m<sup>6</sup>A‐mediated regulation of crop development and stress responses

Zhou, Leilei; Gao, Guangtong; Tang, Renkun; Wang, Weihao; Wang, Yuying; Tian, Shiping; Qin, Guozheng

doi:10.1111/pbi.13792

Cited by 42 publications

(22 citation statements)

References 89 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In plants, transcript m 6 A marks are preferentially deposited around stop codons and within 3′ untranslated regions (3′ UTRs). This m 6 A distribution appears to be evolutionarily conserved in crops and divergent plant species (Miao et al, 2022; Zhou et al, 2022). Consistent with these reports, approximately 50% of m 6 A peaks identified in both healthy and virus‐infected samples were located in host transcript 3′ UTRs (Figure 2c).…”

Section: Figurementioning

confidence: 99%

See 1 more Smart Citation

AlkB RNA demethylase homologues and N⁶‐methyladenosine are involved in Potyvirus infection

Yue

Yao

Sun

et al. 2022

Molecular Plant Pathology

View full text Add to dashboard Cite

Proteins of the alkylation B (AlkB) superfamily show RNA demethylase activity removing methyl adducts from N 6 -methyladenosine (m 6 A). m 6 A is a reversible epigenetic mark of RNA that regulates human virus replication but has unclear roles in plant virus infection. We focused on Potyvirus-the largest genus of plant RNA viruses-and report here the identification of AlkB domains within P1 of endive necrotic mosaic virus (ENMV) and an additional virus of a putative novel species within Potyvirus. We show that Nicotiana benthamiana m 6 A levels are reduced by infection of plum pox virus (PPV) and potato virus Y (PVY). The two potyviruses lack AlkB and the results suggest a general involvement of RNA methylation in potyvirus infection and evolution. Methylated RNA immunoprecipitation sequencing of virus-infected samples showed that m 6 A peaks are enriched in plant transcript 3′ untranslated regions and in discrete internal and 3′ terminal regions of PPV and PVY genomes. Down-regulation of N. benthamiana AlkB homologues of the plant-specific ALKBH9 clade caused a significant decrease in PPV and PVY accumulation. In summary, our study provides evolutionary and experimental evidence that supports the m 6 A implication and the proviral roles of AlkB homologues in Potyvirus infection.

show abstract

Section: Figurementioning

confidence: 99%

“…In plants, m 6 A functionally modulates a variety of processes including development and responses to biotic and abiotic stress (Zhou et al, 2022). Very recent reports support its involvement in plant–virus interaction (Yue et al, 2022).…”

Section: Figurementioning

confidence: 99%

AlkB RNA demethylase homologues and N⁶‐methyladenosine are involved in Potyvirus infection

Yue

Yao

Sun

et al. 2022

Molecular Plant Pathology

View full text Add to dashboard Cite

show abstract

“…Generally, fruit at low maturity are more resistant to pathogens, but gradually become susceptible when the ripening program is triggered [ 88 ]. Accompanied with substantial biochemical and physiological variations, fruit ripening is coordinated by complex signaling events at transcriptional, post-transcriptional and epigenetic levels [ 20 , 89 ]. As revealed by integrative transcriptomic and metabolomic analysis on 580 introgression lines, S. pennellii introgressions resulted in the variations for a bulk of transcripts and metabolites in ripening-related processes [ 8 ].…”

Section: S Lycopersicum Is a Classic Model Species For Studi...mentioning

confidence: 99%

Solanum lycopersicum, a Model Plant for the Studies in Developmental Biology, Stress Biology and Food Science

Liu

Chen

et al. 2022

Foods

Self Cite

View full text Add to dashboard Cite

Fruits, vegetables and other plant-derived foods contribute important ingredients for human diets, and are thus favored by consumers worldwide. Among these horticultural crops, tomato belongs to the Solanaceae family, ranks only secondary to potato (S. tuberosum L.) in yields and is widely cultivated for fresh fruit and processed foods owing to its abundant nutritional constituents (including vitamins, dietary fibers, antioxidants and pigments). Aside from its important economic and nutritional values, tomato is also well received as a model species for the studies on many fundamental biological events, including regulations on flowering, shoot apical meristem maintenance, fruit ripening, as well as responses to abiotic and biotic stresses (such as light, salinity, temperature and various pathogens). Moreover, tomato also provides abundant health-promoting secondary metabolites (flavonoids, phenolics, alkaloids, etc.), making it an excellent source and experimental system for investigating nutrient biosynthesis and availability in food science. Here, we summarize some latest results on these aspects, which may provide some references for further investigations on developmental biology, stress signaling and food science.

show abstract

“…The dCas9-SunTag-VP64 system targeting SUPERMAN and FWA promoters for DRM2 methylation and N6-methyleadenosine (m6A) modification are the most frequently used types of regulatory mechanisms for epigenome editing in eukaryotes ( Papikian et al., 2019 ; Yue et al., 2019 ). The CRISPR-based m6A editing system comprising m6A enzymes (writers or erasers) fused to the Cas13 protein has been recently demonstrated in Arabidopsis , tobacco, rice and tomato plants ( Zheng et al., 2020 ; Zhou et al., 2022a ). Thus, the present sketch reveals that this technology is proving to be a great asset to agriculture in transforming the development of crops to be more tolerant of biotic/abiotic stresses and climate change.…”

Section: Crispr Technology For Engineering Thermo−tolerant Crop Plantsmentioning

confidence: 99%

Molecular insights into mechanisms underlying thermo-tolerance in tomato

et al. 2022

View full text Add to dashboard Cite

Plant productivity is being seriously compromised by climate-change-induced temperature extremities. Agriculture and food safety are threatened due to global warming, and in many cases the negative impacts have already begun. Heat stress leads to significant losses in yield due to changes in growth pattern, plant phonologies, sensitivity to pests, flowering, grain filling, maturity period shrinkage, and senescence. Tomato is the second most important vegetable crop. It is very sensitive to heat stress and thus, yield losses in tomato due to heat stress could affect food and nutritional security. Tomato plants respond to heat stress with a variety of cellular, physiological, and molecular responses, beginning with the early heat sensing, followed by signal transduction, antioxidant defense, osmolyte synthesis and regulated gene expression. Recent findings suggest that specific plant organs are extremely sensitive to heat compared to the entire plant, redirecting the research more towards generative tissues. This is because, during sexual reproduction, developing pollens are the most sensitive to heat. Often, just a few degrees of temperature elevation during pollen development can have a negative effect on crop production. Furthermore, recent research has discovered certain genetic and epigenetic mechanisms playing key role in thermo-tolerance and have defined new directions for tomato heat stress response (HSR). Present challenges are to increase the understanding of molecular mechanisms underlying HS, and to identify superior genotypes with more tolerance to extreme temperatures. Several metabolites, genes, heat shock factors (HSFs) and microRNAs work together to regulate the plant HSR. The present review provides an insight into molecular mechanisms of heat tolerance and current knowledge of genetic and epigenetic control of heat-tolerance in tomato for sustainable agriculture in the future. The information will significantly contribute to improve breeding programs for development of heat tolerant cultivars.

show abstract

m⁶A‐mediated regulation of crop development and stress responses

Cited by 42 publications

References 89 publications

AlkB RNA demethylase homologues and N⁶‐methyladenosine are involved in Potyvirus infection

AlkB RNA demethylase homologues and N⁶‐methyladenosine are involved in Potyvirus infection

Solanum lycopersicum, a Model Plant for the Studies in Developmental Biology, Stress Biology and Food Science

Molecular insights into mechanisms underlying thermo-tolerance in tomato

Contact Info

Product

Resources

About

m6A‐mediated regulation of crop development and stress responses

Cited by 42 publications

References 89 publications

AlkB RNA demethylase homologues and N6‐methyladenosine are involved in Potyvirus infection

AlkB RNA demethylase homologues and N6‐methyladenosine are involved in Potyvirus infection

Solanum lycopersicum, a Model Plant for the Studies in Developmental Biology, Stress Biology and Food Science

Molecular insights into mechanisms underlying thermo-tolerance in tomato

Contact Info

Product

Resources

About

m⁶A‐mediated regulation of crop development and stress responses

AlkB RNA demethylase homologues and N⁶‐methyladenosine are involved in Potyvirus infection

AlkB RNA demethylase homologues and N⁶‐methyladenosine are involved in Potyvirus infection