Lysozyme binding to poly(4-vinyl-N-alkylpyridinium bromide)

Silva, Rubens A.; Urzúa, Marcela; Petri, Denise Freitas Siqueira

doi:10.1016/j.jcis.2008.10.087

Cited by 21 publications

(31 citation statements)

References 37 publications

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“…Regarding the results found for LbL onto Si/SiO 2 wafers, some important information can be extracted from Table 1. After 60 min, systems composed of QPVP-C2 chains behaved similarly to those containing QPVP-C5 chains, with maximal Ds values of $95%, which is in agreement with previous results, 24 and indicate a similar amount of pyridinium groups exposed to the aqueous dispersion. The biocidal activities of the 1st, 3rd and 5th layers of QPVP-C2 or QPVP-C5 could be detected after 15 min.…”

Section: 33-36supporting

confidence: 92%

“…If a small area is used, the method becomes less sensitive. 24 After each interval of time the turbidity of M. luteus dispersions was measured (s t ). Bacteria disruption indicated antimicrobial activity 28 and was correlated with the relative decrease of turbidity (Ds); the largest is Ds, the more efficient is the antimicrobial agent:…”

Section: Evaluation Of Antimicrobial Effectmentioning

confidence: 99%

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Structural aspects of polyanion and hydrophobically modified polycation multilayers on hydrophilic or hydrophobic surfaces

et al. 2012

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Multilayer films of carboxymethylcellulose (CMC), a polyanion, and bromide salts of poly(4-vinylpyridine) quaternized with linear aliphatic chains of 2 (ethyl) and 5 (pentyl) carbon atoms, coded as QPVP-C2 and QPVP-C5, respectively, were fabricated by layer-by-layer (LbL) self-assembly onto Si/SiO 2 wafers (hydrophilic substrate) or polystyrene, PS, films (hydrophobic substrate). The films were characterized by means of ex situ and in situ ellipsometry, atomic force microscopy (AFM), contact angle measurements and sum frequency generation vibrational spectroscopy (SFG). Antimicrobial tests were used to assess the exposure of pyridinium moieties to the aqueous medium. In situ ellipsometry indicated that for Si/SiO 2 the chains were more expanded than the PS films and both substrates systems composed of QPVP-C5 were thicker than those with QPVP-C2. For dried layers, the alkyl side group size had a small effect on the thickness evolution, regardless of the substrate. At pH 2 the multilayers showed high resistance, evidencing that the build-up is driven not only by cooperative polymer-polymer ion pairing, but also by hydrophobic interactions between the alkyl side chains. The LbL films became irregular as the number of depositions increased. After the last deposition, the wettability of QPVP-C2 or QPVP-C5 terminated systems on the Si/SiO 2 wafers and PS films were similar, except for QPVP-C2 on Si/SiO 2 wafers. Unlike the morphology observed for LbL films on Si/ SiO 2 wafers, PS induced the formation of porous structures. SFG showed that in air the molecular orientation of pyridinium groups in multilayers with QPVP-C5 was stronger than in those containing QPVP-C2. The exposure of pyridinium moieties to the aqueous medium was more pronounced when the LbL were assembled on Si/SiO 2 wafers.

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Section: 33-36supporting

confidence: 92%

Section: Evaluation Of Antimicrobial Effectmentioning

confidence: 99%

Structural aspects of polyanion and hydrophobically modified polycation multilayers on hydrophilic or hydrophobic surfaces

et al. 2012

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“…In the case of pure QPVP-C5, chains dissolved in aqueous dispersion, presenting maximal "! values (96±2%), which is in agreement with studies previously reported [16]. The "!…”

Section: Antimicrobial Activity Of Cmcab/qpvp-c5 Blendssupporting

confidence: 93%

Carboxymethylcellulose acetate butyrate/poly(4-vinyl-N-pentyl pyridinium bromide) blends as antimicrobial coatings

Blachechen¹,

Amim²,

Lincopán³

et al. 2015

Express Polym. Lett.

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“…The enzymatic activity of immobilized enzymes increased with the increase of enzyme concentration in the xanthan solution and the immobilized enzymes presented higher thermal stability . Xanthan hydrogels proved to be efficient carriers for lysozyme (LYZ), an enzyme with isoelectric point (pI) at 10.7 and biocidal properties . At pH 7 the release of LYZ tends to be very low due to attraction between positively charged residues of LYZ and negatively charged xanthan .…”

Section: Xanthan Gum Networkmentioning

confidence: 99%

Xanthan gum: A versatile biopolymer for biomedical and technological applications

Petri

2015

J of Applied Polymer Sci

311

166

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Xanthan gum is an extracellular polymer produced mainly by the bacterium Xanthomonas campestris. Traditionally it plays an important role in industrial applications as thickener, emulsion stabilizer and it has been added to water-based drilling fluids due to its pseudoplastic behavior and thermal stability. The structural properties of xanthan in solution can be tuned by the temperature and ionic strength; under high ionic strength or low temperature, xanthan chains are arranged in helical conformation, whereas under low ionic strength or high temperature, xanthan chains are coiled. Xanthan high molecular weight favors the building up of physical and chemical networks, which have been used as carriers for drugs and proteins and as scaffolds for cells. In combination with other polymers xanthan has been applied as excipient in tablets or as supporting hydrogels for drug release applications, particularly due to its acid resistance. The large versatility of xanthan gum opens the possibility for the creation of new architectures and additional applications involving this fascinating polymer. AIMSThe purpose of this review is to report on the applications of xanthan gum in biomedical and technological products over the last ten years, but revisiting the background and the highlights of traditional applications as thickener. The in vivo biodegradability and biocompatibility of xanthan gum are fundamental characteristics that allow its use in biomedical applications. The ability to form networks and acid resistance make xanthan gum, as pure excipient or in combination with other polymers, very attractive as drug carriers. The recent combinations with inorganic particles open a range of new applications.

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Lysozyme binding to poly(4-vinyl-N-alkylpyridinium bromide)

Cited by 21 publications

References 37 publications

Structural aspects of polyanion and hydrophobically modified polycation multilayers on hydrophilic or hydrophobic surfaces

Structural aspects of polyanion and hydrophobically modified polycation multilayers on hydrophilic or hydrophobic surfaces

Carboxymethylcellulose acetate butyrate/poly(4-vinyl-N-pentyl pyridinium bromide) blends as antimicrobial coatings

Xanthan gum: A versatile biopolymer for biomedical and technological applications

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