Macro‐alignment of electrospun fibers for vascular tissue engineering

Zhu, Yabin; Cao, Yilin; Pan, Jin; Liu, Yuxin

doi:10.1002/jbm.b.31544

Cited by 87 publications

(67 citation statements)

References 33 publications

(35 reference statements)

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“…2 In addition, electrospinning produces nanoarchitecturely patterned fibers in random or aligned form and it has been demonstrated to greatly influence the cell orientation and function, especially in nerve and muscle TE. [3][4][5][6][7] Electrospun aligned nanofibers could also provide better orientation of cardiac cells and might serve as potential scaffolds for cardiac reconstruction. Chemical composition of a scaffold might also be crucial cues, as it affects protein adhesion and distribution, and further influences the cell orientation and positioning.…”

Section: Introductionmentioning

confidence: 99%

Guided orientation of cardiomyocytes on electrospun aligned nanofibers for cardiac tissue engineering

et al. 2011

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Cardiac tissue engineering (TE) is one of the most promising strategies to reconstruct the infarct myocardium and the major challenge involves producing a bioactive scaffold with anisotropic properties that assist in cell guidance to mimic the heart tissue. In this study, random and aligned poly(ε-caprolactone)/gelatin (PG) composite nanofibrous scaffolds were electrospun to structurally mimic the oriented extracellular matrix (ECM). Morphological, chemical and mechanical properties of the electrospun PG nanofibers were evaluated by scanning electron microscopy (SEM), water contact angle, attenuated total reflectance Fourier transform infrared spectroscopy and tensile measurements. Results indicated that PG nanofibrous scaffolds possessed smaller fiber diameters (239 ± 37 nm for random fibers and 269 ± 33 nm for aligned fibers), increased hydrophilicity, and lower stiffness compared to electrospun PCL nanofibers. The aligned PG nanofibers showed anisotropic wetting characteristics and mechanical properties, which closely match the requirements of native cardiac anisotropy. Rabbit cardiomyocytes were cultured on electrospun random and aligned nanofibers to assess the biocompatibility of scaffolds, together with its potential for cell guidance. The SEM and immunocytochemical analysis showed that the aligned PG scaffold greatly promoted cell attachment and alignment because of the biological components and ordered topography of the scaffolds. Moreover, we concluded that the aligned PG nanofibrous scaffolds could be more promising substrates suitable for the regeneration of infarct myocardium and other cardiac defects.

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Section: Introductionmentioning

confidence: 99%

Guided orientation of cardiomyocytes on electrospun aligned nanofibers for cardiac tissue engineering

et al. 2011

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“…Groups interested in vascular graft engineering have found that aligned electrospun fibres can influence vascular cell phenotypes as well. Zhu et al report that fibrin-coated aligned PCL fibres enhanced vascular endothelial cell adhesion and von Willebrand factor production over cells grown on polystyrene plates (figure 3a,b; Zhu et al 2010). In a similar illustration of the effects of aligned electrospun fibres, Huijun and colleagues demonstrated that superaligned poly-L-lactic acid fibres could promote peripheral blood endothelial cell proliferation and specify cell orientation.…”

Section: Electrospinningmentioning

confidence: 95%

Engineering extracellular matrix through nanotechnology

Kelleher

Vacanti

2010

J. R. Soc. Interface.

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The goal of tissue engineering is the creation of a living device that can restore, maintain or improve tissue function. Behind this goal is a new idea that has emerged from twentieth century medicine, science and engineering. It is preceded by centuries of human repair and replacement with non-living materials adapted to restore function and cosmetic appearance to patients whose tissues have been destroyed by disease, trauma or congenital abnormality. The nineteenth century advanced replacement and repair strategies based on moving living structures from a site of normal tissue into a site of defects created by the same processes. Donor skin into burn wounds, tendon transfers, intestinal replacements into the urinary tract, toes to replace fingers are all examples. The most radical application is that of vital organ transplantation in which a vital part such as heart, lung or liver is removed from one donor, preserved for transfer and implanted into a patient dying of end-stage organ failure. Tissue engineering and regenerative medicine have advanced a general strategy combining the cellular elements of living tissue with sophisticated biomaterials to produce living structures of sufficient size and function to improve patients' lives. Multiple strategies have evolved and the application of nanotechnology can only improve the field. In our era, by necessity, any medical advance must be successfully commercialized to allow widespread application to help the greatest number of patients. It follows that business models and regulatory agencies must adapt and change to enable these new technologies to emerge. This brief review will discuss the science of nanotechnology and how it has been applied to this evolving field. We will then briefly summarize the history of commercialization of tissue engineering and suggest that nanotechnology may be of use in breeching the barriers to commercialization although its primary mission is to improve the technology by solving some remaining and vexing problems in its science and engineering aspects.

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“…The scaffolds are porous and have fibers that can be tailored to affect cell differentiation, proliferation, and migration [34][35][36][37][38]. These characteristics make electrospun constructs ideal for wound dressings [39,40] and grafts for various tissues such as skin [32,[41][42][43][44][45][46], nerves [32,33,[47][48][49][50][51], vasculature [32,33,41,[51][52][53][54][55][56][57][58][59][60][61][62][63][64][65][66], muscle [33,51,67,68], bone [15,32,33,41,51,[69]…”

Section: Electrospinningmentioning

confidence: 99%

A review of electrospinning manipulation techniques to direct fiber deposition and maximize pore size

et al. 2017

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Electrospinning has been widely accepted for several decades by the tissue engineering and regenerative medicine community as a technique for nanofiber production. Owing to the inherent flexibility of the electrospinning process, a number of techniques can be easily implemented to control fiber deposition (i.e. electric/magnetic field manipulation, use of alternating current, or air-based fiber focusing) and/or porosity (i.e. air impedance, sacrificial porogen/sacrificial fiber incorporation, cryo-electrospinning, or alternative techniques). The purpose of this review is to highlight some of the recent work using these techniques to create electrospun scaffolds appropriate for mimicking the structure of the native extracellular matrix, and to enhance the applicability of advanced electrospinning techniques in the field of tissue engineering.

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Macro‐alignment of electrospun fibers for vascular tissue engineering

Cited by 87 publications

References 33 publications

Guided orientation of cardiomyocytes on electrospun aligned nanofibers for cardiac tissue engineering

Guided orientation of cardiomyocytes on electrospun aligned nanofibers for cardiac tissue engineering

Engineering extracellular matrix through nanotechnology

A review of electrospinning manipulation techniques to direct fiber deposition and maximize pore size

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