Lysozyme activity in the presence of nonionic detergent micelles

Abstract

SummaryThe effect of a nonionic surfactant, polyoxyethylenesorbitan monolaurate (Tween 20), on the hen egg-white lysozyme catalyzed lysis of a dried cell suspension of Micrococcus lysodeikticus is analyzed. A rate enhancement of up to 70% is observed in the presence of surfactant a t concentrations above the critical micelle concentration. This activity increase may be explained by postulating the existence of a micelle-enzyme complex in which enzyme molecules are bound to micelles with preferential orientation of their active sites.A mechanism is postulated in which a substrate particle is assumed to be an energy-furnishing collision partner to the enzyme-substrate complex. This mechanism correlates data over a wide range of enzyme and substrate concentrations.Data from kinetic, ultrafiltration, ultraviolet, and fluorescence studies provide convincing evidence for the existence of a micelle-lysozyrne complex. The results suggest that it is possible that immobilized enzymes may in general be more reactive than corresponding free enzymes.The reaction is found to be second order with respect to substrate.

The characterization and interconversion of three forms of cholesterol oxidase extracted from Nocardia rhodochrous

“…Similar surface-dilution kinetics have been described for phospholipase A2 and phosphatidylserine carboxylase (Dennis, 1973;Warner & Dennis, 1975). Bernath & Vieth (1972) have described a similar enhancement in lysozyme activity in the presence of surfactant concentrations in excess of the critical micelle concentration. They believed that activation of the enzyme was due to an orientation effect inside the mixed micelle.…”

Effects of ink characteristics and piezo-electric inkjetting parameters on lysozyme activity

“…To maintain proper surface tension, non-ionic surfactants are preferred for enzyme-based inks as they would cause less unwanted interaction with the protein conformation 14 . The concentration of surfactant used in the ink solution could also be a critical factor to influence enzymatic activity 26 . However, to our knowledge, there are no studies concerning the factor of surface tension on the activity of inkjetted enzyme.…”

Effects of Fungal Lytic Enzymes and Non-ionic Detergents on the Actions of Some Fungicides againstPyricularia oryzae

“…Pyricularia oryzae P 21) and Glomerella cingulata G 4-1-4 3 ) were used. Botrytis cinerea, Colletotrichum lagenalium and Alternaria kikuchiana Y -33 (Polyoxins tolerant strain) were supplied by Kumiai Chemical Industry Co., Ltd. Tokyo.…”