Lysozyme Associated Liposomal Gentamicin Inhibits Bacterial Biofilm

Abstract

Bacteria on living or inert surfaces usually form biofilms which make them highly resistant to antibiotics and immune clearance. Herein, we develop a simple approach to overcome the above conundrum through lysozyme-associated liposomal gentamicin (LLG). The association of lysozyme to the surface of liposomes can effectively reduce the fusion of liposomes and undesirable payload release in regular storage or physiological environments. The LLG was more effective at damaging established biofilms and inhibiting biofilm formation of pathogens including Gram-positive and Gram-negative bacteria than gentamicin alone. This strategy may provide a novel approach to treat infections due to bacterial biofilm.

Silica–gentamicin nanohybrids: combating antibiotic resistance, bacterial biofilms, and in vivo toxicity

“…8 The SiO 2 -G nanohybrids thus possess superior antibiofilm effects compared with pristine gentamicin. A recent study 67 has reported a change in the surface charge of liposomal gentamicin from −54.5 to 17.5 mV after association with lysozyme and speculated the role of this surface charge in the antibiofilm effects of the lysozyme-associated liposomal gentamicin. The change in surface charge might facilitate an electrostatic attraction of the positively charged lysozyme-associated liposomal gentamicin to the negative charge of alginate in the biofilm matrix.…”

Impact of nanosystems in Staphylococcus aureus biofilms treatment

“…2014 ) and fluorescence microscopy (Hou et al . 2017 ). The biofilm biomass can also be determined by in vitro assays, such as the crystal violet staining method (Boda et al .…”

“…More recently, Hou et al . ( 2017 ) designed a gentamicin-loaded liposomal formulation with lysozyme surface functionalization. The cationic lysozyme was associated to the negatively charged liposome through electrostatic attraction.…”

Next-Science: A Novel Antimicrobial Agent that Inhibits Biofilm Development by Escherichia coli Clinical Isolates on Urinary Tract Catheters

“…An ideal approach in this regard is to utilize two agents; one that penetrates/destroys the EPS matrix (such as lysozyme) and another to kill the individual bacteria within the biofilm (such as antibiotics) [17,18]. However, as we previously demonstrated through in vitro and in vivo biofilm analyses NS is unique in that it contains both activities [11].…”