Lysosomal Membrane Stability and Metallothioneins in Digestive Gland of Mussels (Mytilus galloprovincialis Lam.) as Biomarkers in a Field Study

Petrović, Siniša; Ozretić, Bartolo; Krajnović-Ozretić, Mirjana; Bobinac, Dragica

doi:10.1016/s0025-326x(01)00167-9

Cited by 113 publications

(46 citation statements)

References 22 publications

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“…해산 이매패류는 어류나 다른 무척추동물에 비해 활동 범위가 제한적인 고착성의 여과 섭식자로 연안 환경 오염 에 대한 지표종(sentinel species)으로 널리 사용되고 있다 (Krishnakumar et al 1994;Petrovi et al 2001;Kagley et al 2003;Moore et al 2004;Viarengo et al 2007). 해산 이매패류는 수중으로부터 다양한 생체이물질(xenobiotics) 과 독성 화합물을 여과 섭식하여 소화맹낭 혹은 그 주변 조직 세포에 축적하며, 축적된 물질은 패류의 생리학적 기 능에 영향을 미친다 (O'Connor and Lauenstein 1989;Livingstone 1991;Petrovi et al 2001;Kagley et al 2003; Moore et al 2004).…”

Section: 서 론unclassified

“…해산 이매패류는 수중으로부터 다양한 생체이물질(xenobiotics) 과 독성 화합물을 여과 섭식하여 소화맹낭 혹은 그 주변 조직 세포에 축적하며, 축적된 물질은 패류의 생리학적 기 능에 영향을 미친다 (O'Connor and Lauenstein 1989;Livingstone 1991;Petrovi et al 2001;Kagley et al 2003; Moore et al 2004). 참굴 소화맹낭 상피 세포는 소 화 세포(digestive cell or secretory-absorbtive cell)와 호염 성 세포(basophil cell)로 구성되며, 그 모양은 길고 원통형 으로 섬모가 없다 (Winstead 1995;Eble and Scro 1996).…”

Section: 서 론unclassified

“…또한 해산 이매 패류의 소화맹낭은 먹이의 소화, 흡수뿐만 아니라 오염물 질의 해독 및 배출 작용에도 관여한다 (Livingstone 1991;Krishnakumar et al 1994). 이 소화맹낭 기능의 주된 역할 은 소화맹낭 상피세포에 존재하는 리소솜(lysosomes)이 수행한다 (Moore 1985;Viarengo et al 1987;Petrovi et al 2001).…”

Section: 서 론unclassified

“…생체이물질 혹은 환경오염 등의 스트레스를 받은 생물체의 리소솜은 리소솜 막 안정성이 저하되면서 리소솜 내 가수분해효소 가 세포질로 확산되거나 Neutral Lipids(NL) 혹은 Lipofuscin(LF)과 같은 물질이 비정상적으로 리소솜 내에 축적된다. 그 결과 병리학적인 자가탐식(autophagy) 혹은 세포 사멸(cell death), 리소솜 저장병(lysosomal storage disease) 등 세포에 해로운 영향을 미친다 (Petrovi et al 2001;Chung 2003;Cuervo 2004;Moore et al 2007).…”

Section: 서 론unclassified

“…혈 구)를 이용하여 Neutral Red(NR) 염료가 세포에서 빠져 나오는 시간(Neutral Red Retention time, NRR time)을 측정하는 방법으로, 스트레스를 받을수록 NRR time이 짧 아지게 된다. 이 측정 방법은 해산 이매패류(Ostrea edulis, Crassostrea gigas, Pecten maximus)에 활용된 바 있다 (Hauton et al 2001;Jo et al 2002;Moore et al 2004;Cho and Jeong 2005 (Moore 1988;Krishnakumar et al 1994;Petrovi et al 2001;Kagley et al 2003;Moore et al 2004). 담치(Mytilus edulis)의 경우, 소화맹낭 세포의 LMS는 환경오염물질 노출지역에서 대 조지역에 비해 리소솜 막 불안정화 시간(labilization time) 이 짧아지며, 인위적으로 오염물질의 농도 구배를 준 경우 에도 오염물질의 농도가 높을수록 리소솜 막 불안정화 시 간은 짧아진다는 것이 보고된 바 있다 (Moore 1988;Krishnakumar et al 1994;Petrovi et al 2001;Kagley et al 2003).…”

Section: 서 론unclassified

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Cytochemical Measurement of Lysosomal Responses in the Digestive Cells of Wild Pacific Oyster, Crassostrea gigas on the South Coast of Korea

Jeung¹,

Lee²,

Hong³

et al. 2012

Ocean and Polar Research

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: Digestive cells of the bivalves have a highly developed lysosomal system and the system is known to be sensitive to changes in environmental qualities. In this study, we measured lysosomal responses of the digestive cells in wild oyster, Crassostrea gigas using frozen section. Oysters were collected in June 2010 from intertidal areas in the inner and outer bay of Gwangyang off the south coast of Korea. From the tissue sections, we measured the digestive cell lysosomal membrane stability (LMS), level of neutral lipids (NL), lipofuscin (LF) and the digestive gland atrophy (DGA). The DGA and condition index of oysters from the inner bay were significantly lower (P<0.05). The statistical test indicated that LMS levels of oysters in the inner bay and the outer bay were not significantly different since a shorter activity was displayed by oysters from the inner bay than that of oysters in outer bay. The LF deposition level of the oysters in the inner bay displayed significantly higher levels than the outer bay (P<0.05). In contrast, the NL accumulation measured from oysters in outer bay was significantly higher than the level observed in the inner bay (P<0.05). Different levels of DGA and LF that were observed in the inner and outer bays were thought to be associated to different level of environmental contamination and these two assays are considered to be good biomarkers.

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Section: 서 론unclassified

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Cytochemical Measurement of Lysosomal Responses in the Digestive Cells of Wild Pacific Oyster, Crassostrea gigas on the South Coast of Korea

Jeung¹,

Lee²,

Hong³

et al. 2012

Ocean and Polar Research

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Multibiomarker response shows how native and non‐native freshwater bivalves differentially cope with heat‐wave events

Ferreira‐Rodríguez

Fernández

Cancela

et al. 2018

Aquatic Conservation

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Temperature has a key impact on the physiology, food acquisition, metabolic rate, growth, and reproduction of organisms. Thus, exploring the effects of climate change on species physiology is important for the conservation and management of native and non‐native bivalve species. In this work, an integrative laboratory approach was used to compare the physiological, cellular, and molecular responses of the native Unio delphinus and the invasive non‐native Corbicula fluminea under a natural heat‐wave event. Whereas the filtration rate in C. fluminea was clearly affected throughout the whole heat‐wave event (from the heat peak onwards), it was only significantly affected during the heat‐wave climax in U. delphinus, and recovered afterwards. At the cellular level, lysosomal membrane stability was affected during the heat‐wave climax, but recovered afterwards, in both native and non‐native bivalve species. At the molecular level, hsp70 and hsp90 gene expression were increased and decreased (respectively) in C. fluminea after the heat wave. In contrast, only hsp90 gene expression decreased in U. delphinus during the climax and after the heat wave. The present work demonstrates that accurate monitoring of native and non‐native species using an integrated biomarker approach provides new insights into how both species might cope with the expected increase in frequency and intensity of heat events in a global warming context. Direct and indirect impacts of heat waves have been associated with massive mortalities of both native and non‐native species. Despite present results showing that C. fluminea are more sensitive to heat waves than U. delphinus, massive mortality events might act in favour of C. fluminea as it has a higher reproductive capacity. Although native freshwater mussel species conservation is already threatened by overexploitation, flow regulation and diversions, habitat destruction, and pollution, it is probably also adversely affected by global warming and the presence of invasive non‐native species, such as C. fluminea.

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Physiological and cellular responses of oysters (Crassostrea hongkongensis) in a multimetal‐contaminated estuary

Wang

2016

Enviro Toxic and Chemistry

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The Pearl River estuary, southern China, suffers from multiple sources of metal contamination as a result of the rapid industrial development in the region; but the biological impacts of contamination remain unknown. In the present study, a euryhaline oyster, Crassostrea hongkongensis, was collected from different sites of the Pearl River estuary; and various physiological (heart rate, alkaline phosphatase as homeostatic regulation, and glycogen as energy reserve) and cytological (lysosomal membrane stability) biomarkers were quantified to assess this species as a potential bioindicator of metal pollution in contaminated areas. Large variations of metal accumulation levels in the oysters were documented, especially for copper (Cu), zinc (Zn), cadmium (Cd), chromium, and nickel (Ni). Among these metals, the authors demonstrated significant correlations between the digestive gland metal accumulation of Cu, Zn, and Ni and the cellular homeostasis (alkaline phosphatase) and glycogen reserves. Heart rate was positively correlated with Cd but negatively correlated with Cu and Zn concentrations in the gills. Lysosomal membrane stability was significantly inhibited at the most contaminated sites but had no relationship with the accumulated metal concentrations. These measurements indicate that multimetal contamination in the Pearl River estuary impacts the physiological and cytological performance of oysters. Environ Toxicol Chem 2016;35:2577-2586. © 2016 SETAC.

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Lysosomal Membrane Stability and Metallothioneins in Digestive Gland of Mussels (Mytilus galloprovincialis Lam.) as Biomarkers in a Field Study

Cited by 113 publications

References 22 publications

Cytochemical Measurement of Lysosomal Responses in the Digestive Cells of Wild Pacific Oyster, Crassostrea gigas on the South Coast of Korea

Cytochemical Measurement of Lysosomal Responses in the Digestive Cells of Wild Pacific Oyster, Crassostrea gigas on the South Coast of Korea

Multibiomarker response shows how native and non‐native freshwater bivalves differentially cope with heat‐wave events

Physiological and cellular responses of oysters (Crassostrea hongkongensis) in a multimetal‐contaminated estuary

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