Lytic bacteriophage vB_KmiS-Kmi2C disrupts biofilms formed by members of the Klebsiella oxytoca complex, and represents a novel virus family and genus

Abstract

Aims This study aimed to characterise the lytic phage vB_KmiS-Kmi2C, isolated from sewage water on a GES-positive strain of Klebsiella michiganensis. Methods and results Comparative phylogenetic and network-based analyses were used to characterise the genome of phage vB_KmiS-Kmi2C (circular genome of 42 234 bp predicted to encode 55 genes), demonstrating it shared little similarity with other known phages. The phage was lytic on clinical strains of K. oxytoca (n = 2) and K. michiganensis (n = 4), and was found to both prevent biofilm formation and disrupt established biofilms produced by these strains. Conclusions We have identified a phage capable of killing clinically relevant members of the Klebsiella oxytoca complex (KoC). The phage represents a novel virus family (proposed name Dilsviridae) and genus (proposed name Dilsvirus).

Phenotypic and genomic characterization of Pseudomonas aeruginosa isolates recovered from catheter-associated urinary tract infections in an Egyptian hospital

“…Illumina sequencing (Nextera XT Library Prep kit; HiSeq/NovaSeq; 2×250 bp paired-end reads; minimum 30× coverage, mean value after trimming and filtering of reads) was performed by microbesNG (Birmingham, UK) as described previously [ 18 ]. In brief, reads were adapter-trimmed to a minimum length of 36 nt using Trimmomatic 0.30 [ 19 ] with a sliding window quality cut-off of Q15.…”