M-specific reverse transcription loop-mediated isothermal amplification for detection of pandemic (H1N1) 2009 virus

Abstract

This is an accurate and fast analysis system suitable for general diagnostic laboratories with only limited equipment, e.g. first-line health care centre. This assay will help clinicians and public health officials to react effectively during an outbreak.

Multiplex Reverse-Transcription Loop-Mediated Isothermal Amplification Coupled with Cascade Invasive Reaction and Nanoparticle Hybridization for Subtyping of Influenza A Virus

“…Previous studies have shown that although multiplex nucleic acid detection tests have the advantage of high efficiency, due to the mutual interference of multiple primers, the analytic sensitivities of these assays tend to decrease. However, through the signal amplification effects of invader techniques and gold nanoparticle probe techniques, the sensitivity of the mRT-LAMP-IRNH assay established in this study was equivalent or even significantly increased as compared to general monoplex LAMP method23434445. In this study, the detection limit for A/H5 obtained by mRT-LAMP-IRNH was ten times higher than that obtained by real-time turbidity detection, indicating the cascade invasive reaction coupled with AuNPs hybridization increased the sensitivity of LAMP whose results were usually analyzed through turbidity.…”

Rapid Detection of Viruses Using Loop-Mediated Isothermal Amplification (LAMP): A Review

“…(WHO 2009a ) or by designing infl uenza A virus consensus RT-LAMP assay primers and performing the two RT-LAMP assays together. Based on above facts and data, there is an urgent need for the development of an easy, accurate, and simple method for the diagnosis of this novel pandemic virus (Lee et al 2010 ). Lee et al ( 2010 ) developed M-specifi c reverse transcription loop-mediated isothermal amplifi cation for detection of pandemic (H1N1) 2009 virus.…”

“…Based on above facts and data, there is an urgent need for the development of an easy, accurate, and simple method for the diagnosis of this novel pandemic virus (Lee et al 2010 ). Lee et al ( 2010 ) developed M-specifi c reverse transcription loop-mediated isothermal amplifi cation for detection of pandemic (H1N1) 2009 virus. Based on validation by virus isolation, the specifi city and sensitivity of this M-specifi c RT-LAMP assay were 100 % and 98.25 %, respectively.…”

“…LAMP method has been developed for detection of human viruses including chickenpox virus (Okamoto et al 2004 ); mumps virus (Yoshida et al 2008 ); Colorado tick fever virus Coltivirus; respiratory syncytial virus (Ushio et al 2005 ); fl avivirus causing West Nile fever ; enterovirus 71 ; human immunodefi ciency virus (HIV) causing acquired immune defi ciency syndrome (AIDS) (Hosaka et al 2009 ); pandemic (H1N1) 2009 virus (Lee et al 2010 ); cytomegalovirus causing cytomegalovirus inclusion disease (Reddy et al 2010 ); chikungunya virus (Parida et al 2007 ); Japanese encephalitis virus (Parida et al 2006 ); herpes simplex virus type 2 causing genital herpes (Enomoto et al 2005 ); adult T-cell leukemia and hairy cell leukemia viruses; human T-cell lymphotropic-1 and human T-cell lymphotropic-2 retroviruses (Komiyama et al 2009 ); foot-andmouth disease virus (Chen et al 2011 ); EpsteinBarr virus (EBV) (Iwata et al 2006 ), infl uenza A and B viruses causing infl uenza (Ito et al 2006 ); Ebola virus (Kurosaki et al 2007 ); human papilloma viruses type 6, 11, 16 and 18 (Hagiwara et al 2007 ); and HIV-1 virus (Curtis et al 2009 ). Mumps virus was circulating throughout the world, but in the United States the acceptance of MMR combined vaccine against measles, mumps, and rubella has reduced the number of patients suffering from mumps (Centers for Disease Control and Prevention 1998 ;Peltola et al 1994 ).…”

Molecular Assays for the Detection and Characterization of Respiratory Viruses

“…87,88 LAMP has successfully been applied to the rapid and real-time detection of common and emerging respiratory viral pathogens, including SARS-CoV 85,89 and the recently emerged novel A/H1N1. [90][91][92] RT-LAMP was used to detect Flu-A viruses covering H1 to H3. 77 Another similar RT-LAMP assay was described more recently that detects Flu-A virus H1 and H3 subtype strains and Flu-B virus strains.…”