Lysozyme Content in Organs and Blood Serum in Various Species in the Volga River

Субботкина, Т. А.; Субботкин, М. Ф.

doi:10.1023/b:joey.0000015961.99374.62

Cited by 16 publications

(8 citation statements)

References 3 publications

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“…In some species, the highest lysozyme levels were recorded in kidney (Subbotkina & Subbotkin 2003) while it was not observed in the liver and spleen of cod, Gadus morhua, and haddock, Melanogrammus aeglefinus (Fange, Lundblad & Lind 1976) of course without any analysing the kidney lysozyme. In some species, the highest lysozyme levels were recorded in kidney (Subbotkina & Subbotkin 2003) while it was not observed in the liver and spleen of cod, Gadus morhua, and haddock, Melanogrammus aeglefinus (Fange, Lundblad & Lind 1976) of course without any analysing the kidney lysozyme.…”

Section: Discussionmentioning

confidence: 93%

“…The cods (Gadiformes) are characterized by very low level of the enzyme activity in some organs while the flatfish (Pleuronectiformes) showed diversity and distribution of lysozyme in the organs. The studies have established the regularity of lysozyme distribution in the sturgeon fish: kidney>spleen>liver>blood serum (Subbotkina & Subbotkin 2003). Type I was surprisingly potent, killing all the bacteria while hen egg-white lysozyme, used as a reference, killed only one bacterial species which was considered to be non-pathogenic.…”

Section: Introductionmentioning

confidence: 99%

“…Type I was surprisingly potent, killing all the bacteria while hen egg‐white lysozyme, used as a reference, killed only one bacterial species which was considered to be non‐pathogenic. The studies have established the regularity of lysozyme distribution in the sturgeon fish: kidney>spleen>liver>blood serum (Subbotkina & Subbotkin ). High activities of the lysozyme are found in lymphomyeloid tissues of elasmobranchs and also in plasma of teleosts (Saurabh & Sahoo ).…”

Section: Introductionmentioning

confidence: 99%

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Effects of temperature and gonadal growth on the lysozyme level of immune tissues in the male and female Caspian kutum (Rutilus frisii kutum )

Heidari

Farzadfar

2015

Aquac Res

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Lysozyme levels in the head kidney, spleen and liver tissues of the male and female Caspian kutum (Rutilus frisii kutum) were measured under monthly temperature variations and gonadal growth. The lysozyme content in all tissues of the male and female of the Caspian kutum showed almost a significant decreasing trend from October to March, coinciding with the lowest sea surface temperature and afterwards, it increased in April and May. In vitellogenic stage of the ovarian growth, the lysozyme level decreased in all three tissues and then increased significantly in the spawning stage. Over testicular growth, lysozyme level showed no remarkable variations from the early maturing to developed stages but increased in the spermiation stage. The regularity of lysozyme level in both sexes of the Caspian kutum was liver>spleen>head kidney. The effectiveness ratio (partial Eta 2 ) of temperature and gonadal growth in the lysozyme variations showed that temperature factor was more highlight than gonadal growth. In conclusion, the liver similar to the kidney and spleen plays an essential role against pathogen as an organ of lysozyme producing in the Caspian kutum. In addition, the female lysozyme level was higher than the male in all studied tissues.

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Section: Discussionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of temperature and gonadal growth on the lysozyme level of immune tissues in the male and female Caspian kutum (Rutilus frisii kutum )

Heidari

Farzadfar

2015

Aquac Res

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“…In our study, the order of lysozyme content in the four tissues was as follows: kidney > serum>mucus > liver. The highest content of lysozyme was possible due to many white blood cells in the fish kidney (Subbotkina & Subbotkin, 2003). Lysozyme is affected by many factors such as health status, size, sex, temperature, season and heredity of fish (Bachali et al, 2002;Palaksha, Shin, Kim, & Jung, 2008).…”

Section: Discussionmentioning

confidence: 99%

Construction expression and immunogenicity of a novel trivalent outer membrane protein (OmpU‐A‐II) from three bacterial pathogens in Japanese eels (Anguilla japonica)

Zhao

Zhai

et al. 2020

Journal of Fish Diseases

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Vibrio vulnificus, Edwardsiella anguillarum and Aeromonas hydrophila are three common bacterial pathogens in cultivated eels. To protect farming eels from infection by these pathogens, a trivalent outer membrane protein (OMP) containing partial sequences of OmpU from V. vulnificus, OmpA from E. anguillarum and OmpII from A. hydrophila was expressed and purified; then, the OMP was used as a vaccine to immunize Japanese eels (Anguilla japonica). Whole-blood cell proliferation, antibody titres and complement and lysozyme activities were detected at different days postimmunization (dpi), and the relative per cent survival (RPS) was determined after eels were infected with V. vulnificus, E. anguillarum or A. hydrophila at 28 dpi. The results showed that the OMP significantly stimulates the antibody titres. At 14 days after the challenge (i.e. at 28 dpi), the RPS of OMP against V. vulnificus, E. anguillarum and A. hydrophila was 20%, 70% and 11.1%, respectively. The construction, expression and immunogenicity of a trivalent Omp were reported for the first time, and this study will provide a valuable reference for the development of fish multiplex vaccines. K E Y W O R D SAeromonas hydrophila, Edwardsiella anguillarum, immunogenicity, trivalent Omp, Vibrio vulnificus How to cite this article: Zhao J, Wu L, Zhai S, Lin P, Guo S.Construction, expression and immunogenicity of a novel trivalent outer membrane protein (OmpU-A-II) from three bacterial pathogens in Japanese eels (Anguilla japonica). J Fish

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“…Serum lysozyme activity was measured according to the method described by Subbotkina and Subbotkin [21], and the test kit was purchased from Nanjing Jiancheng Bioengineering Institute of China. White blood cell (WBC) counts and red blood cell (RBC) counts were measured in a Mindray BC-5300 Hematology Analyzer (Mindray, China).…”

Section: Measurement Of Antioxidant Enzymes and Immune Parameters Undmentioning

confidence: 99%

Nrf2 is involved in osmoregulation, antioxidation and immunopotentiation inCoilia nasusunder salinity stress

Wang

Zhu

2019

Biotechnology & Biotechnological Equipment

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Coilia nasus is a rare anadromous fish species found in China's Yangtze River and is a precious commercial commodity. The anti-stress mechanism of C. nasus has become the focus of research due to the animal's strong stress responses. Salinity has been reported to weaken the C. nasus stress response. Nrf2 is an important anti-stress transcription factor that plays key regulatory roles in mediating salinity stress. In the present study, we cloned the full-length sequence of C. nasus Nrf2 for the first time and evaluated its differential expression in tissues. We also investigated the regulatory role of Nrf2 in osmoregulation, antioxidation and immunopotentiation in C. nasus under salinity stress. The results indicate that the C. nasus Nrf2 sequence is highly conserved. C. nasus Nrf2 is activated and plays a coordinated osmoregulatory role together with AQP1 under salinity stress and stimulates the activity of downstream antioxidant enzymes, including superoxide dismutase, glutathione peroxidase and so on. Nrf2 also triggers immunopotentiation via stimulation of lysozyme activity and an elevation of white blood cell counts. All of these C. nasus constructed adaptive Nrf2 regulatory networks act in response to salinity stress. This study lays a theoretical foundation for further research on the anti-stress mechanisms of C. nasus and will provide a reference for the further optimization of the C. nasus breeding mode.

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Lysozyme Content in Organs and Blood Serum in Various Species in the Volga River

Cited by 16 publications

References 3 publications

Effects of temperature and gonadal growth on the lysozyme level of immune tissues in the male and female Caspian kutum (Rutilus frisii kutum )

Effects of temperature and gonadal growth on the lysozyme level of immune tissues in the male and female Caspian kutum (Rutilus frisii kutum )

Construction expression and immunogenicity of a novel trivalent outer membrane protein (OmpU‐A‐II) from three bacterial pathogens in Japanese eels (Anguilla japonica)

Nrf2 is involved in osmoregulation, antioxidation and immunopotentiation inCoilia nasusunder salinity stress

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