Lytic bacteriophages in Veterinary Medicine: a therapeutic option against bacterial pathogens?

Borie, César; Robeson, James; Galarce, Nicolás

doi:10.4067/s0301-732x2014000200002

Cited by 19 publications

(12 citation statements)

References 50 publications

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“…Prevention of Campylobacter infections can be directly applied to humans by different ways including sewage sanitary conditions, provision of portable water, vaccine usage, public awareness concerning the significance of pasteurization of milk, proper cooking of food from animal origins and the use of therapeutics in case of infections (Hansson et al., 2018). Prevention of Campylobacter infections can also be directed on animals by phage treatment (Borie et al., 2014), probiotics, prebiotics, and by improved biosecurity such as the provision of good water quality at farm level and also by monitoring the regular use of antibiotics in animal husbandry. Another vital preventive measure that will help lower the level of these bacteria is the withholding of feed from poultry for about 12 h before slaughter (Hansson et al., 2018).…”

Section: Campylobacter Infectionsmentioning

confidence: 99%

Human campylobacteriosis: A public health concern of global importance

Igwaran

Okoh

2019

Heliyon

183

124

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Campylobacter species are among the leading cause of bacterial foodborne and waterborne infections. In addition, Campylobacter is one of the major causative agent of bacterial gastrointestinal infections and the rise in the incidence of Campylobacter infections have been reported worldwide. Also, the emergence of some Campylobacter species as one of the main causative agent of diarrhea and the propensity of these bacteria species to resist the actions of antimicrobial agents; position them as a serious threat to the public health. This paper reviews Campylobacter pathogenicity, infections, isolation and diagnosis, their reservoirs, transmission pathways, epidemiology of Campylobacter outbreaks, prevention and treatment option, antibiotics resistance and control of antibiotics use.

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Section: Campylobacter Infectionsmentioning

confidence: 99%

Human campylobacteriosis: A public health concern of global importance

Igwaran

Okoh

2019

Heliyon

183

124

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“…Phages are viruses which can target and kill bacteria, so that phages are considered as one kind of alternative for antibiotics or chemicals [ 14 ]. The first reported application of therapeutic phages dates can be traced back to the early 20th century [ 15 ] while phages were used to control a variety of diseases including diarrhea, cholera, dysentery and salmonellosis [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Characterization of Salmonella Phage LPST153 That Effectively Targets Most Prevalent Salmonella Serovars

Islam

Mizan

et al. 2020

Microorganisms

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Foodborne diseases represent a major risk to public health worldwide. In this study, LPST153, a novel Salmonella lytic phage with halo (indicative of potential depolymerase activity) was isolated by employing Salmonella enterica serovar Typhimurium ATCC 13311 as the host and had excellent lytic potential against Salmonella. LPST153 is effectively able to lyse most prevalent tested serotypes of Salmonella, including S. Typhimurium, S. Enteritidis, S. Pullorum and S. Gallinarum. Morphological analysis revealed that phage LPST153 belongs to Podoviridae family and Caudovirales order and could completely prevent host bacterial growth within 9 h at multiplicity of infection (MOI) of 0.1, 1, 10 and 100. LPST153 had a latent period of 10 min and a burst size of 113 ± 8 PFU/cell. Characterization of the phage LPST153 revealed that it would be active and stable in some harsh environments or in different conditions of food processing and storage. After genome sequencing and phylogenetic analysis, it is confirmed that LPST153 is a new member of the Teseptimavirus genus of Autographivirinae subfamily. Further application experiments showed that this phage has potential in controlling Salmonella in milk and sausage. LPST153 was also able to inhibit the formation of biofilms and it had the ability to reduce and kill bacteria from inside, including existing biofilms. Therefore, the phage LPST153 could be used as a potential antibacterial agent for Salmonella control in the food industry.

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“…It is clear that alternatives to antibiotics are urgently required to control Salmonella infection in pigs. Bacteriophages (phages) are viruses that target and kill bacteria and are one such alternative (Borie et al, 2014). Phages have a long history of being used as antimicrobials and there is an increasing interest in developing their use in animal husbandry due to their specificity, efficient bacterial lysis and their capability to self-replicate (Loc-Carrillo and Abedon, 2011; Nobrega et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Genomic Characterization of Jumbo Salmonella Phages That Effectively Target United Kingdom Pig-Associated Salmonella Serotypes

et al. 2019

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A common cause of human food poisoning is through ingestion of pork products contaminated with Salmonella spp. Worryingly multi-drug resistant (MDR) Salmonella strains have been isolated from pigs, which motivates the need for alternative antimicrobials. In this study isolation and characterization of 21 lytic Salmonella phages is described. All 21 phages, labeled as SPFM phages were shown to efficiently infect MDR Salmonella strains isolated from United Kingdom pigs and phages SPFM1, SPFM3, SPFM10, SPFM14, SPFM15, SPFM17, and SPFM19 could lyse 100% of strains tested. The phage genome sizes range from 233 to 242 Kb, which qualifies them as jumbo phages. All SPFM phage genomes are approximately 95% similar to each other by average nucleotide identity, they encode between 258–307 coding sequences and share 188 core genes. Phylogenetic analysis shows these phages are most similar to phages of the genus Seoulvirus and to further characterize phages within the genus, genes under positive selection were identified. Several of the genes under evolutionary selection pressure were predicted to encode for proteins that interact with bacteria. We describe the phenotypic and genetic characterization of this novel Salmonella phage set. As the phages efficiently kill MDR Salmonella strains, they may offer a promising alternative to antibiotics.

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Lytic bacteriophages in Veterinary Medicine: a therapeutic option against bacterial pathogens?

Cited by 19 publications

References 50 publications

Human campylobacteriosis: A public health concern of global importance

Human campylobacteriosis: A public health concern of global importance

Characterization of Salmonella Phage LPST153 That Effectively Targets Most Prevalent Salmonella Serovars

Genomic Characterization of Jumbo Salmonella Phages That Effectively Target United Kingdom Pig-Associated Salmonella Serotypes

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