Lysozyme-stabilized gold fluorescent cluster: Synthesis and application as Hg2+ sensor

Wei, Hui; Wang, Zidong; Yang, Limin; Tian, Shiliang; Hou, Changjun; Lu, Yi

doi:10.1039/c0an00046a

Cited by 410 publications

(339 citation statements)

References 37 publications

(49 reference statements)

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“…Since almost all the metal atoms in a NC are exposed, they are prone to chemical reactions, which usually lead to a change in fluorescence signal. An early work on this was reported by Ying and co-workers using BSA stabilized AuNCs [114], and then by Lu and coworkers using lysozyme stabilized AuNCs [115]. In both cases, Hg 2+ was found to strongly quench the fluorescence of AuNCs.…”

Section: Reactive Quenchersmentioning

confidence: 94%

DNA-stabilized, fluorescent, metal nanoclusters for biosensor development

Liu

2014

TrAC Trends in Analytical Chemistry

190

141

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In the past decade, fluorescent silver, gold and copper nanoclusters (Ag, Au and CuNCs) have emerged as a new class of signaling moiety for biosensor development. Compared to semiconductor quantum dots, metal NCs have less toxicity concerns and can be more easily conjugated to biopolymers. Due to their extremely small size, these NCs need a stabilizing ligand. Many polymers, proteins and nucleic acids have been reported to stabilize NCs. In particular, many DNA sequences produce highly fluorescence NCs. Coupling these DNA stabilizers with other sequences such as aptamers has generated a large number of biosensors. In this review, the synthesis of DNA and nucleotide-templated NCs is first summarized; their chemical interactions are also discussed. In the second part, the properties of NCs such as fluorescence quantum yield, emission wavelength and lifetime, structure and photostability are briefly reviewed. In the last part, various sensor design strategies using these NCs are categorized into the following four classes: 1) fluorescence de-quenching; 2) generation of templating DNA sequences to produce NCs; 3) change of nearby environment; and 4) reacting with heavy metal ions or other quenchers. Finally, the future trends in this field are discussed.

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Section: Reactive Quenchersmentioning

confidence: 94%

DNA-stabilized, fluorescent, metal nanoclusters for biosensor development

Liu

2014

TrAC Trends in Analytical Chemistry

190

141

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“…Along with monolayer protected clusters, analogous systems with macromolecular protection, especially in protein templates, have also been made. [29][30][31][32][33][34][35][36][37][38][39][40][41][42][43][44][45][46][47][48] Bovine serum albumin (BSA) [29][30][31][32][33][34][35][36] , lactoferrin (Lf) 37,38 , lysozyme (Lyz) [39][40][41][42][43][44] and some other proteins like insulin 45 are used for these kind of studies, among which BSA is most thoroughly studied.…”

Section: Introductionmentioning

confidence: 99%

Noble metal alloy clusters in the gas phase derived from protein templates: unusual recognition of palladium by gold

Baksi

Pradeep

2013

Nanoscale

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Matrix assisted laser desorption ionization of a mixture of gold and palladium adducts of the protein lysozyme (Lyz) produces naked alloy clusters of the type Au 24 Pd + in the gas phase. over all other clusters. Gas phase cluster formation occurs from protein adducts where Au is in the 1+ state while Pd is in the 2+ state. The creation of alloys in the gas phase is not affected whether a physical mixture of Au and Pd adducts or a Au and Pd co-adduct is used as the precursor. The formation of Au cores and AuPd alloy cores of the kind comparable to monolayer protected clusters imply that naked clusters themselves may be nucleated in solution.

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“…In the absence of Hg 2+ , the FAM-tagged ssDNA protected the Au NPs from salt-induced aggrega- The second frequently adopted scheme was based on the fluorescence quenching of Au NCs. Au NCs capped with lysozyme (Lys), bovine serum albumin (BSA) and dihydrolipoic acid (DHLA) have been synthesized for sensing Hg 2+ through the specific interaction between Hg 2+ (5d 10 ) and Au + (5d 10 ) on the surface of Au NCs [89][90][91][92][93]. As shown in Fig.…”

Section: Metal Nps Based Fluorescent Sensorsmentioning

confidence: 99%

Recent developments on nanomaterials-based optical sensors for Hg2+ detection

Duan

Zhan

2015

Sci. China Mater.

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Mercuric ion (Hg 2+ ), released from both natural and industrial sources, has severe adverse effects on human health and the environment even at very low concentrations. It is very important to develop a rapid and economical method for the detection of Hg 2+ with high sensitivity and selectivity. Nanomaterials with unique size and shape-dependent optical properties are attractive sensing materials. The application of nanomaterials to design optical sensors for Hg 2+ provides a powerful method for the trace detection of Hg 2+ in the environment, because these optical sensors are simple, rapidly responsive, cost-effective and highly sensitive. This review summarizes the recent advances on the development of optical assays for Hg 2+ in aqueous solution by using functionalized nanomaterials (including noble metal nanoparticles, fluorescent metal nanoclusters, semiconductor quantum dots and carbon nanodots). Detection strategies based on the Hg 2+ -induced changes in spectral absorbance, fluorescence intensity and surface-enhanced Raman scattering signals were described. And the design principles for each optical assay were presented. In addition, the future challenge and the prospect of the development of nanomaterial optical sensors for Hg 2+ detection were also discussed.

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Lysozyme-stabilized gold fluorescent cluster: Synthesis and application as Hg2+ sensor

Cited by 410 publications

References 37 publications

DNA-stabilized, fluorescent, metal nanoclusters for biosensor development

DNA-stabilized, fluorescent, metal nanoclusters for biosensor development

Noble metal alloy clusters in the gas phase derived from protein templates: unusual recognition of palladium by gold

Recent developments on nanomaterials-based optical sensors for Hg2+ detection

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