m6A mRNA methylation analysis provides novel insights into heat stress responses in the liver tissue of sheep

Lu, Zengkui; Liu, Jianbin; Yuan, Chao; Jin, Meilin; Quan, Kai; Chu, Mingxing; Wei, Caihong

doi:10.1016/j.ygeno.2020.09.038

Cited by 21 publications

(16 citation statements)

References 58 publications

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“…Thus, the observed difference in THY would be considered a physiological adaptation to the change in environmental conditions to lower heat production and regulate heat balance [ 14 , 40 , 42 ]. This result is consistent with those found with other sheep breeds subjected to high heat load conditions [ 22 , 43 , 44 ].…”

Section: Discussionsupporting

confidence: 93%

“…There were no significant changes in COR concentration during the first 4 h of return to thermoneutral conditions (18 °C), but over the next 9 days, the COR level progressively returned to the pre-exposure level of 30 µg/L [ 50 ]. The reduction in COR concentration with long-term heat stress is beneficial to the body by lowering the metabolic rate and heat production, thus minimizing or preventing tissue damage [ 22 ].…”

Section: Discussionmentioning

confidence: 99%

“…Based primarily on rectal temperature and respiration rate, it was concluded that resilience to high heat load index conditions ranked St. Croix > Katahdin > Dorper, with lowest variability among individuals for St. Croix and greatest for Katahdin. These findings presumably relate to different phenotypic and morphological characteristics including skin and coat color, coat type, fat deposition, sweat gland number, and body conformation that influence responses to high HLI in functions such as catabolic and anabolic hormones, metabolic rates, nutrient partitioning, and molecular and gene modifications, which could be reflected in the blood biochemical profile [ 22 , 23 ]. Hence, levels of some hemato-biochemical constituents could be important response indicators of thermal challenge in sheep [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

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Effects of High Heat Load Conditions on Blood Constituent Concentrations in Dorper, Katahdin, and St. Croix Sheep from Different Regions of the USA

Tadesse

Patra

Puchała

et al. 2022

Animals

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Forty-six Dorper (DOR), 46 Katahdin (KAT), and 43 St. Croix (STC) female sheep (initial body weight of 58, 59, and 46 kg, respectively, SEM = 1.75; 3.3 ± 0.18 years of age, 2.6–3.7), derived from 45 commercial farms in four regions of the USA (Midwest, Northwest, Southeast, and central Texas), were used to evaluate responses in blood constituent concentrations to increasing heat load index (HLI) conditions. There were four sequential 2 weeks periods with target HLI during day/nighttime of 70/70 (thermoneutral zone conditions), 85/70, 90/77, and 95/81 in period 1, 2, 3 and 4, respectively. A 50% concentrate pelletized diet was fed at 53.3 g dry matter/kg body weight0.75. The analysis of most constituents was for samples collected on the last day of the second week of each period at 13:00 h; samples for cortisol, thyroxine, and heat shock protein were collected in week 2 and 8. Previously, it was noted that resilience to high HLI conditions was greatest for STC, lowest for DOR, and intermediate for KAT. There were few effects of region. Other than hemoglobin concentration, there were no interactions between breed and period. Blood oxygen concentration was greatest (p < 0.05) among breeds for STC (5.07, 5.20, and 5.53 mmol/L for DOR, KAT, and STC, respectively; SEM = 0.114) and differed among periods (4.92, 5.26, 5.36, and 5.52 mmol/L for period 1, 2, 3, and 4, respectively; SEM = 0.093). There were breed differences (i.e., main effects; p < 0.05) in glucose (50.0, 52.6, and 52.1 mg/dL; SEM = 0.76), urea nitrogen (17.2, 17.3, and 19.4 mg/dL; SEM = 0.33), creatinine (0.991, 0.862, and 0.802 mg/dL; SEM = 0.0151), total protein (6.50, 6.68, and 6.95 g/l; SEM = 0.017), triglycerides (28.4, 29.1, and 23.5 mg/dL; SEM = 0.87), and cortisol (6.30, 8.79, and 6.22 ng/mL for DOR, KAT, and STC, respectively; SEM = 0.596). Differences among periods (p < 0.05) were observed for lactate (27.9, 25.3, 27.8, and 24.0 mg/dL; SEM = 0.99), creatinine (0.839, 0.913, 0.871, and 0.917 mg/dL; SEM = 0.0128), total protein (6.94, 6.66, 6.60, and 6.65 g/l; SEM = 0.094), and cholesterol (60.2, 56.5, 58.3, and 57.6 mg/dL for period 1, 2, 3, and 4, respectively; SEM = 1.26). In addition, the concentration of cortisol (7.62 and 6.59 ng/mL; SEM = 0.404), thyroxine (5.83 and 5.00 µg/dL; SEM = 0.140), and heat shock protein (136 and 146 ng/mL for week 2 and 8, respectively; SEM = 4.0) differed between weeks (p < 0.05). In conclusion, the lack of interaction between breed and period with different HLI conditions suggests that levels of these blood constituents were not highly related to resilience to high HLI.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of High Heat Load Conditions on Blood Constituent Concentrations in Dorper, Katahdin, and St. Croix Sheep from Different Regions of the USA

Tadesse

Patra

Puchała

et al. 2022

Animals

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“…In this study, we identified 27,292 m6A peaks in OVA-induced lung tissues. Most of these m6A peaks were distributed in the 3' UTR and exon regions, which is inconsistent with the previously reported peak distribution trend in mouse liver ( 46 ), sheep liver ( 47 ), rat heart ( 48 ), and human tumor tissue ( 49 , 50 ). We considered that such inconsistencies may be related to different species, tissues, and conditions.…”

Section: Discussioncontrasting

confidence: 99%

N6-Methyladenosine Methylomic Landscape of Lung Tissues in Murine Acute Allergic Asthma

et al. 2021

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Allergic asthma is well known as a common respiratory disorder comprising an allergic inflammatory nature and excessive immune characteristic. N6-methyladenosine (m6A) methylation is an RNA epigenetic modification that post-transcriptionally regulates gene expression and function by affecting the RNA fate. Currently, m6A methylation is gaining attention as a mechanism of immunoregulation. However, whether m6A methylation engages the pathological process of asthma remains uncertain. Here, we present the m6A methylomic landscape in the lung tissues of ovalbumin-induced acute asthma mice using MeRIP-seq and RNA-seq. We identified 353 hypermethylated m6A peaks within 329 messenger RNAs (mRNAs) and 150 hypomethylated m6A peaks within 143 mRNAs in the lung tissues of asthmatic mice. These differentially methylated mRNAs were found to be involved in several immune function-relevant signaling pathways. In addition, we predicted 25 RNA-binding proteins that recognize the differentially methylated peak sites by exploring public databases, and the roles of these proteins are mostly related to mRNA biogenesis and metabolism. To further investigate the expression levels of the differentially methylated genes, we performed combined analysis of the m6A methylome and transcriptome data and identified 127 hypermethylated mRNAs (107 high and 20 low expression) and 43 hypomethylated mRNAs with differential expressions (9 high and 34 low expression). Of these, there are a list of mRNAs involved in immune function and regulation. The present results highlight the essential role of m6A methylation in the pathogenesis of asthma.

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“…Many studies related to m6A have been carried out in mammals. For example, 8,306 m6A peaks related to 2,697 genes were detected in the sheep heat stress group, and 12,958 m6A peaks related to 5,494 genes were detected in the control group ( 40 ). In the present study, 6,413 different peaks were detected, of which 521 were significantly upregulated and 1,231 were significantly downregulated.…”

Section: Discussionmentioning

confidence: 99%

N6-Methyladenosine Methylation Analysis of Long Noncoding RNAs and mRNAs in IPEC-J2 Cells Treated With Clostridium perfringens beta2 Toxin

Yang

Zhang

et al. 2021

Front. Immunol.

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BackgroundThe n6-methyladenosine (m6A) modification is present widely in mRNAs and long non-coding RNAs (lncRNAs), and is related to the occurrence and development of certain diseases. However, the role of m6A methylation in Clostridium perfringens type C infectious diarrhea remains unclear.MethodsHere, we treated intestinal porcine jejunum epithelial cells (IPEC-J2 cells) with Clostridium perfringens beta2 (CPB2) toxin to construct an in vitro model of Clostridium perfringens type C (C. perfringens type C) infectious diarrhea, and then used methylated RNA immunoprecipitation sequencing (MeRIP-seq) and RNA sequencing (RNA-seq) to identify the methylation profiles of mRNAs and lncRNAs in IPEC-J2 cells.ResultsWe identified 6,413 peaks, representing 5,825 m6A-modified mRNAs and 433 modified lncRNAs, of which 4,356 m6A modified mRNAs and 221 m6A modified lncRNAs were significantly differential expressed between the control group and CPB2 group. The motif GGACU was enriched significantly in both the control group and the CPB2 group. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation analysis showed that the differentially methylated modified mRNAs were mainly enriched in Hippo signaling pathway and Wnt signaling pathway. In addition, the target genes of the differentially m6A modified lncRNAs were related to defense response to virus and immune response. For example, ENSSSCG00000042575, ENSSSCG00000048701 and ENSSSCG00000048785 might regulate the defense response to virus, immune and inflammatory response to resist the harmful effects of viruses on cells.ConclusionIn summary, this study established the m6A transcription profile of mRNAs and lncRNAs in IPEC-J2 cells treated by CPB2 toxin. Further analysis showed that m6A-modified RNAs were related to defense against viruses and immune response after CPB2 toxin treatment of the cells. Threem6A-modified lncRNAs, ENSSSCG00000042575, ENSSSCG00000048785 and ENSSSCG00000048701, were most likely to play a key role in CPB2 toxin-treated IPEC-J2 cells. The results provide a theoretical basis for further research on the role of m6A modification in piglet diarrhea.

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m6A mRNA methylation analysis provides novel insights into heat stress responses in the liver tissue of sheep

Cited by 21 publications

References 58 publications

Effects of High Heat Load Conditions on Blood Constituent Concentrations in Dorper, Katahdin, and St. Croix Sheep from Different Regions of the USA

Effects of High Heat Load Conditions on Blood Constituent Concentrations in Dorper, Katahdin, and St. Croix Sheep from Different Regions of the USA

N6-Methyladenosine Methylomic Landscape of Lung Tissues in Murine Acute Allergic Asthma

N6-Methyladenosine Methylation Analysis of Long Noncoding RNAs and mRNAs in IPEC-J2 Cells Treated With Clostridium perfringens beta2 Toxin

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