M-DNA/Transition Metal Dichalcogenide Hybrid Structure-based Bio-FET sensor with Ultra-high Sensitivity

Abstract

Here, we report a high performance biosensor based on (i) a Cu2+-DNA/MoS2 hybrid structure and (ii) a field effect transistor, which we refer to as a bio-FET, presenting a high sensitivity of 1.7 × 103 A/A. This high sensitivity was achieved by using a DNA nanostructure with copper ions (Cu2+) that induced a positive polarity in the DNA (receptor). This strategy improved the detecting ability for doxorubicin-like molecules (target) that have a negative polarity. Very short distance between the biomolecules and the sensor surface was obtained without using a dielectric layer, contributing to the high sensitivity. We first investigated the effect of doxorubicin on DNA/MoS2 and Cu2+-DNA/MoS2 nanostructures using Raman spectroscopy and Kelvin force probe microscopy. Then, we analyzed the sensing mechanism and performance in DNA/MoS2- and Cu2+-DNA/MoS2-based bio-FETs by electrical measurements (ID-VG at various VD) for various concentrations of doxorubicin. Finally, successful operation of the Cu2+-DNA/MoS2 bio-FET was demonstrated for six cycles (each cycle consisted of four steps: 2 preparation steps, a sensing step, and an erasing step) with different doxorubicin concentrations. The bio-FET showed excellent reusability, which has not been achieved previously in 2D biosensors.

Modulation of Optical and Electrical Characteristics by Laterally Stretching DNAs on CVD-Grown Monolayers of MoS2

“…The averaged Raman spectra in Figure 3(e) show the redshift on both the E 1 2g and A 1g Raman modes in 1L-MoS 2 by 5 and 3 cm −1 , respectively, after DNA stretching. The red-shift of the E 1 2g mode for DNA/MoS 2 systems was previously observed [23,24] and the observed red-shift in the peak position of the E 1 2g Raman mode indicates the presence of tensile strain in 1L-MoS 2 with lateral stretching of DNAs, which amount to ∼1% according to previous empirical results [10,36]. The red-shift of A exciton PL peak and A 1g Raman mode in DNA-stretched 1L-MoS 2 can be caused by electron doping in 1L-MoS 2 [9,12].…”

“…While the deposition of DNA solution was previously used for DNA/TMD hybridization [23,24], here we laterally stretched strands of DNA molecules on 1L-TMDs. Figure 1 shows the schematic for stretching the DNA molecules on the glass substrate and MoS 2 surface.…”

“…While there have been many efforts to create hybrid structures of TMDs for enhanced physical and chemical characteristics [19][20][21][22], the hybridization of biomaterials and TMDs is relatively rare. Recently, ultra-high sensitivity fieldeffect transistor (FET) sensor using DNA-TMD system has been demonstrated [23], and DNA inducing doping effect on thick TMDs materials have been introduced [24,25]. Biomolecules and TMD materials hold significant potential for the development of extremely small devices with increasingly complex functionality, which could open various applications in bioelectronics.…”

Metallo-Curcumin-Conjugated DNA Complexes Induces Preferential Prostate Cancer Cells Cytotoxicity and Pause Growth of Bacterial Cells

“…The diverse potential structures of DNA molecules due to their inherent base-sequence programmability and self-assembly by hydrogen bonding between bases has resulted in DNA being widely recognized as one of the most prominent nanoengineering materials 8 – 10 . This technology is a fusion of various disciplines such as biology, physics, chemistry, and electronics, allowing us to use it in biomedicine and create a variety of functionalized devices and sensors 11 – 18 . Consequently, researchers are focusing on the construction of diverse shapes of DNA nanostructures for in vitro and in vivo medical applications (including DNA vehicles for drug delivery) as well as for conventional electromagnetic and optical devices and chemical and biological sensors.…”

Hydrothermal Synthesis of Cr2Se3 Hexagons for Sensitive and Low-level Detection of 4-Nitrophenol in Water

“…The synthesis of novel materials has received significant interest across many disciplines including analytical chemistry. In particular, the synthesis of transition metal chalcogenides has been the subject of considerable attention and derived materials have been widely utilized in supercapacitors 1 , solar cells 2 , batteries 3 , and sensors 4 due to their high energy density, long cycling stability, and excellent electrochemical and charge transfer properties 5 – 7 . Accordingly, transition metal chalcogenides such as metal sulfide, selenide, telluride, nitride, boride, and phosphide have also been widely prepared and employed in energy and sensor applications 8 – 11 .…”