Lysozyme as a recognition element for monitoring of bacterial population

Preparation of a balsa-lysozyme eco-friendly dressing and its effect on wound healing

“…Although E group was 19.46 AE 4.21 , it still has strong hydrophilicity, which can target the internal hydrophobicity of bacteria to allow lysozyme's antibacterial function. 7,8 As shown in Fig. 5, with increased lysozyme concentration, the drug loading rate of wood-lysozyme increased gradually and the encapsulation efficiency decreased gradually (P < 0.05).…”

“…Because lysozyme is embedded in balsa structure by glue method. 8,11 The encapsulation structure is poor in density, and lysozyme has good water solubility. The lysozyme-balsa was cultured in PBS buffer solution and the lysozyme was easily released to PBS through the balsa surface structure, resulting in an increase in release.…”

“…6,7 It has been widely used as an antibacterial agent. 8 Lysozyme degrades peptidoglycan in the bacterial cell wall, leading to rapid killing of Gram-positive organisms. However, lysozyme is inactive against most Gram-negative bacteria because it cannot penetrate the outer membrane to reach its target, the peptidoglycan layer.…”

LYG1 exerts antitumor function through promoting the activation, proliferation, and function of CD4+ T cells

“…To evaluate the bacteriolytic ability of LYG1, an enzymatic assay that lysed bacteria was performed using M. lysodeikticus, which is a typical enzymatic substrate for lysozyme and is generally used to detect bactericidal activity. [24][25][26][27] As shown in Fig. S2, rhLYG1 could not lyse M. lysodeikticus, while human lysozyme (positive control) exhibited high bacteriolytic activities.…”

“…0.015% lyophilized M. lysodeikticus suspension in 66 mM potassium phosphate buffer, pH 6.24 (buffer A) was prepared at 25 C to obtain a constant absorbance between 0.7 and 0.8 at 450 nm. Different final concentrations of LYG1 or human lysozyme (Sigma, L6876) were prepared in cold buffer A and were added into the cuvettes containing the bacterial suspension.…”