 65, x10/0,25) with microphoto addon (China).
 Results. The morphological and anatomical features of the Alfredia nivea grass. Diagnostic features of the structure of stems, leaves and flowers of this species were revealed.
 Conclusions. The macroscopic and anatomical identification parameters of the grass of the Alfredia of Snow. The study of the morphological and anatomical features of the stems, leaves and flowers of Alfredia nivea, considering the requirements of European pharmacopoeia. Diagnostic features of the structure of stems, leaves and flowers of the species were found, which were used as key quality indicators for standardization and identification of raw materials

Standardization Parameters of Alfredia nivea KAR.&KIR Herb

“…Based on the results of the microscopic analysis of the A. nivea herb [14], such microscopic signs, which served as the basis for the development of Identification B, were determined.…”

“…Previously, the standardization of the A. cernua herb [13], the macroscopic and microscopic study of the A. nivea herb were carried out, and its diagnostic signs were determined [14]. These data were taken into account when developing a project of quality control methods for the A. nivea herb.…”

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“…Морфологічний аналіз шавлії блискучої проводили на свіжих і висушених зразках із використанням лупи (х10). Мікроскопічний аналіз трави проводили відповідно до сучасних загальноприйнятих методик (Serbin, 2006;Rustemkulov, 2023). Сировину попередньо замочували в суміші «етанол -гліцерин -вода» (у співвідношенні 1 : 1 : 1).…”