LysR family activator-regulated major facilitator superfamily transporters are involved in Vibrio cholerae antimicrobial compound resistance and intestinal colonisation

Chen, Sushu; Wang, Hui; Katzianer, David S.; Zhong, Zengtao; Zhu, Jun

doi:10.1016/j.ijantimicag.2012.10.008

Cited by 28 publications

(21 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Another MFS transporter, called EmrD-3, was found by cloning in a drug-hypersusceptible E. coli host and produced resistance to various lipophilic agents, such as linezolid (351). Five MFS transporters (each under the control of a Lys-type MfsR regulator) whose deletion causes tetracycline and bile salt hypersusceptibility are known (352). V. parahaemolyticus was the organism in which the first MATE family transporter, NorM, was discovered by cloning in E. coli (353), and in V. cholerae, 5 members of this family were described (16), including the NorM homolog VcmA (15), which increases MICs of fluoroquinolones, ethidium bromide, acriflavine, and doxorubicin when overproduced in E. coli.…”

Section: Vibrio Sppmentioning

confidence: 99%

“…Similarly, a mutant of V. cholerae deficient in RND pumps pro-duced significantly less cholera toxin and fewer toxin-coregulated pili (341,344), and TolC mutants were deficient in intestinal colonization in mice (917) (but see the comment above on S. enterica serovar Typhimurium). Genetic deletion of one of the five MFS pump genes also produced a colonization defect of V. cholerae in mice (352). Inactivation of RND pumps in V. parahaemolyticus resulted in reduced virulence in a rabbit model (348).…”

Section: Fitness Colonization and Virulencementioning

confidence: 99%

See 1 more Smart Citation

The Challenge of Efflux-Mediated Antibiotic Resistance in Gram-Negative Bacteria

2015

View full text Add to dashboard Cite

SUMMARY The global emergence of multidrug-resistant Gram-negative bacteria is a growing threat to antibiotic therapy. The chromosomally encoded drug efflux mechanisms that are ubiquitous in these bacteria greatly contribute to antibiotic resistance and present a major challenge for antibiotic development. Multidrug pumps, particularly those represented by the clinically relevant AcrAB-TolC and Mex pumps of the resistance-nodulation-division (RND) superfamily, not only mediate intrinsic and acquired multidrug resistance (MDR) but also are involved in other functions, including the bacterial stress response and pathogenicity. Additionally, efflux pumps interact synergistically with other resistance mechanisms (e.g., with the outer membrane permeability barrier) to increase resistance levels. Since the discovery of RND pumps in the early 1990s, remarkable scientific and technological advances have allowed for an in-depth understanding of the structural and biochemical basis, substrate profiles, molecular regulation, and inhibition of MDR pumps. However, the development of clinically useful efflux pump inhibitors and/or new antibiotics that can bypass pump effects continues to be a challenge. Plasmid-borne efflux pump genes (including those for RND pumps) have increasingly been identified. This article highlights the recent progress obtained for organisms of clinical significance, together with methodological considerations for the characterization of MDR pumps.

show abstract

Section: Vibrio Sppmentioning

confidence: 99%

Section: Fitness Colonization and Virulencementioning

confidence: 99%

The Challenge of Efflux-Mediated Antibiotic Resistance in Gram-Negative Bacteria

2015

View full text Add to dashboard Cite

show abstract

“…Chen and colleagues demonstrated failure of Vibrio cholerae colonization of an infant mouse upon deletion of five MFS efflux pump genes, providing evidence for a role of these genes in pathogenesis (Chen et al, 2013). In many cases, pathogenic fungi displayed decreased pathogenicity due to loss of MFS.…”

mentioning

confidence: 99%

“…Prior studies of the MFS in plant pathogens focused on multidrug transporters. Results have shown that deletion of these proteins results in susceptibility to certain fungicides and bactericides, including exogenous toxic compounds, synthetic drugs and demethylation inhibitors (DMIs) (Chen, Wang, Katzianer, Zhong, & Zhu, 2013;Hayashi, Schoonbeek, & de Waard, 2002;Okandeji, Greenwald, Wroten, & Sello, 2011). Although little is known about sugar transporters in pathogenic fungi, members of the MFS which transport sugar have been studied in other species.…”

mentioning

confidence: 99%

See 1 more Smart Citation

A major facilitator superfamily transporter in Colletotrichum fructicola (CfMfs1) is required for sugar transport, appressorial turgor pressure, conidiation and pathogenicity

2019

View full text Add to dashboard Cite

The major facilitator superfamily (MFS) is one of the largest membrane‐protein families. To investigate the role of MFS proteins in the fungal plant anthracnose pathogen Colletotrichum fructicola, the CfMFS1 gene was deleted. This resulted in reduced mycelial growth, conidial yield and decreased virulence on tea oil camellia leaves. In addition, ∆Cfmfs1 showed increased sensitivity to osmotic stress and to a cell‐wall stressor. Further analysis revealed that CfMfs1 is required for conidial penetration and appressorial turgor pressure, both important for fungal pathogen invasion. Confocal fluorescence microscopy showed that CfMfs1 is localized to membranes of both hyphae and conidia, suggesting that it may be a membrane transporter. Our study provides evidence that CfMfs1 has a role in conidiation, sugar transport, stress response, conidial penetration, appressorial turgor pressure and virulence against tea oil camellia.

show abstract

Photocatalytic and Antibacterial Performance of α-Fe₂ O₃ Nanostructures

et al. 2017

View full text Add to dashboard Cite

Morphology engineered a-Fe 2 O 3 nanostructures with magnificent antibacterial property and excellent photocatalytic activity have been presented to address the long-standing environment issue. After tuning the morphology via solvothermal route the products were characterized by morphological, structural and elemental analysis. The visible-light photocatalytic activity of a-Fe 2 O 3 nanostructures was evaluated by using them to degrade methylene blue (MB) dye under visible light irradiation in the presence of H 2 O 2 . Amidst the series, nano bead-like structure exhibited the greatest activity toward MB degradation with 90 % degradation efficiency. Unique bead-like structure having fluctuant diameter is thought to play a key role in the enhanced separation of electron-hole pairs of a-Fe 2 O 3 and results superior photocatalytic activity. On the other hand all the shape variants of a-Fe 2 O 3 nanostructures showed brilliant antibacterial effect against Gram negative (Escherichia coli) bacterium. Based on the current findings, the presented a-Fe 2 O 3 nanostructures with their superb photocatalysis and antibacterial activity can be a promising candidate for industry to abate the ever rising environmental threat.[a] T.

show abstract

LysR family activator-regulated major facilitator superfamily transporters are involved in Vibrio cholerae antimicrobial compound resistance and intestinal colonisation

Cited by 28 publications

References 15 publications

The Challenge of Efflux-Mediated Antibiotic Resistance in Gram-Negative Bacteria

The Challenge of Efflux-Mediated Antibiotic Resistance in Gram-Negative Bacteria

A major facilitator superfamily transporter in Colletotrichum fructicola (CfMfs1) is required for sugar transport, appressorial turgor pressure, conidiation and pathogenicity

Photocatalytic and Antibacterial Performance of α-Fe₂ O₃ Nanostructures

Contact Info

Product

Resources

About

LysR family activator-regulated major facilitator superfamily transporters are involved in Vibrio cholerae antimicrobial compound resistance and intestinal colonisation

Cited by 28 publications

References 15 publications

The Challenge of Efflux-Mediated Antibiotic Resistance in Gram-Negative Bacteria

The Challenge of Efflux-Mediated Antibiotic Resistance in Gram-Negative Bacteria

A major facilitator superfamily transporter in Colletotrichum fructicola (CfMfs1) is required for sugar transport, appressorial turgor pressure, conidiation and pathogenicity

Photocatalytic and Antibacterial Performance of α-Fe2 O3 Nanostructures

Contact Info

Product

Resources

About

Photocatalytic and Antibacterial Performance of α-Fe₂ O₃ Nanostructures