M17 leucine aminopeptidase of the human malaria parasite Plasmodium vivax

Lee, Jung-Yub; Song, Sumin; Seok, Ji-Woong; Jha, Bijay Kumar; Han, Eun‐Taek; Song, Hyun-Ouk; Yu, Hak Sun; Hong, Yeonchul; Kong, Hyun-Hee; Chung, Dong‐Il

doi:10.1016/j.molbiopara.2009.11.003

Cited by 26 publications

(21 citation statements)

References 14 publications

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“…Despite the cytosolic nature of the majority of the characterized members of the M17 peptide family, these peptidases were already described as important in digestion in a series of organisms. Interestingly, M17 peptidases are necessary for hemoglobin digestion in the parasites Plasmodium falciparum and P. vivax (Lee et al, 2010 ; Skinner-Adams et al, 2010 ), Schistosoma mansoni and S. japonicum (McCarthy et al, 2004 ), in the tick Haemaphysalis longicornis (Hatta et al, 2006 ) and in the mite Psoroptes spp. (Nisbet and Billingsley, 2002 ).…”

Section: Discussionmentioning

confidence: 99%

Genome Wide Mapping of Peptidases in Rhodnius prolixus: Identification of Protease Gene Duplications, Horizontally Transferred Proteases and Analysis of Peptidase A1 Structures, with Considerations on Their Role in the Evolution of Hematophagy in Triatominae

et al. 2017

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Triatominae is a subfamily of the order Hemiptera whose species are able to feed in the vertebrate blood (i.e., hematophagy). This feeding behavior presents a great physiological challenge to insects, especially in Hemipteran species with a digestion performed by lysosomal-like cathepsins instead of the more common trypsin-like enzymes. With the aim of having a deeper understanding of protease involvement in the evolutionary adaptation for hematophagy in Hemipterans, we screened peptidases in the Rhodnius prolixus genome and characterized them using common blast (NCBI) and conserved domain analyses (HMMER/blast manager software, FAT, plus PFAM database). We compared the results with available sequences from other hemipteran species and with 18 arthropod genomes present in the MEROPS database. Rhodnius prolixus contains at least 433 protease coding genes, belonging to 71 protease families. Seven peptidase families in R. prolixus presented higher gene numbers when compared to other arthropod genomes. Further analysis indicated that a gene expansion of the protease family A1 (Eukaryotic aspartyl protease, PF00026) might have played a major role in the adaptation to hematophagy since most of these peptidase genes seem to be recently acquired, are expressed in the gut and present putative secretory pathway signal peptides. Besides that, most R. prolixus A1 peptidases showed high frequencies of basic residues at the protein surface, a typical structural signature of Cathepsin D-like proteins. Other peptidase families expanded in R. prolixus (i.e., C2 and M17) also presented significant differences between hematophagous (higher number of peptidases) and non-hematophagous species. This study also provides evidence for gene acquisition from microorganisms in some peptidase families in R. prolixus: (1) family M74 (murein endopeptidase), (2) family S29 (Hepatitis C virus NS3 protease), and (3) family S24 (repressor LexA). This study revealed new targets for studying the adaptation of these insects for digestion of blood meals and their competence as vectors of Chagas disease.

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Section: Discussionmentioning

confidence: 99%

Genome Wide Mapping of Peptidases in Rhodnius prolixus: Identification of Protease Gene Duplications, Horizontally Transferred Proteases and Analysis of Peptidase A1 Structures, with Considerations on Their Role in the Evolution of Hematophagy in Triatominae

et al. 2017

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“…LAPs were previously identified in the digestive organs of blood-feeding parasites including ticks [ 155 ], schistosomes [ 156 ], and Plasmodium spp. [ 157 ] and were found to be involved in the final stage of hemoglobin digestion. Because hemoglobin could also represent part of the L. sericata diet, we can speculate that L. sericata LAPs similarly are required for hemoglobin digestion.…”

Section: Resultsmentioning

confidence: 99%

Next Generation Sequencing Identifies Five Major Classes of Potentially Therapeutic Enzymes Secreted byLucilia sericataMedical Maggots

Franta

Vogel

Lehmann

et al. 2016

BioMed Research International

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Lucilia sericata larvae are used as an alternative treatment for recalcitrant and chronic wounds. Their excretions/secretions contain molecules that facilitate tissue debridement, disinfect, or accelerate wound healing and have therefore been recognized as a potential source of novel therapeutic compounds. Among the substances present in excretions/secretions various peptidase activities promoting the wound healing processes have been detected but the peptidases responsible for these activities remain mostly unidentified. To explore these enzymes we applied next generation sequencing to analyze the transcriptomes of different maggot tissues (salivary glands, gut, and crop) associated with the production of excretions/secretions and/or with digestion as well as the rest of the larval body. As a result we obtained more than 123.8 million paired-end reads, which were assembled de novo using Trinity and Oases assemblers, yielding 41,421 contigs with an N50 contig length of 2.22 kb and a total length of 67.79 Mb. BLASTp analysis against the MEROPS database identified 1729 contigs in 577 clusters encoding five peptidase classes (serine, cysteine, aspartic, threonine, and metallopeptidases), which were assigned to 26 clans, 48 families, and 185 peptidase species. The individual enzymes were differentially expressed among maggot tissues and included peptidase activities related to the therapeutic effects of maggot excretions/secretions.

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“…Leucine is a virus detected in endemic diseases such as malaria [6], dengue fever [7], typhoid [8], serious cough, dyspnea, papule and so on [9,10]. Those diseases are infected by parasite and host animals, and cause muscle pain.…”

Section: Resultsmentioning

confidence: 99%

Data mining and analysis of bacillus virus

Kim

Moon

et al. 2016

MATEC Web Conf.

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Abstract. Anthrax-can be found naturally in soil and commonly affects domestic and wild animals around the world. Bacillus anthracis is mostly common viruses of Anthrax. There are some more occurred in similar DNA sequences: 3 Bacillus Viruses: Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis. This is a report about analyzing the similarities between the Bacillus viruses by investigating the frequency of amino acid and finding the difference between those three viruses based on the gene. Those diseases are infected by parasite and host animals, and cause muscle pain. Therefore, we can conclude that Leucine is a protein that plays a significant role in causing muscle pain. Secondly, In Analysis of decision tree, there are only little differences between each classes. The classes represent positions that include representative protein. The very position that windows mention is their difference.

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M17 leucine aminopeptidase of the human malaria parasite Plasmodium vivax

Cited by 26 publications

References 14 publications

Genome Wide Mapping of Peptidases in Rhodnius prolixus: Identification of Protease Gene Duplications, Horizontally Transferred Proteases and Analysis of Peptidase A1 Structures, with Considerations on Their Role in the Evolution of Hematophagy in Triatominae

Genome Wide Mapping of Peptidases in Rhodnius prolixus: Identification of Protease Gene Duplications, Horizontally Transferred Proteases and Analysis of Peptidase A1 Structures, with Considerations on Their Role in the Evolution of Hematophagy in Triatominae

Next Generation Sequencing Identifies Five Major Classes of Potentially Therapeutic Enzymes Secreted byLucilia sericataMedical Maggots

Data mining and analysis of bacillus virus

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