M. tuberculosis infection and antigen specific cytokine response in healthcare workers frequently exposed to tuberculosis

Abstract

Tuberculosis (TB) is the leading cause of death due to an infectious agent, but only a small fraction of those infected develop the disease. Cytokines are involved in the mediation and regulation of immunity, and their secretion patterns may reflect the infection status. To increase our understanding of immune response to M . tuberculosis infection, we conducted a cross-sectional study investigating M . tuberculosis infection status and comparing the release profiles of cytokines GM-CSF, IFN-γ, IL-1β, IL-10, IL-12 (p70), IL-2, IL-4, IL-5, IL-6, IL-8, TNF-α, in community controls (CCs) and healthy healthcare workers (HCWs) highly exposed to TB. Among HCWs and CCs, the probability of latent M . tuberculosis (LTB + ) infection was respectively 5.4 (p = 0.002) and 3.4 (p = 0.006) times higher in men than women. The odds ratio of LTB infection was 4 times higher among HCWs in direct contact with active TB patients than other HCW (p = 0.01). Whole blood supernatant cytokine responses to M . tuberculosis antigens showed differential pro-inflammatory responses between HCWs and CCs. CCs LTB− had higher IL-1β responses than HCWs LTB− (p = 0.002). HCWs LTB+ had significantly higher IL-8 responses to M . tuberculosis antigens than HCWs LTB− (p = 0.003) and CCs LTB− (p = 0.015). HCWs LTB+/− showed weak but positive TNF-α responses to M . tuberculosis antigen stimulation compared to CCs LTB+/− (p ≤ 0.015). Looking at T-helper (1 and 2) responses, HCWs LTB+ and CCs LTB+ had significantly higher IFN-γ and IL-2 responses compared to HCWs LTB− and CCs LTB− (p < [0.0001–0.003]). Also, TB antigen induced IL-5 secretion was significantly higher in HCWs LTB+ and CCs LTB+ than in non-infected CCs LTB− (p < [0.005–0.04]). M . tuberculosis antigen specific responses in HCWs LTB+ varied based on active TB exposure gradient. HCWs LTB+ who were highly exposed to active TB (≥3 hours per day) had significantly higher IFN-γ and IL-8 responses (p ≤ 0.02) than HCWs LTB+ not in direct contact with active TB patients. HCWs LTB+ working with active TB patients for 5 to 31 years had a significantly enhanced secretion of proinflammatory cytokines (GM-CSF, IFN-γ, IL-1β, IL-2, IL-6, IL-8, IL-12p70, TNF-α) compared to HCWs LTB− (p < [0.0001–0.01]). Secretion of anti-inflammatory/T...

Evidence and implications of pre‐existing humoral cross‐reactive immunity to SARS‐CoV‐2

“…The participants were initially screened for latent TB infection (QuantiFERON‐TB Gold), HIV, HVB (VIKIA HBs Ag bioMerieux), HCV (VIKIA anti HCV, bioMerieux) and syphilis. Clinical histories including Bacillus Calmette–Guérin vaccination and chronic diseases were recorded from all subjects 2 …”

Co-Administration of Anticancer Candidate MK-2206 Enhances the Efficacy of BCG Vaccine Against Mycobacterium tuberculosis in Mice and Guinea Pigs

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Challenges and the Way forward in Diagnosis and Treatment of Tuberculosis Infection

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