M1675 Intestinal Glucose-Induced Phosphorylation of Calcium-Calmodulin Kinase II (CaMKII) in 5-HT-Secreting Enterochromaffin Cells, Myenteric Neurons and Vagal Afferents in the Rat

Sharp, James W.; Dulake, Michelle; Vincent, Kimberly M.; Raybould, Helen E.

doi:10.1016/s0016-5085(08)61845-4

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“…Previous work has demonstrated that 5-HT 3 -receptor signaling mediates glucose-induced inhibition of gastric emptying, and is inhibitable by ondansetron. 52,53 Recent work has also identified a large subpopulation of α -gustducin-expressing enteroendocrine cells throughout the small intestine that colocalize with 5-HT. 54 Given vagal afferents also express 5-HT 3 -receptors, 52 this supports the concept that taste-receptors may indirectly stimulate vagal afferent firing.…”

Section: Discussionmentioning

confidence: 99%

Rapid Upregulation of Sodium-Glucose Transporter SGLT1 in Response to Intestinal Sweet Taste Stimulation

Stearns¹,

Balakrishnan²,

Rhoads³

et al. 2010

Annals of Surgery

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Objective-We set out to examine the short-term regulation of the intestinal sodium/glucose cotransporter SGLT1 by its substrate glucose and sweet taste analogs.Summary Background Data-Intestinal SGLT1 is a putative target for antidiabetic therapy; however, its physiological regulation is incompletely understood, limiting its application as a pharmacological target. While it is clearly regulated by dietary composition over a period of days, its short-term regulation by nutrients is unknown.Methods-Sprague-Dawley rats were anesthetized, and the duodenum cannulated. D-glucose, Dfructose, saccharin, D-mannitol, and water were infused for 3 hours, before harvest of proximal jejunum for SGLT1 analysis with Western blotting and quantitative polymerase chain reaction. In further experiments, the receptor region was identified by D-glucose infusion of isolated regions. Lastly, the vagus was de-afferented with capsaicin, and 5HT3-receptor activation of vagal afferents inhibited using ondansetron, before repeating experiments using water or D-glucose infusion.

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