M · H2I, a multispecific 5C-DNA methyltransferase encoded by Bacillus amyloliquefaciens phage H2

Environmentally Selected Aphid Variants in Clonality Context Display Differential Patterns of Methylation in the Genome

“…The secondary endosymbionts are known to have a heavy load of transposases and shelter phages and plasmids [38,39]. Phages are known to carry genes expressing methylases, like the multispecific cytosine methylase, functioning at sites like GGCC, GCGC or GGA/TGCCT/AC [37,[40][41][42] and the secondary endosymbiont Candidatus hamiltonella is known to harbor the methylase Dcm that modifies the second cytosine of CCA/TGG in the bacterial genome [36]. Many of these sites were found in the aphid genome pull down of methylated fragments.…”

Organization of restriction-modification systems

“…Between the TRDs is a section that spans the interval between the sequences. Crossovers within this section have generated new R-M system specificities (24,66 6The CviJI methyltransferase is less specific than the endonuclease; it methylates RGCB, and possibly VGCB (103,104 (191), is distinct from the methyltransferase of the BamHI R-M system (82,86), and from the H2 m5C-methyltransferase from T.Trautner's group (192). An alternative start for the gene would make it 279 codons in length.…”

Bacteriophage Orphan DNA Methyltransferases: Insights from Their Bacterial Origin, Function, and Occurrence

“…1B). B. amyloliquefaciens phage H2 carries a gene that encodes a multispecific m5c methyltransferase that was found to share high sequence homology with its counterparts on the genomes of ø3T, SPR, and p11 s (58). Furthermore, the ability of these MTases to recognize more than a single target sequence may provide a particular phage with immunity to several R-M systems.…”