Lysozyme coated copper nanoclusters for green fluorescence and their utility in cell imaging

Thawari, Atul Gajanan; Kumar, Piyush; Srivastava, Rohit; Rao, Chebrolu P.

doi:10.1039/d0ma00386g

Cited by 17 publications

(19 citation statements)

References 31 publications

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“…Lysozyme protected Cu nanocluster (Lys-Cu NC) was prepared in a molar ratio of 1:1 under aqueous condition following the previously reported method with some modifications. 12,53 Initially, 20 mg of lysozyme (10 mg/ml) was dissolved in 2 ml of Milli-Q water and 1 ml of CuCl2 was added from 1.…”

Section: Synthesis Of Photoluminescent Copper Nanoclustermentioning

confidence: 99%

“…Recently researchers started to overcome the challenges and synthesized stable biocompatible Cu-NCs using various functional capping agents. 8,10,12,32,33,[49][50][51][52][53][54] Compared to other proteins, Lysozyme (M.W. 14.3 kDa) is less expensive, commercially available, exhibits antibacterial property, high pH stability (isoelectric point ~11) 55,56 and strong binding affinity towards metal ions due to the presence of large number of (~56% of the total sequence) reactive side chains (-NH2, -OH, -SH, -COOH), 57 making it a suitable capping agent for the synthesis of Cu NCs, but it has not been well explored.…”

Section: Introductionmentioning

confidence: 99%

“…14.3 kDa) is less expensive, commercially available, exhibits antibacterial property, high pH stability (isoelectric point ~11) 55,56 and strong binding affinity towards metal ions due to the presence of large number of (~56% of the total sequence) reactive side chains (-NH2, -OH, -SH, -COOH), 57 making it a suitable capping agent for the synthesis of Cu NCs, but it has not been well explored. 12,52,53 Changing the protein scaffold and reaction conditions can change the properties and applications of the NCs significantly. 1,20,58 Thus there are plenty of opportunities to explore the applications of the Lys-Cu NCs.…”

Section: Introductionmentioning

confidence: 99%

“…The limited number of reports on Lys-Cu NC mostly focused on its role as an cell imaging probe, but the metal sensing ability still remains unexplored. 12,52,53 Many metal ions in our biological system have been linked to a number of diseases. [59][60][61] Thus sensitive and selective detection of biologically relevant metal ions at a very low concentration range (~nM range) has extremely important therapeutic implications.…”

Section: Introductionmentioning

confidence: 99%

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Lysozyme protected Cu Nano-Cluster: A Photo Switch for the Selective Sensing of Iron (Fe2+)

Sebastian

Aarya

Sarangi

et al. 2022

Preprint

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Protein capped metal nanoclusters gained a lot of recent attention due to their wide range of applications. Here we successfully synthesized a stable, biocompatible lysozyme protected Cu nanocluster (Lys-Cu NC) using an optimized green one-pot protocol under aqueous condition at room temperature. The nanocluster showed a strong photoluminescence intensity (λex = 365 nm, λem = 430 nm) which can be significantly and selectively quenched (off) by Fe2+ ions. Upon addition of NaOH the initial photoluminescence intensity can be recovered completely (on) thereby making the nanocluster a suitable candidate for a photo switch that can be reliably reused for the selective and sensitive detection of Fe2+ ions in the nanomolar (detection limit ~2.5 nM) concentration range. The photoluminescence intensity is also sensitive towards temperature indicating that it can be used as a temperature sensor in different biological systems. It can also be used as an excellent nanoprobe for cell imaging studies.

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Section: Synthesis Of Photoluminescent Copper Nanoclustermentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Lysozyme protected Cu Nano-Cluster: A Photo Switch for the Selective Sensing of Iron (Fe2+)

Sebastian

Aarya

Sarangi

et al. 2022

Preprint

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“…TEM can obtain subcellular imaging of heavy metal elements such platinum, but it is not sensitive enough to image iron, zinc, copper and platinum simultaneously in cells. [21][22][23] The emerging mass spectrometry imaging techniques, such as laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS), matrix-assisted laser desorption/ ionization mass spectrometry (MALDI-MS) and desorption electrospray ionization mass spectrometry (DESI-MS) are challenged to meet the requirements of single-cell measurements. In recent years, secondary ion mass spectrometry (SIMS) has attracted wide and increasing attentions especially in biology research.…”

Section: Introductionmentioning

confidence: 99%

The Disturbance of Anticancer Drug Cisplatin to Cellular Homeostasis of Trace Elements Revealed by ICP-MS and ToF-SIMS

Jia¹,

Li²,

Zhang³

et al. 2021

At.Spectrosc.

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Trace elements play important roles in many physiological processes. The disorder in the metabolism and/or homeostasis of the trace elements will cause pathogenic changes, and finally lead to the development of diseases. This present work aimed to investigate the effect of anticancer metallodrug cisplatin on the homeostasis of trace metal elements, iron (Fe), zinc (Zn) and copper (Cu), in both human HEK293 normal cells and A549 lung cancer cells by using inductively coupled plasma mass spectrometry (ICP-MS) and time-of-flight secondary ion mass spectrometry (ToF-SIMS). The levels of trace metal elements in both whole cells and nuclei subjected to cisplatin treatment were measured by ICP-MS, and results showed that accompanying with the increasing concentration of cisplatin, the content of Fe inside whole cells and nuclei of both normal and cancer cells increased, implying that cisplatin may induce ferroptosis, contributing to its anticancer activity. While the level of Cu and Zn in the whole cells increased with increase in cisplatin concentration, the level of Cu and Zn, in particular the latter, in nuclei decreased with increase of cisplatin concentration. These implicate that cisplatin may compete with Zn for binding to metalloproteins in nuclei, leading to efflux of Zn from nucleus. The normalized signal intensity of Fe, Zn, and Cu in single cells detected by ToF-SIMS increased accompanying with increased cisplatin concentration, being in line with that obtained by quantitative ICP-MS on large quantity of cells. These findings provide novel insights into better understanding in the mechanism of action of cisplatin.Trace metal elements, such as iron, zinc, copper, are essential in the human body as they consist of many metalloproteins or enzymes which are involved in vital physiological regulation processes and play crucial roles in maintaining the normal function

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Design of Lysozyme‐Templated Biocompatible Fluorescent Nanocomposites and Their Potential Applications for Cell Imaging, Hydrogen Peroxide Sensing, and Cancer Therapy

Das

Tripathy

Sreedhar

et al. 2023

Advanced Therapeutics

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Recently, multifunctional metal‐based nanocomposites gained immense importance in various biomedical applications. Here, a simple approach is reported to synthesize fluorescent bimetallic nanocomposites (NCs) of silver‐gold (Ag@Au NC) and silver‐platinum (Ag@Pt NC) by reducing silver nitrate (AgNO3) on gold nanoparticles and platinum nanoparticles (AuNPs, PtNPs), respectively, in the presence of lysozyme that acts as capping agent. Various in vitro (in normal cells including Chinese hamster ovarian and human umbilical vein endothelial cells) and in vivo (in mouse and chicken egg chorioallantoic membrane model) studies reveal the biocompatibility of these nanocomposites. However, these NCs show inhibition of cancer cell proliferation when treated to various cancer cells (MCF‐7, MDA‐MB‐231, and B16F10), indicating their anticancer property. Intraperitoneal administration of these NCs resulted in the regression of melanoma tumor in C57BL/6J mouse model. Further, these NCs displayed green fluorescence inside MCF‐7 cells facilitating in vitro live‐cell imaging and sense hydrogen peroxide (H2O2) in cellular level (B16F10 and MCF‐7) using their fluorescence enhancement property in presence of H2O2.The results (in vitro and in vivo) altogether demonstrate the promising multifunctional applications of bimetallic nanocomposites (NCs) that can be utilized as promising transformative drugs in cancer theranostics applications.

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Lysozyme coated copper nanoclusters for green fluorescence and their utility in cell imaging

Abstract: Fluorescent, pH dependent and water soluble copper nanoclusters (CuNCs) were synthesized using lysozyme (lyz) as the stabilizing agent to give lysozyme coated copper nanoclusters, viz., lyz–CuNCs. The lyz–CuNCs were 3-5...

Cited by 17 publications

References 31 publications

Lysozyme protected Cu Nano-Cluster: A Photo Switch for the Selective Sensing of Iron (Fe2+)

Lysozyme protected Cu Nano-Cluster: A Photo Switch for the Selective Sensing of Iron (Fe2+)

The Disturbance of Anticancer Drug Cisplatin to Cellular Homeostasis of Trace Elements Revealed by ICP-MS and ToF-SIMS

Design of Lysozyme‐Templated Biocompatible Fluorescent Nanocomposites and Their Potential Applications for Cell Imaging, Hydrogen Peroxide Sensing, and Cancer Therapy

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