Lysosomes in L cells infected with Chlamydia psittaci 6BC strain

Abstract

KORDOVA, N., J. C. WILT, and M. SADIQ. 1971. Lysosomes in Lcells infected with Chlamydiapsittaci 6BC strain. Can. J. Microbiol. 17: 955-959. Studies on lysosomal activation of Chlamydia psittaci infected L cells have been performed during the growth cycle of the agent and extended for 4 days after the initiation of infection. The present results Indicate that lysosomes of infected L cells were not injured during phagocytosis, but that lysosomal enzyme release f i s t occurred about 24 h after infection and apparently preceded the death and lysis of host cells. These observations attach a new significance to the study of injury and death of chlamydial infected cultured cells as well as to studies on the pathogenesis of psittacosis at the whole host level.

Lysosomes and the "toxicity" of Rickettsials. II. Non-cytocidal interactions of egg-grown C. psittaci 6BC and in vitro macrophages

“…activation of lysosomes and the genesis of the CPE in L cells, and of a cytotoxic effect in mouse phagocytes in vitro, using a cytopathic L-cell attenuated strain of C. psittaci 6BC (14,15). The present report represents an extension of our initial studies to assess the pathogenicity or toxicity of Chlamydiae for macrophages in vitro, and describes the lysosomal response of macrophages after infection with a non-cytopathic egg-attenuated 6BC strain.…”

Preliminary characterization of a chlamydial agent isolated from embryonated snow goose eggs in northern Canada

“…4). Hence, the behavior of the isolates in cultured mammalian cells appeared to be similar to the effect of the eggattenuated, noncytopathic avian C. ysittaci ~B C strain assayed in the same host systems (19,21).…”

“…It seemed of interest to examine the behavior of the goose isolates at the cellular level, in indicator host systems which we have used to differentiate cytopathic from non-cytopathic variant strains of C. ysittaci 6BC (18)(19)(20)(21). For this purpose, the partially purified inoculum obtained after three subpassages of the goose egg isolates (from dead goose embryos) in the yolk sac of chicken embryos was used, and was assayed in cultured peritoneal mouse macrophages as well as in L cells.…”

“…Eighteen of 24 mouse cell) the viability, cytomorphology, and lysosomal inoculated chicken yolk sacs contained conactivation of macrophages were determined. siderable numbers of Giemsa-or Gimtnez-L Cells stained intracellularly located pleomorphic Chla-L cells (clone 929) were purchased from the American mydiae-like bodies after the first subpassage Type Culture Collection and cultivated as previously described (19). of the isolates.…”

“…Infectivity of the goose isolates for L cells was deter-10g/ml), only 4 of 24 embryos died after the mined using the partially purified agent and previously third Passage, as estimated by the single dilution described methods of inoculation (19,21). technique (10-2/0.25 ml per egg), although…”