M Ficolin: Diffusion dynamics from Blood to Cerebrospinal Fluid

Abstract

INTRODUCTIONFicolins belong to the lectins family. They activate the complement system as an effect in defence of the carrier, and play a very important role in the innate immunity.Three kinds of human Ficolins were earlier described: L, H and M ones. The theory of molecular dissemination is the basis of the behavior's approach from these proteins that can be found in cerebrospinal fluid (CSF).OBJECTIVETo demonstrate through the dynamic of the dissemination from blood to CSF and its concentrations the different ways of molecular adding towards CSF and its action in this biological liquid.METHODS80 serum and CSF paired samples were obtained from control persons without neuroinflammation process and without oligoclonal IgG bands. M ficolin and albumin were quantified by ELISA and by an immunoturbidimetric analysis. Q albumin=CSF albumin/serum albumin and Q, M Ficolin=CSF M Ficolin/serum M ficolin was calculated.To calculate the different aggregation forms were determinate the inflection points of the frequency distribution of M ficolin vs. Q M Ficolin taking into account that the more complex structure should diffuse more than a most simple one.RESULTSSerum and CSF M ficolin did not follow a normal distribution according to Kolmogorov‐Smirnov test. Mean values of M ficolin in serum was higher than its mean content in CSF. CSF M ficolin concentration increased with the increase of Q albumin. M ficolin is not a brain‐derived protein. Three inflection points was determined. (Table 1 and figure 1). G‐1, G‐2, G‐3 described the subpopulation of samples diffusion limited by their molecular weight and its aggregation structure.CONCLUSIONM ficolin is a predominant blood‐derived protein and the diffusion from blood to CSF indicates three aggregation forms.This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.