Mach–Zehnder interferometer (MZI) point-of-care system for rapid multiplexed detection of microRNAs in human urine specimens

Liu, Qing; Shin, Yong Moon; Kee, Jack Sheng; Kim, Kyoung Woo; Rafei, Siti Rafeah Mohamed; Perera, Agampodi Promoda; Tu, Xiaoguang; Lo, G. Q.; Ricci, E.; Colombel, Marc; Chiong, Edmund; Thiery, Jean Paul; Park, Mi Kyoung

doi:10.1016/j.bios.2015.04.052

Cited by 62 publications

(42 citation statements)

References 47 publications

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“…Less frequent, upregulation of certain let-7 family members has also been observed, suggesting that let-7 does not play a tumour suppressor function under all circumstances and in all tissues ( 6 ). Higher levels of let-7 miRNAs in urine has been reported in bladder cancer ( 7 ). There are no data about urinary let-7 miRNAs in RCC, but increased urinary levels of some of the let-7 family members were found in patients with autosomal dominant polycystic kidney disease suggesting their abundance in urine and therefore also potential utility in other renal diseases such as cancer ( 8 ).…”

Section: Introductionmentioning

confidence: 90%

Detection of let-7 miRNAs in urine supernatant as potential diagnostic approach in non-metastatic clear-cell renal cell carcinoma

et al. 2017

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IntroductionUrinary microRNAs (miRNAs) are emerging as a clinically useful tool for early and non-invasive detection of various types of cancer. The aim of this study was to evaluate whether let-7 family miRNAs differ in their urinary concentrations between renal cell carcinoma (RCC) cases and healthy controls.Materials and methodsIn the case-control study, 69 non-metastatic clear-cell RCC patients and 36 gender/age-matched healthy controls were prospectively enrolled. Total RNA was purified from cell-free supernatant of the 105 first morning urine specimens. Let-7 family miRNAs were determined in cell-free supernatant using quantitative miRNA real-time reverse-transcription PCR and absolute quantification approach.ResultsConcentrations of all let-7 miRNAs (let-7a, let-7b, let-7c, let-7d, let-7e and let-7g) were significantly higher in urine samples obtained from RCC patients compared to healthy controls (P < 0.001; P < 0.001; P = 0.005; P = 0.006; P = 0.015 and P = 0.002, respectively). Subsequent ROC analysis has shown that let-7a concentration possesses good ability to differentiate between cases and controls with area under curve being 0.8307 (sensitivity 71%, specificity 81%).ConclusionsWe have shown that let-7 miRNAs are abundant in the urine samples of patients with clear-cell RCC, and out of six let-7 family members, let-7a outperforms the others and presents promising non-invasive biomarker for the detection of RCC.

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Section: Introductionmentioning

confidence: 90%

Detection of let-7 miRNAs in urine supernatant as potential diagnostic approach in non-metastatic clear-cell renal cell carcinoma

et al. 2017

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“…In addition to established label free detection modalities, such as conventional Kretschmann geometry surface plasmon resonance 84 , other sensing mechanisms have been explored for applications in miRNA analysis, including nano-particle scattering on flexible silicon substrates, 85 surface enhanced Raman spectroscopy, 86 functionalized gold nanoprisims attached to silanized glass, 87 Mach-Zehender interferometers, 88 and total internal fluorescence microscopy. 89 An impressive gold nanowire plasmonic based detection mechanism was developed as a microfluidic lateral flow assay.…”

Section: Biosensor Methodsmentioning

confidence: 99%

Emerging Biosensing Approaches for microRNA Analysis

2015

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“…Hofmann et al [84] proposed a double-stranded ligation assay that yields results in 30 min. Liu et al [85] presented a Mach-Zehnder interferometer-based approach with an even shorter turnaround time of 15 min. Labib and Berezovski [86] reviewed electrochemical-based detection strategies.…”

Section: New Measurement Devicesmentioning

confidence: 98%

Specific miRNA Disease Biomarkers in Blood, Serum and Plasma: Challenges and Prospects

2016

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Significant effort has been devoted to discovering microRNA (miRNA) disease biomarkers. In particular, miRNAs in whole blood or specific blood components are candidates for improving the diagnosis of diseases, including life-threatening pathologies. This review covers the challenges crucial for the translation of miRNAs in body fluids (circulating miRNAs) from a research setting into a clinical care scenario. First, we discuss the specificity of miRNA biomarkers for the diagnosis of a disease. While single miRNAs such as miR-20a, miR-21, miR-155, and miR-126 are frequently not disease specific, miRNA signatures that consist of a plurality of different miRNAs may help to improve differentiation between pathologies. Second, we discuss the degree of reproducibility and highlight selected validation studies. While single miRNA markers are often confirmed by independent studies, miRNA signatures are less frequently verified. Third, we address challenges to the profiling of miRNAs in high-throughput settings and we discuss the appropriateness of various analytical platforms and bioinformatics towards a clinical application of miRNAs. Finally, we shed light on the suitability of enriched miRNA sources, e.g. fractionation of body fluids for extracellular vesicles such as exosomes or blood cells, to develop miRNA signatures. With an increasing number of verified miRNA signatures and with the advance of matured medium-throughput approaches in clinical settings, specific miRNA markers are increasingly likely to contribute to human healthcare.

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Mach–Zehnder interferometer (MZI) point-of-care system for rapid multiplexed detection of microRNAs in human urine specimens

Cited by 62 publications

References 47 publications

Detection of let-7 miRNAs in urine supernatant as potential diagnostic approach in non-metastatic clear-cell renal cell carcinoma

Detection of let-7 miRNAs in urine supernatant as potential diagnostic approach in non-metastatic clear-cell renal cell carcinoma

Emerging Biosensing Approaches for microRNA Analysis

Specific miRNA Disease Biomarkers in Blood, Serum and Plasma: Challenges and Prospects

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