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Abstract

Growth and histology of intestine and liver of carp fed diets with or devoid of fish meal (FM) was studied. Carp were fed four experimental diets formulated to contain 38% protein for 90 days. FM was incorporated at 30% in feed A, 15% in feed B and C, and was completely replaced with a mixture of plant proteins in feed D. Feed C and D were supplemented with methionine and lysine. The results showed that carp fed feed D had the lowest weight gain, length and height compared to the other three diets, whereas no differences were observed between A, B and C for the measured morphometric parameters. Inclusion of methionine and lysine tended to improve SGR of carp fed feed C compared to those fed feed B, but growth rate was lower than carp fed feed A. FCR differed for nearly 90 % between the FM rich and solely plant protein diet. No major pathological changes were recorded. At the end of the study shortening of intestinal folds' length was found for all groups, except for fish fed feed D. The height of enterocytes was significantly lower for carp fed diet D compared to other diets. According to the results obtained the best diet is feed A, but feed C with 15% FM and added methionine and lysine represents an acceptable replacement due to its lower price and effect on growth that are the most similar to feed A.

Effect of dietary fish meal replacement by poultry by-product meal on muscle fatty acid composition and liver histology of fry of Nile tilapia, Oreochromis niloticus (Actinopterygii: Perciformes: Cichlidae)

“…Tilapia are omnivorous fi sh, so they do not require high inclusion rate of FM, and do need greater amounts of Σ n-6 PUFA than Σ n-3 PUFA in their diets (Takeuchi et al 2002, Gümüş andErdogan 2010). Evaluation of histological structure of fi sh liver is important to assessing the effects of various nutrient feedstuffs that use raw materials in diet (Caballero et al 2004, Rašković et al 2011, Marković et al 2012, Hu et al 2013. In the presently reported study, the liver of fi sh fed the experimental diets demonstrated normal-shaped hepatocytes but, lower lipid ac- Glencross et al (2004) reported that small lipid droplet formation in the liver of rainbow trout was observed, which may in part be related to the poorer energy retention of a diet that included high levels of yellow lupin kernel meal.…”

Effect of different feed ingredients on digestive enzymes activity and on the histology of liver and intestine in Labeo rohita Hamilton, 1822

“…10). Markovic et al (2012) observed increased leucocytes infiltration in the epithelium often accompanied by increased mucous production when carp was fed with plant origin feed. Baeverfjord and Krogdahl (1996) and Uran et al (2008) reported enteritis which involved inflammatory infiltrate in fish intestine fed with soybean meal.…”

“…These samples were immediately fixed in 10% neutral buffered formalin and processed following standard histological procedures i.e., dehydrated in ascending series of ethanol concentrations, and embedded in paraffin. Tissue sections were cut at 4-5 μm, stained with hematoxylin and eosin (Humason, 1979;Markovic et al, 2012).…”

PENGARUH LIMBAH CAIR PERAWATAN CANDI BOROBUDUR TERHADAP FISIOLOGIS IKAN MAS (Cyprinus caprio)

“…ini dapat disebabkan oleh racun, infeksi, ataupun trauma sel (Raskovic, 2010). Kematian sel seperti ini akan menyebabkan fungsi hati menjadi menurun, proses detoksifikasi racun yang ada dalam tubuh akan semakin terhambat yang kemudian dapat menyebabkan ikan menjadi keracunan (Ayotunde, 2011 (Lewis, 1986).…”