M cells in the rabbit palatine tonsil: the distribution, spatial arrangement and membrane subdomains as defined by confocal lectin histochemistry

Gebert, Andreas

doi:10.1007/s004290050055

Cited by 16 publications

(13 citation statements)

References 20 publications

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“…Morphologically, Mcells described in the present study are similar, but not identical, to those described in sheep (Chen et al, 1991;Casteleyn et al, 2010). Tonsilar M-cells in rabbits have been identified through lectin or vimentin immunohistochemistry (Gebert, 1995(Gebert, , 1996(Gebert, , 1997. Recently, cytokeratin 18 was shown to be a marker of ileal dome M-cells in cattle .…”

Section: Discussionsupporting

confidence: 74%

Examination of the Reticular Epithelium of the Bovine Pharyngeal Tonsil

Palmer

Stasko

Waters

et al. 2011

The Anatomical Record

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The pharyngeal tonsil (adenoid), located at the posterior of the nasopharynx is ideally positioned to sample antigens passing through the nasal cavity or oral cavity. Entering antigens will first contact tonsilar epithelium. To better understand the cellular organization of this important epithelial layer, pharyngeal tonsils were collected from six, 7-month-old calves and examined by light microscopy, immunohistochemistry, and electron microscopy. Morphometric analysis showed that the epithelium overlying lymphoid follicles (reticular epithelium) contained significantly more B-cells, CD4þ, and CD11cþ cells than nonreticular epithelium. In contrast, nonreticular epithelium contained significantly more, c/d TCRþ cells than reticular epithelium. Scanning and transmission electron microscopy of reticular epithelium identified a heterogeneous population of epithelial cells, many of which displayed morphologic characteristics of M-cells. Moreover, putative M-cells were shown to possess the capacity for microparticle uptake. Bovine pharyngeal tonsilar reticular epithelium contains key immune cells, as well as M-cells; elements essential for antigen uptake, antigen processing, and initiation of immune responses. A better understanding of the morphology and function of tonsilar lymphoepithelium will strengthen our understanding of it's role in disease pathogenesis, and potential use as an induction site for mucosal immune responses to vaccination. Anat Rec, 294:1939Rec, 294: -1950Rec, 294: , 2011. V V C 2011 Wiley-Liss, Inc.

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Section: Discussionsupporting

confidence: 74%

Examination of the Reticular Epithelium of the Bovine Pharyngeal Tonsil

Palmer

Stasko

Waters

et al. 2011

The Anatomical Record

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“…The epithelium overlying the PP dome is called the follicleassociated epithelium (FAE) and contains the M cells specialized for the uptake of antigen. M cells have also been observed in the epithelium of other MALTs with a morphology similar to that of GALT M cells, for example in the palatine tonsils of rabbits [21].…”

Section: Inductive Sites Of the Mucosal Immune Systemmentioning

confidence: 85%

“…M cells outside the gut, such as in rabbit palatine tonsil epithelium, also have this characteristic shape with an invagination of the basolateral membrane forming a pocket containing lymphocytes. Gebert [21] found that the region of the basolateral membrane forming the pocket had a different lectin-binding pattern than the rest of the basolateral membrane, suggesting that the compostion of the membrane is not uniform. Apical alkaline phosphatase is less abundant, but esterase is more abundant in M cells than that of adjacent absorptive cells [25].…”

Section: Mucosa-associated Lymphoid Tissue Presence Of M Cell In Epitmentioning

confidence: 99%

The role of M cells in mucosal immunity

Hathaway¹,

Kraehenbuhl²

2000

CMLS, Cell. Mol. Life Sci.

109

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Mucosa-associated lymphoid tissue in the respiratory and digestive tracts are covered by a specialized epithelium, the follicle-associated epithelium, which includes M cells, which are specialized for the uptake and transcytosis of macromolecules and microorganisms. Following transcytosis, antigens are released to cells of the immune system in lymphoid aggregates beneath the epithelium where antigen processing and presentation and stimulation of specific B and T lymphocytes are achieved. Circulation of the lymphoid cells enables their homing to their original, and other, mucosal sites where they exert the effector function. Such a response may be dominated by secretory immunoglobulin A release and may include cytotoxic T lymphocyte action. Binding of particles to the apical M cell membrane may be nonspecific or due to specific interaction between molecules such as integrins and lectins. Exploiting the specific binding to M cells is an aim for mucosal vaccination, for example to increase the efficiency of uptake of an oral vaccine by its conjugation to an M-cell-specific molecule. Alternatively, an M-cell-specific live vector, such as attenuated Salmonella bacteria, may be used to deliver epitopes of other organisms. Mucosal vaccination efficiency may also be enhanced by a temporary increase in the number of M cells. Therefore, investigation of the properties and ontogeny of M cells must be pursued to allow the development of better mucosal vaccines for the future.

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“…In general, it is known that lectinlike adhesions on the outer membrane of various microorganisms are involved in the adherence. Recently, using confocal scanning microscopy, it was shown that M cells of the rabbit palatine tonsil bind the lectin derived from Ulex europeus (UEA-1) [8]. It has also been shown that the NALT M-cell lineage in hamsters is distinguished from other respiratory epithelial cells by the expression of glycoconjugates bearing terminal a(1-3)-linked galactose [9].…”

Section: Discussionmentioning

confidence: 99%

Evidence of M cells as portals of entry for antigens in the nasopharyngeal lymphoid tissue of humans

Fujimura¹

2000

Virchows Archiv

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The nasopharyngeal tonsils (adenoids) are prominent components of human nasal-associated lymphoid tissues (NALT). However, the role of the nasopharyngeal tonsils in antigen uptake for initiation of the mucosal immune response is unknown. The aims of this study were to describe the ultrastructure and function of the M cells of the human nasopharyngeal tonsils and to clarify their capacity for antigen uptake. Tissues obtained from eight patients undergoing adenectomy were examined by light and electron microscopy. Lymphoepithelium covers the nasopharyngeal lymphoid tissue and consists of ciliary epithelium, non-ciliary epithelial cells, M cells, goblet cells, and many intraepithelial lymphoid cells. M cells have irregular and broad cytoplasm-containing microvilli on their surface and small vesicles in their cytoplasm. Many lymphoid cells were enfolded by M cells. The uptake of horseradish peroxidase (HRP) in the tissue in organ culture was studied using histochemical techniques. Excised adenoid tissue was incubated in RPMI 1640 culture media with HRP for 10, 30, and 60 min. HRP which had adhered to the surface was taken up in vesicles and then transported in vesicles and tubules by M cells. The M cells of nasopharyngeal lymphoid tissue were ultrastructurally and functionally similar to those in human Peyer's patches and colonic lymphoid follicles. These findings indicate that NALT bears similarities to the gut-associated lymphoid tissue, and its antigen uptake capacity may be important for initiation of immunity in the upper aerodigestive tract.

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M cells in the rabbit palatine tonsil: the distribution, spatial arrangement and membrane subdomains as defined by confocal lectin histochemistry

Cited by 16 publications

References 20 publications

Examination of the Reticular Epithelium of the Bovine Pharyngeal Tonsil

Examination of the Reticular Epithelium of the Bovine Pharyngeal Tonsil

The role of M cells in mucosal immunity

Evidence of M cells as portals of entry for antigens in the nasopharyngeal lymphoid tissue of humans

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